247 research outputs found
Electron paramagnetic resonance studies of single crystals of Mn -doped (NH ) SO , Gd -doped NH Pr(SO ) .4H O,VO -doped M(NH ) (SO ) .6H O (M = Cd, Co, Fe, Mg, Zn) and Mg(ND ) (SO ) .6D O, and Cu -doped MNa (SO ) .4H O (M = Co, Mg)
Biochemical Issues in Estimation of Cytosolic Free NAD/NADH Ratio
Cytosolic free NAD/NADH ratio is fundamentally important in maintaining cellular redox homeostasis but current techniques cannot distinguish between protein-bound and free NAD/NADH. Williamson et al reported a method to estimate this ratio by cytosolic lactate/pyruvate (L/P) based on the principle of chemical equilibrium. Numerous studies used L/P ratio to estimate the cytosolic free NAD/NADH ratio by assuming that the conversion in cells was at near-equilibrium but not verifying how near it was. In addition, it seems accepted that cytosolic free NAD/NADH ratio was a dependent variable responding to the change of L/P ratio. In this study, we show (1) that the change of lactate/glucose (percentage of glucose that converts to lactate by cells) and L/P ratio could measure the status of conversion between pyruvate + NADH and lactate + NAD that tends to or gets away from equilibrium; (2) that cytosolic free NAD/NADH could be accurately estimated by L/P only when the conversion is at or very close to equilibrium otherwise a calculation error by one order of magnitude could be introduced; (3) that cytosolic free NAD/NADH is stable and L/P is highly labile, that the highly labile L/P is crucial to maintain the homeostasis of NAD/NADH; (4) that cytosolic free NAD/NADH is dependent on oxygen levels. Our study resolved the key issues regarding accurate estimation of cytosolic free NAD/NADH ratio and the relationship between NAD/NADH and L/P
On the relation between the mass of Compact Massive Objects and their host galaxies
Supermassive black holes and/or very dense stellar clusters are found in the
central regions of galaxies. Nuclear star clusters are present mainly in faint
galaxies while upermassive black holes are common in galaxies with masses M. In the intermediate galactic mass range both types of
central massive objects (CMOs) are found. Here we present our collection of a
huge set of nuclear star cluster and massive black hole data that enlarges
significantly already existing data bases useful to investigate for
correlations of their absolute magnitudes, velocity dispersions and masses with
structural parameters of their host galaxies. In particular, we directed our
attention to some differences between the correlations of nuclear star clusters
and massive black holes as subsets of CMOs with hosting galaxies. In this
context, the mass-velocity dispersion relation plays a relevant role because it
seems the one that shows a clearer difference between the supermassive black
holes and nuclear star clusters. The has a slope of while has the much smaller slope of .
The slopes of the CMO mass- host galaxy B magnitude of the two types of CMOs
are indistinguishable within the errors while that of the NSC mass-host galaxy
mass relation is significantly smaller than for supermassive black holes.
Another important result is the clear depauperation of the NSC population in
bright galaxy hosts, which reflects also in a clear flattening of the NSC mass
vs host galaxy mass at high host masses.Comment: 12 pages, 22 figures, 2 tables, accepted for publication in MNRA
Expression of natriuretic peptide receptor-A in esophageal squamous cell carcinomas and the relationship with tumor invasion and migration
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