MicroRNAs Up-Regulated by CagA of Helicobacter pylori Induce Intestinal Metaplasia of Gastric Epithelial Cells

CagA of Helicobacter pylori is a bacterium-derived oncogenic protein closely associated with the development of gastric cancers. MicroRNAs (miRNAs) are a class of widespread non-coding RNAs, many of which are involved in cell growth, cell differentiation and tumorigenesis. The relationship between CagA protein and miRNAs is unclear. Using mammalian miRNA profile microarrays, we found that miRNA-584 and miRNA-1290 expression was up-regulated in CagA-transformed cells, miRNA-1290 was up-regulated in an Erk1/2-dependent manner, and miRNA-584 was activated by NF-κB. miRNA-584 sustained Erk1/2 activities through inhibition of PPP2a activities, and miRNA-1290 activated NF-κB by knockdown of NKRF. Foxa1 was revealed to be an important target of miRNA-584 and miRNA-1290. Knockdown of Foxa1 promoted the epithelial-mesenchymal transition significantly. Overexpression of miRNA-584 and miRNA-1290 induced intestinal metaplasia of gastric epithelial cells in knock-in mice. These results indicate that miRNA-584 and miRNA-1290 interfere with cell differentiation and remodel the tissues. Thus, the miRNA pathway is a new pathogenic mechanism of CagA

Controllable adsorption of reduced graphene oxide onto self-assembled alkanethiol monolayers on gold electrodes: Tunable electrode dimension and potential electrochemical applications

This paper describes a facile and effective method to construct graphene nanosheet film (GNF) by controllable adsorption of reduced graphene oxide (rGO) onto the self-assembled monolayer (SAM) of n-octadecyl mercaptan (C(18)H(37)SH) at An electrodes. Nyquist plots show a gradual decrease of the charge transfer resistance (R(ct)) of the [Fe(CN)(6)](3-/4-) redox couple at the GNF/SAM electrode with prolonging the self-assembly time, Suggesting the controllable adsorption of rGO onto the SAM. Cyclic voltammetry (CV) studies reveal that the GNF/SAM electrodes have tunable dimensions ranging from a nanoelectrode ensemble to a conventional electrode, depending oil the self-assembly time of rGO. The excellent electrocatalytic activity of the GNF/SAM electrode toward ascorbic acid, dopamine, and uric acid further indicates that our approach is successful for the fabrication of stable GNF with excellent electrochemical properties, which is very attractive for electrochemical Studies and electroanalytical applications. At the same time, as a new kind of nanosheet film electrode, the GNF electrode Could be exploited in a new field for micro- and nanoelectrodes in electrochemical investigations and practical applications, e.g., electroanalysis in vivo and in vitro

A GM-Based Energy Management Strategy of Hybrid Power System for Hydrogen Fuel Cell Buses

Hydrogen energy is a clean, carbon-free, flexible, efficient, and widely used secondary energy source, which is an ideal alternative to promote the clean and efficient use of traditional fossil fuels. Hydrogen fuel cell bus has the advantages of a high-energy conversion rate, absolute pollution-free, sufficient raw materials, and convenient filling. The hybrid power system, composed of fuel cell and auxiliary energy source, is one of the key technologies to promote the development of hydrogen fuel cell vehicle. This study aims to propose an energy management strategy by analyzing the output characteristics and power allocation of fuel cell and power battery in the hybrid power mode with fuel cell as the main and battery as the auxiliary. A GM (1, N) power prediction strategy was proposed and compared with other strategies as an on-off control strategy and logical threshold value strategy in this study. The variation curves of the battery SOC and fuel cell output power under two working conditions of CCBC and real vehicle conditions were analyzed by using these three strategies, when the initial SOC of power battery is 30%, 70%, and 90%, respectively. Results showed that the power prediction strategy based on GM (1, N) has a better performance in output efficiency and fuel economy when compared to the other two strategies by analyzing the aspects of the battery in the SOC variation and equivalent hydrogen consumption and the fuel cell in the output power variation and hydrogen consumption. This research can be helpful to provide the suggested solution for energy management of the hybrid power system for hydrogen fuel cell buses

A graphene modified anode to improve the performance of microbial fuel cells

Graphene with a Brunauer-Emmett-Teller (BET) specific surface area of 264 m g has been used as anodic catalyst of microbial fuel cells (MFCs) based on Escherichia coli (ATCC 25922). The electrochemical activities of plain stainless steel mesh (SSM), polytetrauoroethylene (PTFE) modified SSM (PMS) and graphene modified SSM (GMS) have been investigated by cyclic voltammetry (CV), discharge experiment and polarization curve measurement. The GMS shows better electrochemical performance than those of SSM and PMS. The MFC equipped with GMS anode delivers a maximum power density of 2668 mW m, which is 18 times larger than that obtained from the MFC with the SSM anode and is 17 times larger than that obtained from the MFC with the PMS anode. Scanning electron microscopy (SEM) results indicate that the increase in power generation could be attributed to the high surface area of anode and an increase in the number of bacteria attached to anode

DataSheet_1_Single-cell transcriptome analysis reveals heterogeneity and convergence of the tumor microenvironment in colorectal cancer.zip

IntroductionColorectal cancer (CRC) ranks second for mortality and third for morbidity among the most commonly diagnosed cancers worldwide. We aimed to investigate the heterogeneity and convergence of tumor microenvironment (TME) in CRC.MethodsWe analyzed the single-cell RNA sequencing data obtained from the Gene Expression Omnibus (GEO) database and identified 8 major cell types and 25 subgroups derived from tumor, para-tumor and peripheral blood.ResultsIn this study, we found that there were significant differences in metabolic patterns, immunophenotypes and transcription factor (TF) regulatory patterns among different subgroups of each major cell type. However, subgroups manifested similar lipid metabolic patterns, immunosuppressive functions and TFs module at the end of the differentiation trajectory in CD8+ T cells, myeloid cells and Fibroblasts. Meanwhile, TFs regulated lipid metabolism and immunosuppressive ligand-receptor pairs were detected by tracing the differentiation trajectory. Based on the cell subgroup fractions calculated by CIBERSORTx and bulk RNA-sequencing data from The Cancer Genome Atlas (TCGA), we constructed an immune risk model and clinical risk model of CRC which presented excellent prognostic value.ConclusionThis study identified that the differentiation was accompanied by remodeling of lipid metabolism and suppression of immune function, which suggest that lipid remodeling may be an important trigger of immunosuppression. More importantly, our work provides a new perspective for understanding the heterogeneity and convergence of the TME and will aid the development of prognosis and immunotherapies of CRC patients.</p

High-Voltage Flexible Microsupercapacitors Based on Laser-Induced Graphene

High-voltage energy-storage devices are quite commonly needed for robots and dielectric elastomers. This paper presents a flexible high-voltage microsupercapacitor (MSC) with a planar in-series architecture for the first time based on laser-induced graphene. The high-voltage devices are capable of supplying output voltages ranging from a few to thousands of volts. The measured capacitances for the 1, 3, and 6 V MSCs were 60.5, 20.7, and 10.0 μF, respectively, under an applied current of 1.0 μA. After the 5000-cycle charge–discharge test, the 6 V MSC retained about 97.8% of the initial capacitance. It also was recorded that the all-solid-state 209 V MSC could achieve a high capacitance of 0.43 μF at a low applied current of 0.2 μA and a capacitance of 0.18 μF even at a high applied current of 5.0 μA. We further demonstrate the robust function of our flexible high-voltage MSCs by using them to power a piezoresistive microsensor (6 V) and a walking robot (>2000 V). Considering the simple, direct, and cost-effective fabrication method of our laser-fabricated flexible high-voltage MSCs, this work paves the way and lays the foundation for high-voltage energy-storage devices

High-Voltage Flexible Microsupercapacitors Based on Laser-Induced Graphene

High-voltage energy-storage devices are quite commonly needed for robots and dielectric elastomers. This paper presents a flexible high-voltage microsupercapacitor (MSC) with a planar in-series architecture for the first time based on laser-induced graphene. The high-voltage devices are capable of supplying output voltages ranging from a few to thousands of volts. The measured capacitances for the 1, 3, and 6 V MSCs were 60.5, 20.7, and 10.0 μF, respectively, under an applied current of 1.0 μA. After the 5000-cycle charge–discharge test, the 6 V MSC retained about 97.8% of the initial capacitance. It also was recorded that the all-solid-state 209 V MSC could achieve a high capacitance of 0.43 μF at a low applied current of 0.2 μA and a capacitance of 0.18 μF even at a high applied current of 5.0 μA. We further demonstrate the robust function of our flexible high-voltage MSCs by using them to power a piezoresistive microsensor (6 V) and a walking robot (>2000 V). Considering the simple, direct, and cost-effective fabrication method of our laser-fabricated flexible high-voltage MSCs, this work paves the way and lays the foundation for high-voltage energy-storage devices