Cloning and characterization of two subunits of calcineurin cDNA in naked carp (Gymnocypris przewalskii) from Lake Qinghai, China

The naked carp (Gymnocypris przewalskii), a native teleost, plays an important role in maintenance of the ecological balance in the system of Lake Qinghai (altitude, 3.2 km) on the Qinghai-Tibet Plateau in China. Calcineurin (CN) is the only member of the serine/threonine phosphatase family that can be activated by both Ca2+ and calmodulin (CaM) and involved in many important physiological processes such as salt tolerance/adaption. In this report, cDNAs of CN catalytic subunit paralogue isoforms: GpCAα (GenBank accession no.JQ407043), GpCAγ (GenBank accession no. JQ407043), and CN regulatory subunit (GpCB) (GenBank accession no. JQ410473), were isolated from Gymnocypris przewalskii and their expression patterns in embryos developmentwere characterized. Gene expression profile demonstrated that GpCA and GpCB mRNA was distributed ubiquitously in all embryonic stages and showed decline until final stage of development. Immunohistologicalanalysis revealed CN localization in different tissues including kidney, heart, brain, spermary, and gill. Collectively, these results provide molecular basis and clues to further understand the role of CN during embryos development and its function in tissues for the adaptation mechanism of naked carp

Power optimization control of VSC-HVDC system for electromechanical oscillation suppression and grid frequency control

The voltage source converter (VSC) based high-voltage DC (HVDC) transmission system usually adopts damping and inertia control to quickly and independently adjust the active- and reactive- power, to improve the frequency stability and suppress the electromechanical oscillations of the power grid. This paper first analyzes the effect of the proportional-derivative (PD) controller parameter on the HVDC output power. The study shows that when the proportional-derivative controller parameter is increased to the limit value, HVDC will operate in the rapid power compensation (RPC) mode. Namely, according to the positive or negative polarities of the rotor speed deviation and the grid frequency deviation, the active- and reactive- power limits are used as the reference to rapidly control the output power, thereby minimizing the system’s unbalanced power, the rotor oscillation, and the frequency fluctuation. To this end, this paper proposes a coordinated active-/reactive- power control strategy for the VSC-HVDC system based on the RPC mode to suppress the grid electromechanical and frequency oscillations. The RPC mode enables HVDC to quickly release/absorb power, to compensate for system’s required power shortage or suppress excess power. When the speed deviation, the frequency deviation, and their rates of change meet the requirements, the damping control is used to make HVDC exit the RPC mode and further enhance the ability of the VSC-HVDC system. Simulation results prove the effectiveness of the proposed power optimization control strategy

Genomic insights into local adaptation and future climate-induced vulnerability of a keystone forest tree in East Asia

Assessment of population vulnerability and adaptive capacity under climate change is crucial for informing conservation strategies. Sang et al. assemble a reference genome for Populus koreana and combine population genomics and modelling to predict spatiotemporal responses to climate change.Rapid global climate change is posing a substantial threat to biodiversity. The assessment of population vulnerability and adaptive capacity under climate change is crucial for informing conservation and mitigation strategies. Here we generate a chromosome-scale genome assembly and re-sequence genomes of 230 individuals collected from 24 populations for Populus koreana, a pioneer and keystone tree species in temperate forests of East Asia. We integrate population genomics and environmental variables to reveal a set of climate-associated single-nucleotide polymorphisms, insertion/deletions and structural variations, especially numerous adaptive non-coding variants distributed across the genome. We incorporate these variants into an environmental modeling scheme to predict a highly spatiotemporal shift of this species in response to future climate change. We further identify the most vulnerable populations that need conservation priority and many candidate genes and variants that may be useful for forest tree breeding with special aims. Our findings highlight the importance of integrating genomic and environmental data to predict adaptive capacity of a key forest to rapid climate change in the future

Single Nucleotide Polymorphisms of Toll-Like Receptor 4 Decrease the Risk of Development of Hepatocellular Carcinoma

BACKGROUND: Toll-like receptor 4 (TLR4) is a key innate immunity receptor that initiates an inflammatory response. Growing evidence suggests that mutation of TLR4 gene may play a role in the development of cancers. This study aimed to investigate the temporal relationship of single nucleotide polymorphisms of TLR4 and the risk of hepatocellular carcinoma, a single center-based case-control study was conducted. METHODS: A systematic genetic analysis of sequence variants of TLR4 by evaluating ten single-nucleotide polymorphisms was performed from 216 hepatocellular carcinoma cases and 228 controls. RESULTS: Six single nucleotide polymorphisms of the TLR4 in the 5'-untranslated region and intron were associated with risk of hepatocellular carcinoma. Individuals carrying the heterozygous genotypes for the rs10759930, rs2737190, rs10116253, rs1927914, rs12377632 and rs1927911 had significantly decreased risk of hepatocellular carcinoma (adjusted odds ratio [OR], from 0.527 to 0.578, P<0.01) comparing with those carrying wild-type homozygous genotypes. In haplotype analysis, one haplotype (GCCCTTAG) of TLR4 was associated significantly with decrease of the occurrence of hepatocellular carcinoma (OR, 0.556, 95% confidence interval [CI], 0.407-0.758, P = 0.000). CONCLUSIONS: Collectively, these results suggested that the risk of hepatocellular carcinoma was associated with TLR4 sequence variation. TLR4 single nucleotide polymorphisms may play an important protective role in the development of hepatocellular carcinoma

Single-frequency hybrid Brillouin-thulium fiber laser with kilohertz linewidth

Stimulated Brillouin scattering (SBS) is a well established method to narrow the laser linewidth to kilohertz level, which however suffers high threshold due to the low SBS gain at the region of 2 mu m. The hybrid Brillouin/thulium fiber laser (BTFL) is such an approach which could suppress the laser linewidth with low threshold and high efficiency. In this paper, an ultra-narrow linewidth hybrid Brillouin/thulium fiber laser (BTFL) was demonstrated. Through experimentally optimizing the output coupling, pump scheme, Brillouin pump power and cavity length of the laser, 344-mW laser output with a narrow linewidth of 0.93 kHz was obtained, in which the linewidth of Stokes light was suppressed more than 43 times compared with the 40 kHz linewidth of the Brillouin pump. Besides, the influences of output coupling and pump scheme on the power and linewidth behavior of a single-frequency BTFL were also experimentally investigated, and there exists a performance balance among linewidth narrowing, output power and SBS threshold. The BTFL output power was further boosted to 5.5 W by a one-stage cladding-pumped fiber amplifier, and the corresponding spectral linewidth was broadened to 1.93 kHz. The output coupling exerted a significant influence on the BTFL performance.This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

Suppression strategy of coupled oscillation and power anti‐regulation of variable speed pumped storage unit with full size converter

Abstract The ultra‐low frequency oscillation and poor speed regulation performance caused by the fast power control decrease the stability and efficiency of the variable speed pumped storage unit with a full size converter (FSC‐VSPSU). This paper presents a fast frequency control strategy to solve this problem. The small‐signal model of the FSC‐VSPSU in fast frequency mode is established, and the key parameters for the system stability are studied by the eigenvalue method. Aiming at the coupling oscillation mode caused by the governor and generator side converter (GSC), the corresponding control parameters optimisation method is proposed. In order to solve the power anti‐regulation problem, the strategy of combining rotor kinetic energy release, power slope adjustment, and delay acceleration control is proposed. PSCAD and RTDS simulation results show that the proposed strategy can effectively avoid the ultra‐low frequency oscillation caused by the fast power control and significantly improves the frequency and power control performance

Stability of truncated sampled-data control systems with impulsive effects

This article considers the stability problem of truncated sampled-data control systems with impulsive effects. We propose the concept of average sampling interval (ASI), in which the constraint on the lower/upper bound of sampling intervals is removed to handle the sampling intervals from the holistic perspective. Then, in the framework of the input delay method, we transform the addressed system into an equivalent switched time-delay system subject to the impulsive effects that result from the truncated sampled-data control law. Interestingly, it is shown that the truncation of the sampled-data control input, which is an extension of the classical zero-order hold function, can play a stabilizing role in the presence of impulsive effects. Next, based on the ASI concept, the idea of average truncated length is introduced and the corresponding relaxed stability criteria are derived for the truncated sampled-data control systems. Particularly, the potential destabilizing impact of impulsive effects on the stability is theoretically revealed. It is also shown that under certain conditions, a truncated sampled-data system can remain stable if the magnitude of the impulsive effects is sufficiently small. Finally, two illustrative examples are presented to show the validity and also the advantages of the obtained results

VSC-HVDC control without PLL for unbalanced grid

When unbalanced faults such as single-phase voltage drop occur in the power grid, VSC-HVDC will fail or become unstable due to sudden changes in amplitude, phase, frequency, etc. of the power grid voltage. Therefore, the role of PLL in a non-ideal power grid is particularly important, but often PLL needs to make a compromise between dynamic response and system stability. Therefore, this paper proposes a free-PLL control strategy under non ideal power grid. First, according to the power transmission model of VSC-HVDC under power grid imbalance, the control command required by the inverter is given, which is determined in the environment free-PLL. Therefore, PLL is not required to retrace the phase of the grid voltage. In addition, in order to overcome the impact of the sudden change of power grid frequency on the free-PLL strategy, a frequency locking strategy is proposed in the free-PLL environment, the response time is about 1 grid cycle. Finally, in order to verify the effectiveness and correctness of the proposed strategy, the VSC-HVDC system’s active power transmission stability is guaranteed when the grid voltage encounters different degrees of single-phase sags