Gold nanoparticle-based paper sensor for multiple detection of 12 Listeria spp. by P60-mediated monoclonal antibody

<p>The genus of <i>Listeria</i> consists of heterologous species and its presence in the food chain is an indicator of poor hygiene. However, a portable and simple paper sensor for detection of listeria spp. with high accuracy was still unknown. In this study, we prepared a pair of monoclonal antibodies (mAbs) that specifically recognize the P60 protein on the cell surface of <i>Listeria</i> spp. The selected pair was found to be sensitive to both the P60 protein and cell body of the genus <i>Listeria</i>. On this basis, a rapid paper sensor was established for sensitive <i>Listeria</i> spp. detection. The developed paper sensor broadly cross-reacted with the 12 tested strains of <i>Listeria</i> and the sensitivity in PBS buffer was 10³–10⁴ colony-forming units (CFU) judged by the gray values of the test line. No cross-reaction with any other gram-positive or gram-negative strains tested was observed. A study using milk samples showed that this paper sensor could detect samples contaminated with low levels of the tested <i>Listeria</i> spp. (1–9 CFU/mL) after 8 h of enrichment and further concentrate for approximately 10 times by centrifugation. The results were in accordance with those obtained using the polymerase chain reaction method.</p

Stabilizing Li₁₀SnP₂S₁₂/Li Interface via an in Situ Formed Solid Electrolyte Interphase Layer

Despite the extremely high ionic conductivity, the commercialization of Li₁₀GeP₂S₁₂-type materials is hindered by the poor stability against Li metal. Herein, to address that issue, a simple strategy is proposed and demonstrated for the first time, i.e., in situ modification of the interface between Li metal and Li₁₀SnP₂S₁₂ (LSPS) by pretreatment with specific ionic liquid and salts. X-ray photoelectron spectroscopy and electrochemical impedance spectroscopy results reveal that a stable solid electrolyte interphase (SEI) layer instead of a mixed conducting layer is formed on Li metal by adding 1.5 M lithium bis­(trifluoromethanesulfonyl)­imide (LiTFSI)/<i>N</i>-propyl-<i>N</i>-methyl pyrrolidinium bis­(trifluoromethanesulfonyl)­imide (Pyr₁₃TFSI) ionic liquid, where ionic liquid not only acts as a wetting agent but also improves the stability at the Li/LSPS interface. This stable SEI layer can prevent LSPS from directly contacting the Li metal and further decomposition, and the Li/LSPS/Li symmetric cell with 1.5 M LiTFSI/Pyr₁₃TFSI attains a stable cycle life of over 1000 h with both the charge and discharge voltages reaching about 50 mV at 0.038 mA cm^–2. Furthermore, the effects of different Li salts on the interfacial modification is also compared and investigated. It is shown that lithium bis­(fluorosulfonyl) imide (LiFSI) salt causes the enrichment of LiF in the SEI layer and results in a higher resistance of the cell upon a long cycling life