Cyclins A and B1 gene expression in rat spleens following aniline exposure.

<p>The fold change in mRNA expression (2^−ΔΔCT) was determined by real-time PCR analysis. Values are means ± SD (<i>n</i> = 3). *<i>p</i> < 0.05 vs. respective controls.</p

p27 and p21 gene expression in the rat spleens following aniline exposure.

<p>The fold change in mRNA expression (2^−ΔΔCT) was determined by real-time PCR analysis. Values are means ± SD (<i>n</i> = 3). *<i>p</i> < 0.05 vs. respective controls.</p

Protein expression of Trx-1 and Ref-1 in rat spleens.

<p>(A) Western blot determination of Trx-1 and Ref-1 in the control and aniline-treated rats. (B) Densitometric measurement of the protein bands. Values are mean ± SD; *<i>p</i> < 0.05.</p

Primer sequences of cyclins A, B1, p21 and p27 for real-time PCR analysis.

<p>Primer sequences of cyclins A, B1, p21 and p27 for real-time PCR analysis.</p

Real-time PCR analysis of miRNAs Let-7a, miR-15b, miR24, miR-100 and miR-125 (A), and miRNAs miR-181a, miR-221 and miR-222 (B) expression in rat spleens following aniline exposure.

<p>Values are means ± SD (<i>n</i> = 3). *<i>p</i> < 0.05 vs. respective controls.</p

Protein expression of CDK1 and p-CDK1 in rat spleens.

<p>(A) Western blot determination of CDK1 and p-CDK1 protein expression in control and aniline-treated rats. (B) Densitometric analysis of protein expressions. Values are mean ± SD; *<i>p</i> < 0.05.</p

Target sequences of miRNAs for real-time PCR analysis.

<p>Target sequences of miRNAs for real-time PCR analysis.</p

Summary of nitrated proteins in control, TCE- and TCE+NAC-treated mice.

<p>*Spot numbers are the same as shown in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098660#pone-0098660-g005" target="_blank">Fig.5</a>; ^#The accession number are in NCBI database; ⁺A score >64 was considered significant (p<0.05) for protein identified from the database.</p

The plausible mechanisms of TCE-induced autoimmune response and its attenuation by NAC supplementation.

<p>The plausible mechanisms of TCE-induced autoimmune response and its attenuation by NAC supplementation.</p

Protein expression (A) and phosphorylation (B) of NF-κB p65 in the livers of MRL+/+ mice treated with TCE, NAC or TCE+NAC for 6 weeks.

<p>The values are means ± SD. *p<0.05 vs. controls; ^#p<0.05 vs. TCE-treated mice. β-actin was used as loading control.</p