The clinical data in 8 cases of benign orbital tumors with bone destructions in children.

<p>The clinical data in 8 cases of benign orbital tumors with bone destructions in children.</p

A–C: Patient with orbital eosinophilic granuloma (case4).

<p>Figure 1A: Clinical appearance of fullness of the upper eyelid of the right eye. Figure 1B: Computed tomography (CT) shows erosion of an intraorbital soft tissue mass through anterior and posterior cortex of frontal bone, similar to malignant tumors. Figure 1C: The tumor tissues comprised pathologic Langerhans cells, eosinophils, scat-tered lymphocytes, plasma cells, and multinucleated giant cells (magnification ×400; hematoxylin-eosin stain).</p

Histopathological involvements using the International Classification for Retinoblastoma (ICRB).

<p>Histopathological involvements using the International Classification for Retinoblastoma (ICRB).</p

A–E: Patient with orbital leiomyoma(case7).

<p>Figure 2A: Clinical appearance of a hard, un-movable, well-marginated mass measuring 15 mm×10 mm in the left temporal periorbital area. Figure 2B, C: Computed tomography (Axial, Figure 2B; Coronal, Figure 2C) revealed a 22 mm×13 mm well-defined soft tissue mass. There was marked destruction of the lateral orbital wall. Figure 2D: The histopathologic examination showed that the tumor composed of spindle-shaped, benign-appearing cells organized in fascicles or loosely arranged in a myxoid stroma (magnification ×200; hematoxylin-eosin stain). Figure 2E: In immunohistochemical staining, the specimen was positive for alpha-smooth muscle actin (magnification ×200).</p

Histopathological involvements of retinoblastoma using the Reese-Ellsworth Staging systems.

<p>Postlaminar 1: postlaminar without invasion of cut end of optic nerve;</p><p>Postlaminar 2: postlaminar with invasion of cut end of optic nerve.</p

Photograph of the fundus showing a pink-white tumor of the right retina in a 2-year-old child, classified as Reese-Ellsworth classification II and International Classification for Retinoblastoma Group C.

<p>Photograph of the fundus showing a pink-white tumor of the right retina in a 2-year-old child, classified as Reese-Ellsworth classification II and International Classification for Retinoblastoma Group C.</p

Histopathology slides showing postlaminar optic nerve invasion of the tumor cells (hematoxylin-eosin stain).

<p>Histopathology slides showing postlaminar optic nerve invasion of the tumor cells (hematoxylin-eosin stain).</p

A–D: Patient with primary orbital intraosseous hemangioma complicated with hematoma (case8).

<p>Figure 3A: Clinical appearance of lower eyelid mass with obvious upward displacement of the right eye. Figure 3B, C: Computed tomography scan (Axial, Figure 3B; Sagittal, Figure 3C) disclosed a smoothly outlined homogeneous soft tissue mass in the inferior-anterior part of the right orbit, with remarkable bone destruction of the lower orbital rim. Figure 3D: The histopathologic finding of primary orbital intraosseous hemangioma, consisting of the thin-walled blood vessels which are closely clustered and separated by normal bony tissue (magnification ×200; hematoxylin-eosin stain).</p

Western blot analysis of Hsp3101, Hsp3102, Hsp3105 and Sdj1 in wild-type, Δ<i>sty1</i> and Δ<i>atf1</i> cells.

<p>Wild-type, Δ<i>sty1</i> and Δ<i>atf1</i> cells were grown in YES medium. Cell lysates were prepared, and proteins were separated by SDS/PAGE and analyzed by immunoblotting with indicated antibodies. Sla1 serves as the loading control.</p

Induction of <i>S</i>. <i>pombe</i> homologs of DJ-1.

<p>(A) qRT-PCR analysis of expression of <i>S</i>. <i>pombe hsp3101-hsp3105</i> and <i>sdj1</i> genes in the wild-type cells. Total RNA was isolated from the wild-type cells grown in YES medium at the indicated time points. All mRNA levels were normalized to the control <i>act1</i>⁺ mRNA level and were expressed as fold change relative to the mRNA levels at the 7 h time point, which was set at a value of 1. Data are presented as mean ± SD (<i>p</i> ≤0.01; <i>t</i> test). (B) Immunoblot analyses of Hsp3101, Hsp3102, Hsp3105 and Sdj1 expression in wild-type cells. Crude extracts were prepared from the wild-type cells at indicated time points (h). Total proteins were separated on SDS/PAGE gels and immunoblotted using anti-Hsp3101 Ab, anti- Sdj1 Ab, anti-Myc Ab, which detects Hsp3102-Myc and Hsp3105-Myc, and anti-Sla1 Ab (serves as a loading control).</p