MOESM1 of MiR-1-3p that correlates with left ventricular function of HCM can serve as a potential target and differentiate HCM from DCM

Additional file

Additional file 3: of Genome-wide identification, characterization, and evolutionary analysis of flowering genes in radish (Raphanus sativus L.)

Flowering genes identified in Raphanus sativus, Brassica oleracea, and Brassica rapa. (XLS 64 kb

Additional file 1 of Gut microbiota-bile acid crosstalk regulates murine lipid metabolism via the intestinal FXR-FGF19 axis in diet-induced humanized dyslipidemia

Additional file 1: Figure S1. FMT from dyslipidemic donors (FMT-dd) can’t induce dyslipidemia in rats. But FMT-dd combining with high-fat diet (HD) disrupted lipid homeostasis and altered gut microbiota in rats. Figure S2. FMT-dd disrupted lipid homeostasis in mice independently, and HD increased the symptoms. Figure S3. Antibiotic pretreatment aggravated the FMT-dd induced dyslipidemia and affect the colonization of human gut microbiota in mice. Figure S4. FMT caused abnormal lipid metabolic pathways in mice. Figure S5. FMT-dd induced dyslipidemia under HD by regulating bile acid synthesis via the hepatic FXR-SHP axis under normal diet and bile acid absorption via the intestine FXR-FGF19 axis under high-fat diet. Table S1. The composition of normal diet and high-fat diet. Table S2. Donors’ information in this study. Table S3. Bile acid retention times, precursor and collision energy used in UPLC-MS/MS methodology. Table S4. Primer sequences used for qPCR

Transcriptome Analysis of <i>Barbarea vulgaris</i> Infested with Diamondback Moth (<i>Plutella xylostella</i>) Larvae

<div><p>Background</p><p>The diamondback moth (DBM, <i>Plutella xylostella</i>) is a crucifer-specific pest that causes significant crop losses worldwide. <i>Barbarea vulgaris</i> (Brassicaceae) can resist DBM and other herbivorous insects by producing feeding-deterrent triterpenoid saponins. Plant breeders have long aimed to transfer this insect resistance to other crops. However, a lack of knowledge on the biosynthetic pathways and regulatory networks of these insecticidal saponins has hindered their practical application. A pyrosequencing-based transcriptome analysis of <i>B. vulgaris</i> during DBM larval feeding was performed to identify genes and gene networks responsible for saponin biosynthesis and its regulation at the genome level.</p><p>Principal Findings</p><p>Approximately 1.22, 1.19, 1.16, 1.23, 1.16, 1.20, and 2.39 giga base pairs of clean nucleotides were generated from <i>B. vulgaris</i> transcriptomes sampled 1, 4, 8, 12, 24, and 48 h after onset of <i>P. xylostella</i> feeding and from non-inoculated controls, respectively. <i>De novo</i> assembly using all data of the seven transcriptomes generated 39,531 unigenes. A total of 37,780 (95.57%) unigenes were annotated, 14,399 of which were assigned to one or more gene ontology terms and 19,620 of which were assigned to 126 known pathways. Expression profiles revealed 2,016–4,685 up-regulated and 557–5188 down-regulated transcripts. Secondary metabolic pathways, such as those of terpenoids, glucosinolates, and phenylpropanoids, and its related regulators were elevated. Candidate genes for the triterpene saponin pathway were found in the transcriptome. Orthological analysis of the transcriptome with four other crucifer transcriptomes identified 592 <i>B. vulgaris</i>-specific gene families with a <i>P</i>-value cutoff of 1e⁻⁵.</p><p>Conclusion</p><p>This study presents the first comprehensive transcriptome analysis of <i>B. vulgaris</i> subjected to a series of DBM feedings. The biosynthetic and regulatory pathways of triterpenoid saponins and other DBM deterrent metabolites in this plant were classified. The results of this study will provide useful data for future investigations on pest-resistance phytochemistry and plant breeding.</p></div

Histoire complète et seule véritable du petit bossu Mayeux, chasseur de la garde nationale, écrite par lui-même et suivie de sa chanson

Avec mode text

Characterization of TTN Novex Splicing Variants across Species and the Role of RBM20 in Novex-Specific Exon Splicing

<p> Titin ( TTN) is a major disease-causing gene in cardiac muscle. Titin ( TTN) contains 363 exons</p> <p>in human encoding various sizes of TTN protein due to alternative splicing regulated mainly by</p> <p>RNA binding motif 20 (RBM20). Three isoforms of TTN protein are produced by mutually exclusive</p> <p>exons 45 (Novex 1), 46 (Novex 2), and 48 (Novex 3). Alternatively splicing in Novex isoforms</p> <p>across species and whether Novex isoforms are associated with heart disease remains completely</p> <p>unknown. Cross-species exon comparison with the mVISTA online tool revealed that exon 45 is</p> <p>more highly conserved across all species than exons 46 and 48. Importantly, a conserved region</p> <p>between exons 47 and 48 across species was revealed for the first time. Reverse transcript polymerase</p> <p>chain reaction (RT-PCR) and DNA sequencing confirmed a new exon named as 480 in Novex 3.</p> <p>In addition, with primer pairs for Novex 1, a new truncated form preserving introns 44 and 45 was</p> <p>discovered. We discovered that Novex 2 is not expressed in the pig, mouse, and rat with Novex 2</p> <p>primer pairs. Unexpectedly, three truncated forms were identified. One TTN variant with intron 46</p> <p>retention is mainly expressed in the human and frog heart, another variant with co-expression of</p> <p>exons 45 and 46 exists predominantly in chicken and frog heart, and a third with retention of introns</p> <p>45 and 46 is mainly expressed in pig, mouse, rat, and chicken. Using Rbm20 knockout rat heart,</p> <p>we revealed that RBM20 is not a splicing regulator of Novex variants. Furthermore, the expression</p> <p>levels of Novex variants in human hearts with cardiomyopathies suggested that Novexes 2 and 3</p> <p>could be associated with dilated cardiomyopathy (DCM) and/or arrhythmogenic right ventricular</p> <p>cardiomyopathy (ARVC). Taken together, our study reveals that splicing diversity of Novex exons</p> <p>across species and Novex variants might play a role in cardiomyopathy.</p

Additional file 1 of Single-cell RNA sequencing in donor and end-stage heart failure patients identifies NLRP3 as a therapeutic target for arrhythmogenic right ventricular cardiomyopathy

Additional file 1: Table S1. Clinical information of ARVC patients based on Task Force Criteria in 2010. Table S2. Clinical characteristics of enrolled ARVC patients and normal controls. Table S3. Counts of different biotypes. Table S4. Cell types assignment by using SingleR and manual annotation. Table S5. Current list of GWAS cardiac arrhythmia genes. Table S6. The summary of major non-cardiomyocytes subpopulations in ARVC and normal human hearts