Independent variables and their correspondent values for the optimization of SPC preparation, using the central composite design.

<p>Independent variables and their correspondent values for the optimization of SPC preparation, using the central composite design.</p

TEM graphs of conventional SEDDS (A) and Super-SEDDS (B).

<p>TEM graphs of conventional SEDDS (A) and Super-SEDDS (B).</p

Response surface plots.

<p>The significant (<i>p</i><0.05) interaction effects for combination percentage, as a function of X1/X2 and X1/X3, are presented as 3D surfaces (A, C) and contours (B, D) respectively. X1: the ratio of scutellarin to phospholipid; X2: the temperature of the water bath; X3: the drug concentration in solvent.</p

The plasma concentration-time curves of scutellarin.

<p>Scutellarin powder (black), SPC (blue), conventional SEDDS (green) and Super-SEDDS (red) were administrated to each group of SD rats (n = 5) respectively, with the dose of 40 mg/kg calculated in scutellarin. Blood samples were collected prior to the dose and at 0.5, 1, 2, 3, 4, 5, 6, 8, 10, 12, 24, and 36 h after the dose.</p

The <i>in vitro</i> lipolysis profiles.

<p>Lipolysis release properties of scutellarin from scutellarin powder (black), SPC (blue), conventional SEDDS (green) and Super-SEDDS (red), evaluated as cumulative dissolution percentages, were investigated in lipolysis medium (pH 6.5, with 2000 TBU/mL pancreatic lipase) at 37°C (n = 3).</p

Lipophilicify evaluation and solubility study of scutellarin powder and SPC.

<p>Octanol-water distribution coefficients of scutellarin powder (black line) and SPC (blue line) were measured in the range of pH 2∼8 (A). Solubilities of scutellarin powder (black column) and SPC (blue column) in different vehicles were compared. Each test was performed 3 times in parallel (B).</p

Analysis of variance (ANOVA) of regression coefficients using F-value and <i>p</i>-value.

<p>Where, Xi, XiXj and Xi² are the linear and interaction terms of the quadratic polynomial equation, respectively. The significant (<i>p</i><0.05) terms are labeled with *.</p

The validation of analytical methodology including accuracy and precision from quality control samples of rat plasma extracts (n = 3 days, five replicates per day).

<p>The validation of analytical methodology including accuracy and precision from quality control samples of rat plasma extracts (n = 3 days, five replicates per day).</p

X-ray diffraction patterns of phospholipid, physical mixture, SPC and scutellarin powder.

<p>All samples were scanned over a range of 2 θ angles from 3°C to 65°C with an angular increment of 0.02°C per second.</p

The structure of scutellarin.

<p>The structure of scutellarin.</p