RMDAP: A Versatile, Ready-To-Use Toolbox for Multigene Genetic Transformation

Background: The use of transgenes to improve complex traits in crops has challenged current genetic transformation technology for multigene transfer. Therefore, a multigene transformation strategy for use in plant molecular biology and plant genetic breeding is thus needed. Methodology/Principal Findings: Here we describe a versatile, ready-to-use multigene genetic transformation method, named the Recombination-assisted Multifunctional DNA Assembly Platform (RMDAP), which combines many of the useful features of existing plant transformation systems. This platform incorporates three widely-used recombination systems, namely, Gateway technology, in vivo Cre/loxP and recombineering into a highly efficient and reliable approach for gene assembly. RMDAP proposes a strategy for gene stacking and contains a wide range of flexible, modular vectors offering a series of functionally validated genetic elements to manipulate transgene overexpression or gene silencing involved in a metabolic pathway. In particular, the ability to construct a multigene marker-free vector is another attractive feature. The built-in flexibility of original vectors has greatly increased the expansibility and applicability of the system. A proof-ofprinciple experiment was confirmed by successfully transferring several heterologous genes into the plant genome. Conclusions/Significance: This platform is a ready-to-use toolbox for full exploitation of the potential for coordinate regulation of metabolic pathways and molecular breeding, and will eventually achieve the aim of what we call ‘‘one-sto

Generation of (3, 1) Vector Signal Based on Probabilistic Shaping Technology without Precoding

In this paper, we introduce the probabilistic shaping (PS) technique to the normal (3, 1) vector signal and simulate the generated PS (3, 1) photonic vector signal on an optical transmission system. The PS (3, 1) photonic vector signal is generated by a radio frequency (RF) signal at 12 GHz driving a Mach–Zehnder modulator- (MZM-) based optical carrier suppression (OCS) doubling, and the PS (3, 1) photonic vector signal is not precoding. The PS (3, 1) photonic vector signal and the normal (3, 1) photonic vector signal are used to transmit in 5 km, 10 km, and 20 km single-mode fibers (SMF), respectively. The simulation results demonstrate that the bit error ratio (BER) of the PS (3, 1) vector signal is less than the forward error correction (FEC) threshold of 3.8 × 10−3, and the BER performance is better than that of the normal (3, 1) vector signal at 4 Gbit/s and 8 Gbit/s transmission rates

Review on the Millimeter-Wave Generation Techniques Based on Photon Assisted for the RoF Network System

With the development trend of wireless and broadband in the communication link and even the whole information industry, the demand of high-frequency microwave bandwidth has been increasing. The RoF network system solves the problem of spectrum congestion in low-frequency band by providing an effective technology for the distribution of high-frequency microwave signals over optical fiber links. However, the traditional mm-wave generation technique is limited by the bandwidth of electronic devices. It is difficult to generate high-frequency and low-phase noise mm-wave signals with pure electrical components. The mm-wave communication technology based on photon assisted can overcome the bandwidth bottleneck of electronic devices and provide the potential for developing the low-cost infrastructure demand of broadband mobile services. This paper will briefly explain the characteristics of the RoF network system and the advantages of high-frequency mm-wave. Then we, respectively, introduce the modulation schemes of RoF mm-wave generation based on photon assisted including directly modulated laser (DML), external modulation, and optical heterodyne. The review mainly focuses on a variety of different mm-wave generation technologies including multifrequency vector mm-wave. Furthermore, we list several approaches to realize the large capacity data transmission techniques and describe the digital signal processing (DSP) algorithm flow in the receiver. In the end, we summarize the RoF network system and look forward to the future

Copper/functionalized-carbon nanotubes composite films with ultrahigh electrical conductivity prepared by pulse reverse electrodeposition

Carbon nanotubes (CNTs) have been proved a significant role as the reinforcement material in improving the mechanical and electrical properties of metal matrix composites due to their high mechanical properties, excellent electrical and thermal conductivity as well as unique atomic structure. In addition, the dispersion of CNTs has been a key factor in fabricating of metal-based complex especially for copper (Cu) with performance improvement. In the present paper, the well dispersion of functionalized CNTs (F-CNTs) is obtained at the first time, accompanied by using pulse reverse electrodeposition (PRED) technology, leading to formation of the ultrahigh electrical conductivity composite films of Cu/F-CNTs. These composite films exhibit an ultrahigh electrical conductivity of up to 6.1 x 10(7)S/m (increased by 105.4% of that international annealed copper standard, IACS), but maintain a high hardness of 82.3 HV and tensile strength of 297.1 MPa. It is believed that this work opens new perspectives to develop ultrahigh electrical conductivity composite materials and would role as electric wire for reducing energy loss