7 research outputs found
Attraction of Spiral Waves by Localized Inhomogeneities with Small-World Connections in Excitable Media
Trapping and un-trapping of spiral tips in a two-dimensional homogeneous
excitable medium with local small-world connections is studied by numerical
simulation. In a homogeneous medium which can be simulated with a lattice of
regular neighborhood connections, the spiral wave is in the meandering regime.
When changing the topology of a small region from regular connections to
small-world connections, the tip of a spiral waves is attracted by the
small-world region, where the average path length declines with the
introduction of long distant connections. The "trapped" phenomenon also occurs
in regular lattices where the diffusion coefficient of the small region is
increased. The above results can be explained by the eikonal equation and the
relation between core radius and diffusion coefficient.Comment: 5 pages, 4 figure
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Adiabatic dynamics of low-lying collective modes in the BEC-BCS crossover
textAs the hydrodynamic theory breaks down with the local density ap-
proximation in the fermionic superļ¬uid with spin-polarization, we develop a
general formalism of the adiabatic dynamics for the low-lying collective modes
in the BEC-BCS crossover, which is exact in the adiabatic limit. This adi-
abatic dynamic theory is based on a static density functional theory of the
spin-polarized superļ¬uid system, which we derive as a generalization of the
conventional density functional theory of superļ¬uid for current experimental
interests. A special case where the system is uniform and analytically solv-
able is studied in detail. We show that our adiabatic equations of motion are
reduced to the hydrodynamic equations of motion within local density approx-
imation, which provides a solid microscopic foundation for the well-publicized
phenomenological hydrodynamic theory.Physic
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Unlocking the Potential of Carbonaceous Resource Recovery from the Arrested Anaerobic Digestion of Food Waste: Engineering Design and Meta-omics Analysis
Organic waste-fueled carbonaceous resource recovery using approaches such as arrested anaerobic digestion generates economically attractive products such as volatile fatty acid (VFA). The production of VFA expands the applications of anaerobic biotechnologies beyond the traditionally produced biogas. Compared to biogas, VFA is produced and recovered in a concentrated form in the aqueous phase, which is more conducive to direct utilization in downstream bioplastic, biodiesel production, and nitrogen/phosphorus removal in water resource-recovery facilities. However, this application is limited by the variability in VFA yield and composition as obtained from different complex solids streams. Additionally, the lack of understanding of the nexus between the performance-structure-function of the microbial community within the arrested anaerobic digestion process leads to the massive gap between the optimized engineering regulations and the high-throughput VFA production.
Consequently, this dissertation aimed to unlock the potential of VFA production with maximized yield and regulated composition through the manipulation of the operational parameter (hydraulic retention time (HRT)) and the feedstock condition (thermal hydrolysis pretreatment (THP)). In response, meta-omics-derived approaches were applied to elucidate the dynamic changes of microbial structure, potential, and extant functionality in terms of the two processes (hydrolysis and acidification) within arrested anaerobic digestion of food waste. Specifically, the objectives were (1) Performance: Evaluate the hydrolysis and acidification performance changes including hydrolysis yield, VFA yield, VFA composition, methane yield, etc. under different HRTs and feeding THP or non-THP food waste. (2) Microbial structure: characterize and compare the significance of HRT and feedstock condition in shaping microbial structures. (3) Functional analysis: Interpret the community-level dynamic changes of potential and extant functions within the (3.1) customized acidification metabolic networks and the (3.2) carbohydrate hydrolysis niches.
The highlighted findings are as follows:
(1) Performance of the arrested anaerobic digestion (including hydrolysis and acidification processes): Neither the hydrolysis yield nor the VFA yield was improved by the extended HRT from 4 to 8 days (P > .05). The inclusion of THP on feedstock didnāt improve the hydrolysis yield (P > .05) while the VFA yield was significantly decreased (P = .003). Among all conditions, the methane production was less than 5% of the chemical oxygen demand (COD) and a propionic acid-dominant type product was robustly formed.
(2) Microbial structures in the arrested anaerobic digestors (including core hydrolyzers and acidification microbial communities): Both HRT and the inclusion of THP on feedstock shaped distinct microbial structures in the arrested anaerobic digestors (P = .02 and .01). Although the extension of HRT didnāt change the Shannon diversity Index (P > .05), it was significantly decreased after feeding with THP food waste (P = .03), which might stem from the reduced indigenous microbes in the initial food waste feedstock. Prevotella was always the most abundant genus under all conditions, which might contribute to the dominantly produced propionic acid among all conditions. The successfully suppressed growth of methanogenic archaea was reflected in terms of the low relative abundance (<1.5%) among all conditions.
(3.1) Functional analysis of the customized acidification metabolic networks: Under the two selected HRTs, the potential and extant functions of acidification were unchanged between the two reactors (P > .05), which indicated a community-level redundancy in convergent potential and extant acidification functions even under a completely shifted microbial structure. However, the inclusion of THP diminished the potential and extant functions of acidification, in the meantime, shifting the main producer of butyric acid from Bacteroides to Prevotella through the expression of gene buk2. Among all conditions, the highest potential and extant functions in propionic acid production corresponded to the propionic acid-dominant acid profile in all reactors. The prevalently enriched Prevotella contributed to the stable propionic acid-dominant production via the acryloyl-CoA to propionyl phosphate to the propionic acid pathway.
(3.2) Functional analysis of the carbohydrate hydrolysis niches: The extension of HRT from 4 days to 8 days didnāt impact the potential and extant functions of carbohydrate-activated enzymes (CAZys) and the hydrolysis of polysaccharides. Only two intermediate steps (gene malQ and lplD) during the hydrolysis of starch and pectin were enhanced with higher absolute transcriptional activities (mRNA/DNA RPKM) under HRT 8 days. The abundance ratio of the two main hydrolysis phyla Firmicutes: Bacteroidetes was unchanged between the two HRTs. When feeding with THP feedstock, the potential and extant functions of CAZys were both enhanced. All steps within the hydrolysis of cellulose (polysaccharides) exhibited increased absolute transcriptional activities (mRNA/DNA RPKM). The abundance ratio of Firmicutes: Bacteroidetes was decreased after the inclusion of THP on feedstock, which corresponded to the increased hydrolysis of polysaccharides- cellulose. Although the carbohydrate hydrolysis functions were improved after feeding with THP food waste, the total hydrolysis yield was not enhanced. The hydrolysis of other compounds such as proteins and lipids could also contribute to the total hydrolysis yield. The taxonomic analysis revealed that in all four conditions, the genus Prevotella presented with the highest potential functions in CAZys, while the genus Pararhodospirillum exhibited the highest extant functions in CAZys. This indicated that distinct bacteria were endowed with different functional potentials of CAZys and mobilized these functions differently.
Overall, this research provides practical suggestions for engineering designs to maximize the VFA production profits from arrested anaerobic digestion of food waste: (1) A properly controlled HRT enables a long-term high-throughput production of VFA with stable yield and the unchanged dominant acid type (2) The inclusion of THP to the feedstock was not suggested to be applied to maximize the VFA yield even the dominant acid type may not change. (3) The dominantly produced propionic acid could be targeted by enriching the Prevotella genus to produce the propionic acid through the acryloyl-CoA to propionyl phosphate to the propionic acid pathway.
Besides the engineering aspect, this research also specifically elucidates the long-time lumped and simplified acidification and carbohydrate hydrolysis processes with the extended metabolic databases including each reaction, key intermediates, enzymes, and corresponding genes. This expanded database served as an essential upstream process, which could be integrated into the current anaerobic digestion model. Additional applications could be extended to the human digestion systems' microbiome and be exploited commercially for other mixed-culture biosynthesis processes such as bioplastic and biodiesel production.
Finally, the application of meta-omics-derived methodology revealed the functional redundancy and the potential discrepancy between the most abundant group and the most actively functional group underlying the formed black box of VFA production performance. This discussion of the nexus of performance-structure-function suggested the importance of applying meta-omics approaches in engineering practice, especially when feeding the mixed-culture community with real complex solid streams. The targeted VFA profiles cannot be reached without identifying the actual functional bacteria under selected engineering conditions
Distortional Buckling Analysis of Steel-Concrete Composite Girders in Negative Moment Area
Distortional buckling is one of the most important buckling modes of the steel-concrete composite girder under negative moment. In this study, the equivalent lateral and torsional restraints of the bottom flange of a steel-concrete composite girder under negative moments due to variable axial forces are thoroughly investigated. The results show that there is a coupling effect between the applied forces and the lateral and torsional restraint of the bottom flange. Based on the calculation formula of lateral and torsional restraints, the critical buckling stress of I-steel-concrete composite girders and steel-concrete composite box girders under variable axial force is obtained. The critical bending moment of the steel-concrete composite girders can be further calculated. Compared to the traditional calculation methods of elastic foundation beam, the paper introduces an improved method, which considers coupling effect of the external loads and the foundation spring constraints of the bottom flange. Fifteen examples of the steel-concrete composite girders in different conditions are calculated. The calculation results show a good match between the hand calculation and the ANSYS finite element method, which validated that the analytic calculation method proposed in this paper is practical
Transcriptomic Analysis of Spleen Revealed Mechanism of Dexamethasone-Induced Immune Suppression in Chicks
Stress-induced immunosuppression is a common problem in the poultry industry, but the specific mechanism of its effect on the immune function of chicken has not been clarified. In this study, 7-day-old Gushi cocks were selected as subjects, and a stress-induced immunosuppression model was successfully established via daily injection of 2.0 mg/kg (body weight) dexamethasone. We characterized the spleen transcriptome in the control (B_S) and model (D_S) groups, and 515 significant differentially expressed genes (SDEGs) (Fragments Per Kilobase of transcript sequence per Millions base pairs sequenced (FPKM) > 1, adjusted p-value (padj) < 0.05 and Fold change (|FC|) ≥ 2) were identified. The cytokine-cytokine receptor interaction signaling pathway was identified as being highly activated during stress-induced immunosuppression, including the following SDEGs—CXCL13L2, CSF3R, CSF2RB, CCR9, CCR10, IL1R1, IL8L1, IL8L2, GHR, KIT, OSMR, TNFRSF13B, TNFSF13B, and TGFBR2L. At the same time, immune-related SDEGs including CCR9, CCR10, DMB1, TNFRSF13B, TNFRSF13C and TNFSF13B were significantly enriched in the intestinal immune network for the IgA production signaling pathway. The SDEG protein-protein interaction module analysis showed that CXCR5, CCR8L, CCR9, CCR10, IL8L2, IL8L1, TNFSF13B, TNFRSF13B and TNFRSF13C may play an important role in stress-induced immunosuppression. These findings provide a background for further research on stress-induced immunosuppression. Thus, we can better understand the molecular genetic mechanism of chicken stress-induced immunosuppression
Molecular-Level Interfacial Chemistry Regulation of MXene Enables Energy Storage beyond Theoretical Limit
Ti3C2Tx MXene
often suffers from poor lithium storage behaviors due to its electrochemically
unfavorable OH terminations. Herein, we propose molecular-level interfacial
chemistry regulation of Ti3C2Tx MXene with phytic acid (PA) to directly activate its OH terminations.
Through constructing hydrogen bonds (H-bonds) between oxygen atoms
of PA and OH terminations on Ti3C2Tx surface, interfacial charge distribution of Ti3C2Tx has been effectively
regulated, thereby enabling sufficient ion-storage sites and expediting
ion transport kinetics for high-performance energy storage. The results
show that Li ions preferably bind to H-bond acceptors (oxygen atoms
from PA), and the flexibility of H-bonds therefore renders their interactions
with adsorbed Li ions chemically ātunableā, thus alleviating
undesirable localized geometric changes of the OH terminations. Meanwhile
the H-bond-induced microscopic dipoles can act as directional Li-ion
pumps to expedite ion diffusion kinetics with lower energy barrier.
As a result, the as-designed Ti3C2Tx/PA achieves a 2.4-fold capacity enhancement compared
with pristine Ti3C2Tx (even beyond theoretical capacity), superior long-term cyclability
(220.0 mAh gā1 after 2000 cycles at 2.0 A gā1), and broad temperature adaptability (ā20
to 50 Ā°C). This work offers a promising interface engineering
strategy to regulate microenvironments of inherent terminations for
breaking through the energy storage performance of MXenes