Design of amine-functionalized metal-organic frameworks for CO2 separation: the more amine, the better?

A total of 41,825 metal-organic frameworks (MOFs) were computationally screened toward the design of amine-functionalized MOFs for CO2 separation. Both the optimal species and number of amine functional groups were examined for eight MOFs with good performance in terms of CO2 uptake and selectivity. It was revealed that more amine functional groups grafted on the MOFs do not lead to a better CO2 separation capability, and the concept of saturation degree of functional groups was proposed. The ethylene-diamine-functionalized MOF-74 membrane was predicted to possess high CO2 permeation separation capability, which was confirmed by the parallel experimental test of gas permeation.National Key Basic Research Program of China/2013CB73350National Natural Science Foundation of China/21376089National Natural Science Foundation of China/91334202China Postdoctoral Science Foundation/2014M560663Guangdong Science Foundation/2014A03031200Guangdong Science Foundation/2014A03031026Fundamental Research Funds for the Central Universities/SCUT-2014ZB0012Fundamental Research Funds for the Central Universities/2015ZP03State Key Lab of Pulp and Paper Engineering Program/201444National University of Singapore for CENGa

Non-uniform changes in membrane receptors in the rat urinary bladder following outlet obstruction.

The aim of the present study was to investigate the expression and distribution of membrane receptors after bladder outlet obstruction (BOO). Partial bladder outlet obstruction (BOO) was induced in female rats and bladders were harvested after either 10 days or 6 weeks of BOO. The expression of different receptors was surveyed by microarrays and corroborated by immunohistochemistry and western blotting. A microarray experiment identified 10 membrane receptors that were differentially expressed compared to sham-operated rats including both upregulated and downregulated receptors. Four of these were selected for functional experiments on the basis of magnitude of change and relevance to bladder physiology. At 6 weeks of BOO, maximal contraction was reduced for neuromedin B and vasopressin (AVP), consistent with reductions of receptor mRNA levels. Glycine receptor-induced contraction on the other hand was increased and receptor mRNA expression was accordingly upregulated. Maximal relaxation by the β3-adrenergic receptor agonist CL316243 was reduced as was the receptor mRNA level. Immunohistochemistry supported reduced expression of neuromedin B receptors, V1a receptors and β3-adrenergic receptors, but glycine receptor expression appeared unchanged. Western blotting confirmed repression of V1a receptors and induction of glycine receptors in BOO. mRNA for vasopressin was detectable in the bladder, suggesting local AVP production. We conclude that changes in receptor expression following bladder outlet obstruction are non-uniform. Some receptors are upregulated, conferring increased responsiveness to agonist, whereas others are downregulated, leading to decreased agonist-induced responses. This study might help to select pharmacological agents that are effective in modulating lower urinary tract symptoms in BOO

Dynamic resilient modulus of subgrade silty clay for heavy-haul railway: an experimental investigation and the predicted method

A large-scale series of cyclic triaxial tests were conducted to explore the evolution of the dynamic resilient modulus of silty clay for the heavy-haul railway subgrade. A novel loading sequence for measuring the dynamic resilient modulus was established, which characterized the dynamic stress state of the subgrade induced by the heavy-haul train load. In the experimental investigation, the deviatoric stresses, confining stress, initial moisture content, and compaction degree were considered as variables, and the effects of the aforementioned variables were evaluated quantitatively. The experimental results showed that the dynamic resilient modulus was negatively related to deviatoric stresses and initial moisture content, where the average decreased rates were 14.65% and 27.79% with the increase in deviatoric stresses from 60 kPa to 150 kPa and increase in the initial moisture content from 9.8% to 15.8%, respectively. Furthermore, the dynamic resilient modulus was positively related to confining stress and compaction degree, where the average increased rates were 23.25% and 27.48% with the increase in confining stress from 20 kPa to 60 kPa and increase in compaction degree from 0.91 to 0.95. To provide a better application, the two high-accuracy predicted methods were established through the empirical model and artificial neural network approach including the aforementioned variables. This study can provide useful guidelines for the effective and safe design of the heavy-haul railway subgrade filled with silty clay

Age-associated changes in synaptic lipid raft proteins revealed by two-dimensional fluorescence difference gel electrophoresis

Brain aging is associated with a progressive decline in cognitive function though the molecular mechanisms remain unknown. Functional changes in brain neurons could be due to age-related alterations in levels of specific proteins critical for information processing. Specialized membrane microdomains known as ‘lipid rafts’ contain protein complexes involved in many signal transduction processes. This study was undertaken to determine if two-dimensional fluorescence difference gel electrophoresis (2D DIGE) analysis of proteins in synaptic membrane lipid rafts revealed age-dependent alterations in levels of raft proteins. Five pairs of young and aged rat synaptic membrane rafts were subjected to DIGE separation, followed by image analysis and identification of significantly altered proteins. Of 1046 matched spots on DIGE gels, 94 showed statistically significant differences in levels between old and young rafts, and 87 of these were decreased in aged rafts. The 41 most significantly altered (p < 0.03) proteins included several synaptic proteins involved in energy metabolism, redox homeostasis, and cytoskeletal structure. This may indicate a disruption in bioenergetic balance and redox homeostasis in synaptic rafts with brain aging. Differential levels of representative identified proteins were confirmed by immunoblot analysis. Our findings provide novel pathways in investigations of mechanisms that may contribute to altered neuronal function in aging brain