Part-Whole Relational Few-Shot 3D Point Cloud Semantic Segmentation

The author wishes to extend sincere appreciation to Professor Lin Shi for the generous provision of equipment support, which significantly aided in the successful completion of this research. Furthermore, the author expresses gratitude to Associate Professor Ning Li and Teacher Wei Guan for their invaluable academic guidance and unwavering support. Their expertise and advice played a crucial role in shaping the direction and quality of this research.Peer reviewe

Experimental study on characterization hydraulic fracturing coal fracture network and evolution of fracture forming performance

The accurate characterization of coal fracture network can effectively evaluate the fracturing effect of the deep coal seam after hydraulic fracturing. In order to quantitatively evaluate the complexity of coal seam after hydraulic fracturing, the hydraulic fracturing test of coal was carried out by using the self-made true triaxial test system, combined with CT scan, the pore network and fracture network with topological structure are reconstructed, and the complexity of the fracture network is quantitatively characterized by fractal theory and topology. The effect of the intermediate principal stress on the complexity of the fracture networks under the condition of true triaxial stress is explored. The results showed that the change rate of the two-dimensional fractal dimension (K) are 1.03% ~ 7.10%, and the change rate of three-dimensional fractal dimension are 3.50% ~ 9.18%, the two-dimensional and three-dimensional fractal dimensions of coal samples after hydraulic fracturing increase significantly. The method based on fractal theory and topology can effectively characterize the fracture structure and fracture forming ability of hydraulic fracturing. The two-dimensional topological parameter of coal samples after fracturing are1.18 ~ 1.52, which is positively correlated with the change rate of two-dimensional fractal dimension．Increasing rate of change rate of two-dimensional fractal dimension decreases with the increase of topological parameters. The reconstructed internal structure and fracture distribution showed that the three-dimensional fractal dimension after fracturing is more advantaged than the two-dimensional fractal dimension. The three-dimensional topological parameters of coal samples after fracturing are 1.82-1.93, increased with the increase of the change rate of three-dimensional fractal dimension. The intermediate principal stress has a positive effect on the seam forming ability of coal seam. The fractal dimension and topological parameters increase with the increase of intermediate principal stress before and after hydraulic fracturing. In other words, Increased with the intermediate principal stress, the resulting fracture network is more complex, the connectivity is better, and the fracture forming ability of hydraulic fracturing is stronger

Comparison of Different Methods for RNA Extraction from Floral Buds of Tree Peony (<i>Paeonia suffruticosa</i> Andr.)

Tree peony (Paeonia suffruticosa Andr.), a species native to China, is one of the most important ornamental and medicinal plants. Like other tree species in temperate and boreal zones, the dormancy-activity transition of floral buds is critical for blooming time and fruit production. However, floral buds contain high levels of secondary metabolites, making the isolation of high quality RNA difficult. To obtain a method suitable for extracting RNA from floral buds of tree peony, we evaluated five different methods, including the cetyltrimethylammonium bromide (CTAB) and Sodium dodecyl sulfate (SDS)-based methods, a modified SDS-TRNzol protocol, and two commercial kits (TRNzol and Qiagen RNeasy Plant Mini Kit). The modified SDS-TRNzol method was capable of efficiently removing polyphenols and other metabolites in floral buds. The isolated RNA was of high purity and integrity, as demonstrated by the the A260/280 ratio of approximately 2.0, and RIN values of more than 9.0. Gel electrophoresis analysis indicated that the extracted RNA had clear 28S and 18S ribosomal RNA bands without DNA contamination. The RNA isolated by this protocol was successfully used for downstream manipulations, such as RT-PCR, RACE, and real-time PCR. Together, the modified SDS-TRNzol protocol is an easy, efficient, and highly reproducible method for RNA isolation from floral buds rich in secondary metabolites

MOESM4 of Stochastic ground-motion simulations for the 2016 Kumamoto, Japan, earthquake

Additional file 4: Figure S4. Comparison of observed and simulated PSAs at all stations

MOESM3 of Stochastic ground-motion simulations for the 2016 Kumamoto, Japan, earthquake

Additional file 3: Figure S3. Comparison of observed and simulated FAS at all stations

MOESM1 of Stochastic ground-motion simulations for the 2016 Kumamoto, Japan, earthquake

Additional file 1: Figure S1. Site amplifications at all stations