Combined effects of tenofovir and interferon α1b on viral load and levels of peripheral regulatory T cells in chronic hepatitis B subjects

Purpose: To study the combined effects of tenofovir and interferon α1b on viral load and peripheral blood regulatory T cell concentrations of chronic hepatitis B (CHB) subjects. Methods: Patients with chronic hepatitis B (86 cases) were randomly assigned to two groups: control group and study group. In control subjects, tenofovir was given orally (300 mg/kg bwt/day). In addition to tenofovir, the study group received interferon α1b injection intramuscularly at a dose of 50 μg/kg thrice a week. Liver function, serum hepatitis B viral (HBV) load, and serum levels of peripheral blood regulatory T-lymphocytes were determined. Clinical effectiveness and adverse reactions in both groups were also assessed. Results: After treatment, total effectiveness was higher in the study group (86.04 %) than in control patients (62.79 %) (p < 0.05). Serum aspartate transaminase (AST), alanine aminotransferase (ALT) and total bilirubin (TBIL) significantly decreased in the study group, relative to control, but HBV DNAnegative, HbeAg-negative and HbsAg-negative cells were markedly higher in patients in the study group (p < 0.05). Moreover, there were higher CD4+ T and CD8+ T counts, and CD4+ T/CD8+ T ratio in study subjects than in control subjects (p < 0.05). Conclusion: The combination of tenofovir with interferon α1b effectively improves liver functions in patients with CHB, reduces viral load, and exerts anti-HBV effect by regulating the levels of peripheral blood T-lymphocytes

Overexpression of ERBB-2 was more frequently detected in malignant than benign pheochromocytomas by multiplex ligation-dependent probe amplification and immunohistochemistry

To analyze the genetic alterations of pheochromocytomas and evaluate the difference among malignant, extra-adrenal, and benign pheochromocytomas. Forty-three tumor samples were tested for genetic changes using multiplex ligation-dependent probe amplification. Among them, 39 samples were available for protein expression analysis by immunohistochemistry (IHC). All 43 patients (24 women and 19 men; mean age 44.6±13.6 years; range 18–75 years; 9 with malignant, 7 extra-adrenal, and 27 benign) showed multiple copy number losses or gains. The average copy number change was 13.10 in malignant, 13.93 in benign, and 13.47 in paraganglioma patients. There is no significant difference among the three groups of pheochromocytomas. However, we discovered that in the malignant pheochromocytomas, 6 of the 9 patients (67%) showed erythroblastic leukemia viral oncogene homolog 2 (ERBB-2) oncogene gain, whereas only 12 of the 34 (35%) identified change in the benign and extra-adrenal pheochromocytomas. Further, IHC confirmed that ERBB-2-positive staining was more frequent and stronger in malignant pheochromocytomas than in benign and extra-adrenal pheochromocytomas. Our study illustrates the chromosomal changes of the whole genome of Chinese pheochromocytoma patients. The results suggest that there may be certain progression of genetic events that involves chromosomes 1p, 3p, 6p, 11q, 12q, 17q, and 19q in the development of pheochromocytomas, and the activation of ERBB-2 located on chromosome 17q is an important and early event in the malignancy development of these tumor types. The overexpression of ERBB-2 identified by IHC suggested that this oncogene could be associated with the malignancy of pheochromocytomas and paragangliomas

Heat shock transcription factor 1 preserves cardiac angiogenesis and adaptation during pressure overload

To examine how heat shock transcription factor 1 (HSF1) protects against maladaptive hypertrophy during pressure overload, we subjected HSF1 transgenic (TG), knockout (KO) and wild type (WT) mice to a constriction of transverse aorta (TAC), and found that cardiac hypertrophy, functions and angiogenesis were well preserved in TG mice but were decreased in KO mice compared to WT ones at 4 weeks, which was related to HIF-1 and p53 expression. Inhibition of angiogenesis suppressed cardiac adaptation in TG mice while overexpression of angiogenesis factors improved maladaptive hypertrophy in KO mice. In vitro formation of vasculatures by microvascular endothelial cells was higher in TG mice but lower in KO mice than in WT ones. A siRNA of p53 but not a HIF-1 gene significantly reversed maladaptive hypertrophy in KO mice whereas a siRNA of HIF-1 but not a p53 gene induced maladaptive hypertrophy in TG mice. Heart microRNA analysis showed that miR-378 and miR-379 were differently changed among the three mice after TAC, and miR-378 or siRNA of miR-379 could maintain cardiac adaptation in WT mice. These results indicate that HSF1 preserves cardiac adaptation during pressure overload through p53-HIF-1-associated angiogenesis, which is controlled by miR-378 and miR-379

A Mid-infrared Flare in the Active Galaxy MCG-02-04-026: Dust Echo of a Nuclear Transient Event

We report the discovery of a mid-infrared (MIR) flare using Wide field Infrared Survey Explorer data in the center of the nearby Seyfert 1.9 galaxy MCG-02-04-026. The MIR flare began in the first half of 2014, peaked around the end of 2015, and faded in 2017. During these years, energy of more than 7 × 10⁵⁰ erg was released in the infrared, and the flare's MIR color was generally turning red. We detected neither optical nor ultraviolet (UV) variation corresponding to the MIR flare based on available data. We explained the MIR flare using a dust echo model in which the radiative transfer is involved. The MIR flare can be well explained as thermal reradiation from dust heated by UV–optical photons of a primary nuclear transient event. Although the transient event was not seen directly owing to dust obscuration, we can infer that it may produce a total energy of at least ~10⁵¹ erg, most of which was released in less than ~3 yr. The nature of the transient event could be a stellar tidal disruption event by the central supermassive black hole (SMBH), or a sudden enhancement of the existing accretion flow onto the SMBH, or a supernova that was particularly bright

A Mid-infrared Flare in the Active Galaxy MCG-02-04-026: Dust Echo of a Nuclear Transient Event

We report the discovery of a mid-infrared (MIR) flare using WISE data in the center of the nearby Seyfert 1.9 galaxy MCG-02-04-026. The MIR flare began in the first half of 2014, peaked around the end of 2015, and faded in 2017. During these years, energy more than $7\times10^{50}$ erg was released in the infrared, and the flare's MIR color was generally turning red. We detected neither optical nor ultraviolet (UV) variation corresponding to the MIR flare based on available data. We explained the MIR flare using a dust echo model in which the radiative transfer is involved. The MIR flare can be well explained as thermal reradiation from dust heated by UV-optical photons of a primary nuclear transient event. Although the transient event was not seen directly due to dust obscuration, we can infer that it may produce a total energy of at least $\sim10^{51}$ erg, most of which was released in less than $\sim$3 years. The nature of the transient event could be a stellar tidal disruption event by the central supermassive black hole (SMBH), or a sudden enhancement of the existing accretion flow onto the SMBH, or a supernova which was particularly bright.Comment: 44 pages, 13 figures; Accepted to be published in Ap