Integrated Genomic Analysis of the Ubiquitin Pathway across Cancer Types

Protein ubiquitination is a dynamic and reversibleprocess of adding single ubiquitin molecules orvarious ubiquitin chains to target proteins. Here,using multidimensional omic data of 9,125 tumorsamples across 33 cancer types from The CancerGenome Atlas, we perform comprehensive molecu-lar characterization of 929 ubiquitin-related genesand 95 deubiquitinase genes. Among them, we sys-tematically identify top somatic driver candidates,including mutatedFBXW7with cancer-type-specificpatterns and amplifiedMDM2showing a mutuallyexclusive pattern withBRAFmutations. Ubiquitinpathway genes tend to be upregulated in cancermediated by diverse mechanisms. By integratingpan-cancer multiomic data, we identify a group oftumor samples that exhibit worse prognosis. Thesesamples are consistently associated with the upre-gulation of cell-cycle and DNA repair pathways, char-acterized by mutatedTP53,MYC/TERTamplifica-tion, andAPC/PTENdeletion. Our analysishighlights the importance of the ubiquitin pathwayin cancer development and lays a foundation fordeveloping relevant therapeutic strategies

Facile Route for Bio-Phenol Siloxane Synthesis via Heterogeneous Catalytic Method and its Autonomic Antibacterial Property

Eugenol, used as bio-phenol, was designed to replace the hydrogen atom of hydrogenterminated siloxane by hydrosilylation reaction under the presence of alumina-supported platinum catalyst (Pt-Al2O3), silica-supported platinum catalyst (Pt-SiO2) and carbon nanotube-supported platinum catalyst (Pt-CNT), respectively. The catalytic activities of these three platinum catalysts were measured by nuclear magnetic resonance hydrogen spectrometer (1H NMR). The properties of bio-phenol siloxane were characterized by Fourier transform infrared spectrometer (FT–IR), UV-visible spectrophotometer (UV) and thermogravimeter (TGA), and its antibacterial property against Escherichia coli was also studied. The results showed that the catalytic activity of the catalyst Pt-CNT was preferable. When the catalyst concentration was 100 ppm, the reaction temperature was 80 °C and reaction time was 6 h, the reactant conversion rate reached 97%. After modification with bio-phenol, the thermal stability of the obtained bio-phenol siloxane was improved. For bio-phenol siloxane, when the ratio of weight loss reached 98%, the pyrolysis temperature was raised to 663 °C which was 60 °C higher than hydrogenterminated siloxane. Meanwhile, its autonomic antibacterial property against Escherichia coli was improved significantly

Silicone-Based Thermally Conductive Gel Fabrication via Hybridization of Low-Melting-Point Alloy–Hexagonal Boron Nitride–Graphene Oxide

Thermal contact resistance between the microprocessor chip and the heat sink has long been a focus of thermal management research in electronics. Thermally conductive gel, as a thermal interface material for efficient heat transfer between high-power components and heat sinks, can effectively reduce heat accumulation in electronic components. To reduce the interface thermal resistance of thermally conductive gel, hexagonal boron nitride and graphene oxide were hybridized with a low-melting-point alloy in the presence of a surface modifier, humic acid, to obtain a hybrid filler. The results showed that at the nanoscale, the low-melting-point alloy was homogeneously composited and encapsulated in hexagonal boron nitride and graphene oxide, which reduced its melting range. When the temperature reached the melting point of the low-melting-point alloy, the hybrid powder exhibited surface wettability. The thermal conductivity of the thermally conductive gel prepared with the hybrid filler increased to 2.18 W/(m·K), while the corresponding thermal contact resistance could be as low as 0.024 °C/W. Furthermore, the thermal interface material maintained its excellent electric insulation performance, which is necessary for electronic device applications

Improving the Water Resistance and Mechanical Properties of Feather Keratin/Polyvinyl Alcohol/Tris(Hydroxymethyl)Aminomethane Blend Films by Cross-Linking with Transglutaminase, CaCl₂, and Genipin

The high moisture sensitivity of feather keratin/polyvinyl alcohol/tris(hydroxymethyl)aminomethane (FK/PVA/Tris) blend films hinders their application in the packaging field. Thus, in order to improve the water resistance and mechanical properties of such blend films, we attempted cross-linking the blend film with cross-linking agents such as transglutaminase (TG), CaCl2, and genipin. Obvious differences in the morphology of the blended films were observed by scanning electron microscopy before and after cross-linking, indicating that cross-linking can inhibit the phase separation of the blend film. Conformational changes in the blend films after cross-linking were detected by Fourier transform infrared spectroscopy. Importantly, from examination of the total soluble mass, contact angle measurements, and water vapor permeability tests, it was apparent that cross-linking greatly improved the water resistance of the blend films, in addition to enhancing the mechanical properties (i.e., tensile strength and elongation at break). However, cross-linking was also found to reduce the oxygen barrier properties of the blend films. Therefore, cross-linking appears to be an effective method for promoting the application of FK/PVA/Tris blend films in the packaging field

Fabrication of Reactive Poly(Phenyl-Substituted Siloxanes/Silsesquioxanes) with Si‒H and Alkoxy Functional Groups via the Piers–Rubinsztajn Reaction

Poly(phenyl-substituted siloxanes/silsesquioxanes) are obtained by the Piers–Rubinsztajn (PR) reaction of hydrogen-containing siloxanes (HCS) with diphenyldialkoxysilanes such as diphenyldimethoxysilane and diphenyldiethoxysilane catalyzed by tris(pentafluorophenyl)borane. 29Si nuclear magnetic resonance (NMR) spectroscopy, gel permeation chromatography, and refractive index analysis revealed that apart from phenyl substituents and complex structures such as molecular bridges composed of D2Ph2[(C6H5)2Si(OSi)2], structures also existed in these polymers, having high refractive indexes (above 1.50) and high molecular weights (75.60 KDa·mol−1). As revealed by thermogravimetric analysis, these polymers have high thermal stability as well, with temperature at 5% mass loss (T5%) increasing by 182.5 °C and Rw (residual weight ratio) increasing by 5.17 times from 14.63% to 75.60%, as compared to HCS, exhibiting its potential application as resins for resisting strong heat. Such high-refractive-index and temperature-resistant poly(phenyl-substituted siloxanes/silsesquioxanes) with Si–H and alkoxy functional groups can be used as a good addition-type crosslinking agent with adhesion-promoting properties or a special curing agent that can solidify silicone materials through simultaneous addition and condensation reactions, which has potential application in the light-emitting diode (LED) packaging industry

Preparation and Physicochemical Properties of Blend Films of Feather Keratin and Poly(vinyl alcohol) Compatibilized by Tris(hydroxymethyl)aminomethane

Blend films of feather keratin (FK) and synthetic poly(vinyl alcohol) (PVA) that were compatibilized by tris(hydroxymethyl)aminomethane (Tris) were successfully prepared by a solution-casting method. The scanning electron microscopy (SEM) results showed that a phase separation occurred in the FK/PVA/Tris blended system. Analysis by Fourier transform infrared spectroscopy indicated that the main interactions between the three components were hydrogen bonds. In addition, X-ray diffraction analysis showed that the FK/PVA/Tris blend films were partially crystalline. The barrier properties, mechanical properties, and contact angles of the FK/PVA/Tris films were investigated to determine the effects of the PVA and Tris concentrations. More specifically, upon increasing the PVA content, the elongation at break, the hydrophilicity, and the oxygen barrier properties were enhanced. However, at a constant PVA content, an increase in the Tris content caused the oxygen permeability and the contact angle to decrease, while the tensile strength, elongation at break, and oxygen barrier properties were enhanced. These results indicated that the mechanical properties and gas resistance of the FK/PVA/Tris blend films could be successfully improved using the method described herein, confirming that this route provided a convenient and promising means to prepare FK plastics for practical applications

Synthesis and Characterization of Room Temperature Vulcanized Silicone Rubber Using Methoxyl-Capped MQ Silicone Resin as Self-Reinforced Cross-Linker

Methoxyl-capped MQ silicone resin (MMQ) was first synthesized by the hydrosilylation of vinyl-containing MQ silicone resin and trimethoxysilane and then used in condensed room-temperature vulcanized (RTV) silicone rubber as a self-reinforced cross-linker. Results show that modified silicone rubber exhibits good light transmission. Compared with unmodified silicone rubber, the hardness, tensile strength and elongation of MMQ at the break are increased by 26.4 A, 2.68 MPa and 65.1%, respectively. In addition, the characteristic temperature of 10% mass loss is delayed from 353.5 °C to 477.1 °C, the temperature at maximum degradation rate is also delayed from 408.9 °C to 528.4 °C and the residual mass left at 800 °C is increased from 1.2% to 27.7%. These improved properties are assigned to the synergistic effect of the rigid structure of MMQ, the formation of a dense cross-linking structure in polymers and the uniform distribution of MMQ cross-linking agent in RTV silicone rubber

Three-Dimensional Heterostructured Reduced Graphene Oxide-Hexagonal Boron Nitride-Stacking Material for Silicone Thermal Grease with Enhanced Thermally Conductive Properties

The thermally conductive properties of silicone thermal grease enhanced by hexagonal boron nitride (hBN) nanosheets as a filler are relevant to the field of lightweight polymer-based thermal interface materials. However, the enhancements are restricted by the amount of hBN nanosheets added, owing to a dramatic increase in the viscosity of silicone thermal grease. To this end, a rational structural design of the filler is needed to ensure the viable development of the composite material. Using reduced graphene oxide (RGO) as substrate, three-dimensional (3D) heterostructured reduced graphene oxide-hexagonal boron nitride (RGO-hBN)-stacking material was constructed by self-assembly of hBN nanosheets on the surface of RGO with the assistance of binder for silicone thermal grease. Compared with hBN nanosheets, 3D RGO-hBN more effectively improves the thermally conductive properties of silicone thermal grease, which is attributed to the introduction of graphene and its phonon-matching structural characteristics. RGO-hBN/silicone thermal grease with lower viscosity exhibits higher thermal conductivity, lower thermal resistance and better thermal management capability than those of hBN/silicone thermal grease at the same filler content. It is feasible to develop polymer-based thermal interface materials with good thermal transport performance for heat removal of modern electronics utilising graphene-supported hBN as the filler at low loading levels

A Simple Preparation Route for Bio-Phenol MQ Silicone Resin via the Hydrosilylation Method and its Autonomic Antibacterial Property

MQ silicone resins represent a broad range of hydrolytic condensation products of monofunctional silane (M units) and tetrafunctional silane (Q units). In this work, a Bio-Phenol MQ silicone resin (BPMQ) was designed and synthesized by the hydrosilylation of hydrogen containing MQ silicone resin and eugenol in the presence of chloroplatinic acid. The structure, thermal property, and antibacterial property against Escherichia coli of the modified MQ silicone resin were investigated. The results showed that BPMQ has been prepared successfully, and the thermal stability of this modified polymer improved significantly because of the introduction of phenyl in eugenol. The temperature at the maximum degradation rate increased from 250 °C to 422.5 °C, and the residual yields mass left at 600 °C were increased from 2.0% to 28.3%. In addition, its antibacterial property against Escherichia coli was also enhanced markedly without adding any other antimicrobial agents. This improved performance is ascribed to special functional groups in the structure of eugenol. The BPMQ polymer is expected to be applied to pressure-sensitive adhesives and silicone rubber products for the biomedical field due to its reinforcing effect and antioxidant quality