Effects of evodiamine (EVO) on the mRNA expression of Bax and Bcl-2 in H446 and H1688 cells.

<p>Cell lysates were analyzed by RT-PCR. Each experiment was repeated 3 times. Data presented as mean ± standard deviation (n = 3). Untreated H446 or H1688 cells were used as a negative control group. *<i>P</i><0.05 as compared to the control group. ^#<i>P</i><0.05 as compared to corresponding EVO treated group at 24 h. <i>P</i><0.05 as compared to corresponding EVO treated group at 48 h.</p

Effects of evodiamine (EVO) on the protein expression of Cyt C, caspase-12, -8, -9 and -3, Fas and Trail in the H446 and H1688 SCLC cells.

<p>Cell lysates were analyzed by Western blot. Each experiment was repeated 3 times. Data presented as mean ± standard deviation (n = 3). Untreated H446 or H1688 cells were used as a negative control group. *<i>P</i><0.05 as compared to corresponding control group. Fas: factor associated suicide; Trail: tumor necrosis factor-related apoptosis inducing ligand; Cyt C: cytochrome C.</p

Effects of evodiamine (EVO) on the activities of caspase-8 (A), -9 (B) and -3 (C) in H446 cells.

<p>Cell lysates were analyzed by a colorimetric assay of Ac-DEVD-pNA. Each experiment was repeated 3 times. Data presented as mean ± standard deviation (n = 3). Caspase activities were given as arbitrary units (AU) per milligram of protein. Untreated H446 cells were used as a negative control group. *<i>P</i><0.05 as compared to the corresponding control group. ^#<i>P</i><0.05 as compared to corresponding EVO treated group at 24 h. <i>P</i><0.05 as compared to corresponding EVO treated group at 48 h.</p

Evodiamine (EVO) induces apoptosis through two intrinsic caspase-dependent pathways, but not through an extrinsic caspase-dependent pathway.

<p>Evodiamine (EVO) induces apoptosis through two intrinsic caspase-dependent pathways, but not through an extrinsic caspase-dependent pathway.</p

Effects of evodiamine (EVO) on the levels of ROS, Ca²⁺ and ψ_m in H446 and H1688 SCLC cells.

<p>ROS, Ca²⁺ and ψ_m were separately detected by DCF-DA, Fluo-3/AM and JC-1 assays. Each experiment was repeated 3 times. Data presented as mean ± standard deviation (n = 3). Untreated H446 or H1688 cells were used as a negative control group. *<i>P</i><0.05 as compared to corresponding control group.</p