MicroRNA-101 Exerts Tumor-Suppressive Functions in Non-small Cell Lung Cancer through Directly Targeting Enhancer of Zeste Homolog 2

Introduction:Overexpression of the enhancer of zeste homolog 2 (EZH2) protein has been found in broad range of cancer types, including non-small cell lung cancer (NSCLC). Nevertheless, the mechanisms by which EZH2 becomes overexpressed in NSCLC remain unclear. MicroRNAs (miRNAs) can regulate target gene expression through translational control. In this study, we investigate whether miRNA (miR-101) regulates EZH2 expression in NSCLC.Methods:We evaluated the expression of miR-101 and EZH2 in 20 matched NSCLC and adjacent nontumor lung tissues by reverse-transcriptase polymerase chain reaction and immunohistochemistry, respectively. Luciferase reporter assay was used to determine whether miR-101 directly targets EZH2. To assess the effect of miR-101 on NSCLC biological behavior, cell proliferation, invasion, and response to chemotherapy were analyzed using NSCLC cells transfected with miR-101 mimics or transfected with specific small interfering RNA to deplete EZH2 (small interfering RNA-EZH2).Results:Reduced expression of miR-101 was associated with overexpression of EZH2 in NSCLC tumor tissues. Transfection of miR-101 mimics significantly suppressed the activity of the luciferase reporter containing wild type but not mutant EZH2 3′-UTR and decreased EZH2 expression in NSCLC cell lines. Furthermore, enforced expression of miR-101 or knockdown of EZH2 led to reduced NSCLC cell proliferation and invasion and sensitized cancer cells to paclitaxel-mediated apoptosis through inducing expression of the proapoptotic protein Bim.Conclusions:miR-101 inhibits cell proliferation and invasion and enhances paclitaxel-induced apoptosis in NSCLC cells, at least in part, by directly repressing EZH2 expression. Therapeutic strategies to rescue miR-101 expression or silence EZH2 may be beneficial to patients with NSCLC in the future

Phase Compensation Enhancement of Photon Pair Entanglement Generated from Biexciton Decays in Quantum Dots

Exciton fine-structure splittings within quantum dots introduce phase differences between the two biexciton decay paths that greatly reduce the entanglement of photon pairs generated via biexciton recombination. We analyze this problem in the frequency domain and propose a practicable method to compensate the phase difference by inserting a spatial light modulator, which substantially improves the entanglement of the photon pairs without any loss.Comment: 4 pages, 3 figure

Comparison of Chebyshev and Legendre Polynomial Expansion of Phase Function of Cloud and Aerosol Particles

Chebyshev and Legendre polynomial expansion is used to reconstruct the Henyey-Greenstein phase function and the phase functions of spherical and nonspherical particles. The result of Legendre polynomial expansion is better than that of Chebyshev polynomial for around 0-degree forward angle, while Chebyshev polynomial expansion produces more accurate results in most regions of the phase function. For large particles like ice crystals, the relative errors of Chebyshev polynomial can be two orders of magnitude less than those of Legendre polynomial

An auxiliary ordinary differential equation and the exp-function method

AbstractIn this paper, the new idea of finding the exact solutions of the nonlinear evolution equations is introduced. The idea is that the exact solutions of the auxiliary ordinary differential equation are derived by using exp-function method, and then the exact solutions of the nonlinear evolution equations are derived with aid of the auxiliary ordinary differential equation. As examples, the classical KdV equation, Boussinesq equation, (3+1)-dimensional Jimbo–Miwa equation and Benjamin–Bona–Mahony equation are discussed and the exact solutions are derived