The sequences and secondary structures of five novel rat pre-miRNAs homologous to known miRNAs in other species.

<p>2a. rno-mir-1839. 2b. rno-mir-509. 2c. rno-mir-3068. 2d. rno-mir-1306. 2e. rno-mir-1843. The sequences of 5 novel rat homologous pre-miRNAs hairpin are depicted above their dot-bracket notation secondary structures as determined by RNAfold <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034394#pone.0034394-Gruber1" target="_blank">[62]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034394#pone.0034394-Hofacker1" target="_blank">[63]</a> using minimum free energy algorithm (MFE). RNAfold is a widely used webserver to predict RNA secondary structure. Below the dot-bracket notation secondary structures of these novel rat homologous pre-miRNAs, each of the small RNA sequences that matched those pre-miRNAs hairpin are listed, with the number of reads representing each sequence at its right side. The mature and the mature* sequences are marked in red and green respectively. The MFEs of those rat novel pre-miRNAs predicted by RNAfold are above their sequences. The single nucleotide extension isomirs of the mature* sequences had higher read counts than the mature* sequences with perfect 2 nt 3′ overhang in two miRNAs (rno-miR-3068-3p and rno-miR-1843-3p).</p

Four rat-specific novel miRNAs - Mature sequences and genome locations.

<p>Four rat-specific novel miRNAs - Mature sequences and genome locations.</p

Improved Ethanol Adsorption Capacity and Coefficient of Performance for Adsorption Chillers of Cu-BTC@GO Composite Prepared by Rapid Room Temperature Synthesis

A composite of Cu-BTC and graphite oxide (GO) was prepared by rapid room-temperature synthesis method for thermally driven adsorption chillers (TDCs). A series of composites Cu-BTC@GO with varied GO loading were synthesized at room temperature within 1 min, and characterized by N₂ adsorption test, scanning electron microscopy, powder X-ray diffraction, and Fourier transform infrared analysis. The adsorption isotherms of ethanol on the composites were measured at different temperatures, and then, the isosteric heats of ethanol adsorption were estimated. The composite working capacities and coefficient of performance (COP) of the composite–ethanol working pair were calculated for the application of refrigeration. Results showed that Cu-BTC@GO possessed a superhigh adsorption capacity for ethanol up to 13.60 mmol/g at 303 K, which was attributed to introduction of GO leading to increases in the surface dispersive forces and the mesoporous volume of Cu-BTC@GO. The isosteric heat of ethanol adsorption on Cu-BTC@GO was slightly higher than that of Cu-BTC. The adsorption capacity of Cu-BTC@GO was higher than many other metal–organic frameworks (MOFs) under the application conditions of TDCs. The composites exhibited 5.8–17.4% higher working capacity and energy efficiency than parent Cu-BTC for the application of refrigeration. The rapid room-temperature synthesis approach has potential for the preparation of new MOF-based composites

Four rat-specific novel pre-miRNAs sequences.

<p>Four rat-specific novel pre-miRNAs sequences.</p

The sequences and secondary structures of the four novel rat specific pre-miRNAs.

<p>3a. rno-mir-3598. 3b. rno-mir-3599. 3c. rno-mir-3600. 3d. rno-mir-3601. The sequences of 4 novel rat specific pre-miRNAs are depicted above their dot-bracket notation secondary structures as determined by RNAfold <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034394#pone.0034394-Gruber1" target="_blank">[62]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034394#pone.0034394-Hofacker1" target="_blank">[63]</a> using MFE. RNAfold is a widely used webserver to predict RNA secondary structure. Below the dot-bracket notation secondary structures of these rat specific pre-miRNA, each of the small RNA sequences that matched those pre-miRNAs hairpin are listed, with the number of reads representing each sequence at its right side. The mature and the mature* sequences are marked in red and green, respectively. The MFEs of those rat specific miRNAs predicted by RNAfold are above their pre-miRNA sequences. For rno-miR-3598, the inferred mature* sequence is shown in green in the secondary structure.</p

Homologous miRNAs and homologous sequences related to ten novel rat homologous miRNAs.

<p>Homologous miRNAs and homologous sequences related to ten novel rat homologous miRNAs.</p

Five novel rat homologous pre-miRNAs sequences.

<p>Five novel rat homologous pre-miRNAs sequences.</p

Ten rat homologous miRNAs sequences and genome locations.

<p>Note: The names of novel rat mature and mature* miRNAs are marked in bold and regular font, respectively. Sequences of three novel rat miRNAs differ from homologous sequences related to other species and those different bases are marked in italic font. All miRNAs show a perfect 2 nt 3′ overhang, except rno-miR-3068-3p and rno-miR-1843-3p marked with (#). Those two miRNAs have perfect Dicer pattern with the second most expressed mature* read, while the most expressed mature* read probably is a single nucleotide extension isomiR.</p

Anchoring Sb₆O₁₃ Nanocrystals on Graphene Sheets for Enhanced Lithium Storage

Sb₆O₁₃/reduced graphene oxide (Sb₆O₁₃/rGO) nanocomposite was synthesized by the solvothermal method using Sb₂O₃ and graphene oxide as raw material. On the basis of the physical and electrochemical characterizations, Sb₆O₁₃ nanocrystals of 10–20 nm size were uniformly anchored on rGO sheets, and the nanocomposite displayed a large reversible specific capacity of 1271 mA h g^–1 and an excellent cyclability of 1090 mA h g^–1 after 140 cycles at 100 mA g^–1 when proposed as a potential anode material for lithium ion batteries, emphasizing the advantages of anchoring of Sb₆O₁₃ nanocrystals on rGO sheets for the maximum utilization of electrochemically active Sb₆O₁₃ and rGO for lithium storage

A Scalable Epitope Tagging Approach for High Throughput ChIP-Seq Analysis

Eukaryotic transcriptional factors (TFs) typically recognize short genomic sequences alone or together with other proteins to modulate gene expression. Mapping of TF-DNA interactions in the genome is crucial for understanding the gene regulatory programs in cells. While chromatin immunoprecipitation followed by sequencing (ChIP-Seq) is commonly used for this purpose, its application is severely limited by the availability of suitable antibodies for TFs. To overcome this limitation, we developed an efficient and scalable strategy named cmChIP-Seq that combines the clustered regularly interspaced short palindromic repeats (CRISPR) technology with microhomology mediated end joining (MMEJ) to genetically engineer a TF with an epitope tag. We demonstrated the utility of this tool by applying it to four TFs in a human colorectal cancer cell line. The highly scalable procedure makes this strategy ideal for ChIP-Seq analysis of TFs in diverse species and cell types