28 research outputs found

    Accident risks of LNG-fueled ships during navigation and lockage processes.

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    <p>Accident risks of LNG-fueled ships during navigation and lockage processes.</p

    Consequences of flash-fire accidents under different failure modes.

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    <p>Consequences of flash-fire accidents under different failure modes.</p

    Study on lockage safety of LNG-fueled ships based on FSA

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    <div><p>In the present study, formal safety assessment (FSA) is introduced to investigate lockage safety of LNG-fueled ships. Risk sources during lockage of LNG-fueled ships in four typical scenarios, namely, navigation between two dams, lockage, anchorage, and fueling, are identified, and studied in combination with fundamental leakage probabilities of various components of LNG storage tanks, and simulation results of accident consequences. Some suggestions for lockage safety management of LNG-fueled ships are then proposed. The present research results have certain practical significance for promoting applications of LNG-fueled ships along Chuanjiang River and in Three Gorges Reservoir Region.</p></div

    Flow chart of FSA method.

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    <p>Flow chart of FSA method.</p

    Flash fire/VCE risk under different failure modes.

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    <p>Flash fire/VCE risk under different failure modes.</p

    Consequences of VCE accidents under different failure modes.

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    <p>Consequences of VCE accidents under different failure modes.</p

    LNG accident probabilities under different failure modes.

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    <p>LNG accident probabilities under different failure modes.</p

    Individual risk standards in China.

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    <p>Individual risk standards in China.</p

    Structural insights into stereospecific reduction of α, β-unsaturated carbonyl substrates by old yellow enzyme from <i>Gluconobacter oxydans</i>

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    <div><p>We report the crystal structure of old yellow enzyme (OYE) family protein Gox0502 (a.a 1–315) in free form at 3.3 Å. Detailed structural analysis revealed the key residues involved in stereospecific determination of Gox0502, such as Trp66 and Trp100. Structure-based computational analysis suggested the bulky side chains of these tryptophan residues may play important roles in product stereoselectivity. The introduction of Ile or Phe or Tyr mutation significantly reduced the product diastereoselectivity. We hypothesized that less bulky side chains at these critical residues could create additional free space to accommodate intermediates with different conformations. Notably, the introduction of Phe mutation at residue Trp100 increased catalytic activity compared to wild-type Gox0502 toward a set of substrates tested, which suggests that a less bulky Phe side chain at residue W100F may facilitate product release. Therefore, Gox0502 structure could provide useful information to generate desirable OYEs suitable for biotechnological applications in industry.</p></div
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