Longitudinal view of the organisation of elastin fibres in the tibial fibrous and hyaline cartilage.

<p>In the fibrous cartilage, horizontal elastin fibres were found in the superficial zone (A); longitudinal elastin fibres with branches were found in the middle (B) and deep (C) zones. In the hyaline cartilage, elastin fibres were parallel to the articular surface and only found in the superficial zone (D). There were no resolvable elastin fibres in the ECM but fine elastin around chondrocytes was found in the middle (E) and deep (F) zones. Scale bar = 20 µm.</p

Typical histomorphology of the kangaroo tibial fibrous cartilage and hyaline cartilage.

<p>Loose collagen bundles with few chondrocytes were found parallel to the articular surface in the superficial zone (B). Oblique collagen fibres (arrows) were found in the middle zone (C). Long oblique collagen fibres were found in the deep zone (D). No obvious collagen orientation was found in the calcified zone (E). A relatively higher chondrocyte density was found in zones of hyaline cartilage (b–e) but the orientation of collagen fibres was not obvious except in the deep zone where longitudinal collagen fibres were found (d).</p

The anatomy of the articular surface of kangaroo tibial plateau, and the creep behaviour of fibrous cartilage and hyaline cartilage.

<p>Menisci cover approximately 70% of the medial tibial plateau and 80% the lateral tibial plateau (A). The fibrous cartilage is located in the central area and accounts for approximate 55% of the medial and 50% of the lateral tibial plateau surfaces (B). The middle area of tibial plateau was marked divided into three parts (#1, 2 and 3) and the region #2 was subjected to indentation tests. Higher compressive strain in the fibrous cartilage shown from 10 seconds to 60 seconds of the test indicated that the fibrous cartilage was more readily deformed than hyaline cartilage (**<i>P</i><0.0001) (C). N.S = not significant.</p

PG distribution of the kangaroo tibial fibrous cartilage and hyaline cartilage indicated by safranin O – fast green staining.

<p>In both fibrous cartilage and hyaline cartilage, PG concentration increased from little in superficial zone to a maximum in the deep zone and then decreased in the calcified zone. However, compared to the hyaline cartilage, the fibrous cartilage showed higher PG concentrations in all zones except in the superficial zone.</p

Transverse view of the organisation of elastin fibres in the tibial fibrous and hyaline cartilage.

<p>Horizontal elastin fibres were found throughout the tissue depth of the fibrous cartilage, including the superficial (A), middle (B) and deep (C) zones. In the hyaline cartilage, elastin fibres were parallel to the articular surface and only found in the superficial zone (D). There were no resolvable elastin fibres in the ECM but fine elastin around chondrocytes was found in the middle (E) and deep (F) zones. Scale bar = 20 µm.</p

Longitudinal view of the organization of collagen in kangaroo tibial fibrous and hyaline cartilage.

<p>In the fibrous cartilage, horizontal collagen was found in the superficial zone (A); a mix of oblique collagen in the longitudinal direction was found in the middle zone (arrows, B); and both horizontal and longitudinal collagen was found in the deep zone (arrows, C). In the hyaline cartilage, horizontal collagen was found in the superficial zone (D); a mix of in different directions was found in the middle zone (B); and longitudinal collagen was found in the deep zone (F). Scale bar = 20 µm.</p

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