Additional file 3: Figure S1. of Histone modification signature at myeloperoxidase and proteinase 3 in patients with anti-neutrophil cytoplasmic autoantibody-associated vasculitis

Comparison of therapy in patients during remission on expression of histone modifying genes, EHMT1, EHMT2, ING4, and MSL1. (PDF 93.6 kb

Additional file 4: Table S3. of Histone modification signature at myeloperoxidase and proteinase 3 in patients with anti-neutrophil cytoplasmic autoantibody-associated vasculitis

Characteristics of patients with ANCA disease used for chromatin immunoprecipitation. (PDF 46.2 kb

Pellionia minima Makino

原著和名: サンセウサウ科名: イラクサ科 = Urticaceae採集地: 愛知県 鳳来寺山 (三河 鳳来寺山)採集日: 1961/5/21採集者: 萩庭丈壽整理番号: JH007848国立科学博物館整理番号: TNS-VS-95784

Characteristics of patients with ANCA disease enrolled in the Affymetrix microarray study.

<p>Characteristics of patients with ANCA disease enrolled in the Affymetrix microarray study.</p

Frequency of PTPN22 of protective allele (<i>A788</i>) in Caucasian ANCA patients.

<p>Frequency of PTPN22 of protective allele (<i>A788</i>) in Caucasian ANCA patients.</p

Meta-analysis of the frequency of the <i>PTPN22 C1858T</i> SNP in ANCA Disease.

<p>Meta-analysis of the frequency of the <i>PTPN22 C1858T</i> SNP in ANCA Disease.</p

Characteristics of patients with ANCA disease enrolled in the functional studies.

*<p>Patient's sample included in western blot analysis of signaling pathways.</p

<i>IL-10</i> mRNA expression.

<p>(A) <i>IL-10</i> transcript levels are reduced in leukocytes from PTPN22 (R620W) positive patients. (A) IL-10 mRNA expression, which is mediated through the ERK pathway, was significantly lower in gain-of-function patients (<i>p</i><0.0001) by quantitative TaqMan PCR. (B) Longitudinally, the baseline level of IL-10 message in patients with the gain-of-function variant did not increase as they transitioned from active disease to remission (<i>p</i> = 0.25). (C) In contrast, patients with normal PTPN22 showed a robust increase in IL-10 as they entered remission (<i>p</i><0.0001). (D) Decreased IL-10 levels were associated with the relapsing group (n = 39, 1.8±1.15), and higher level in the non-relapse patient group (n = 14, 6.6±4.4, <i>p</i><0.0001).</p

Bioinformatics analysis of Affymetrix microarray gene expression data, comparing leukocytes with the gain-of-function genotype to those with a non-variant genotype.

<p>Principal Component Analysis (PCA) scatter plot using Partek analysis is shown in the upper left corner. PCA is mathematically defined as an orthogonal linear transformation that transforms the data to a new coordinate system such that the greatest variance by any projection of the data comes to lie on the first coordinate (called the first principal component), the second greatest variance on the second coordinate, and so on. Each dot represents a patient's expression profile; the blue color dots represent gain-of-function and red show non-variant genotypes. Analysis using the Ingenuity Pathway Tools (IPA) software utilizes a repository of biological interactions and functional annotations created from millions of individually modeled relationships. The genes in red indicate increased expression and blue represents decreased expression, comparing gain-of-function with non-variant individuals. Primary networks identified were ERK1/2, p38MAPK, and NFκB networks.</p

Frequency of PTPN22 risk-allele (<i>T1858</i>) genotype in Caucasian ANCA patients.

<p># Zheng W, 2005 (35).</p>*<p>Excluded from analysis: 6 ANCA-neg; 5 PR3+MPO dual serology: 3 p-ANCA+ANA positives.</p