Spherical Indicatrices of a Bertrand Curve in Three Lie Groups

In this paper, new representations of a Bertrand curve pair in three dimensional Lie groups with bi-invariant metric are given. Besides, the spherical indicatrices of a Bertrand curve pair are obtain and the relations between the spherical indicatrices and new representations of Bertrand curve pair are shown.Comment: 12 page

Discrimination and Geographical Origin Prediction of Cynomorium songaricum Rupr. from Different Growing Areas in China by an Electronic Tongue

Cynomorium songaricum Rupr. is a well-known and widespread plant in China. It has very high medicinal values in many aspects. The study aimed at discriminating and predicting C. songaricum from major growing areas in China. An electronic tongue was used to analyze C. songaricum based on flavor. Discrimination was achieved by principal component analysis and linear discriminant analysis. Moreover, a prediction model was established, and C. songaricum was classified by geographical origins with 100% degree of accuracy. Therefore, the identification method presented will be helpful for further study of C. songaricum

Visualization 1.mp4

Tomographic reconstruction of two enlarged regions of human buccal epithelial cell

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3D RI tomograms rendering of HeLa cell

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3D RI tomograms rendering of Pandorina morum algae

miR-17-3p Downregulates Mitochondrial Antioxidant Enzymes and Enhances the Radiosensitivity of Prostate Cancer Cells

Radioresistance remains to be a major obstacle in the management of patients with advanced prostate cancer (PCa). We have identified a mature miR-17-3p processed from the 3′ arm of precursor miR-17, which appeared to be able to inhibit three major antioxidant enzymes located in mitochondria, i.e., manganese superoxide dismutase (MnSOD), glutathione peroxidase 2 (Gpx2), and thioredoxin reductase 2 (TrxR2). Here we show that upregulation of miR-17-3p remarkably sensitized PCa cells to ionizing radiation (IR). Reductions of the three antioxidants led to increasing cellular reactive oxygen species (ROS) accumulation as well as declining mitochondrial respiration. The miR-17-3p-mediated dysfunction of mitochondrial antioxidants apparently sensitizing IR therapy was manifested in vitro and in vivo. Substantially, the miR-17-3p effect on suppression of the antioxidants can be efficiently eliminated or attenuated by transfecting with either an miR-17-3p inhibitor or each of the related antioxidant cDNA expression constructs. Overall, in addition to the insights into the functional assessments for the duplex of miR-17-5p and miR-17-3p, the present study highlights the rigorous evidence that demonstrated suppression of multiple mitochondrial antioxidants by a single microRNA (miRNA), thereby providing a promising approach to improve radiotherapy for advanced PCa by targeting mitochondrial function. Keywords: miR-17-3p, antioxidant enzymes, reactive oxygen species, radiosensitivity, prostate cance

HZ08 suppresses RelB-activated MnSOD expression and enhances Radiosensitivity of prostate Cancer cells

Abstract Background The development of radioresistance is one of main causes for therapeutic failure of prostate cancer (PCa). The present study aims to investigate the function and the related mechanism by which HZ08 sensitizes radiotherapeutic efficiency to treat aggressive PCa cells. Methods PCa cells were pretreated with HZ08 (6,7-dimethoxy-1-(3,4-dimethoxy) benzyl-2-(N-n-octyl-N′-cyano) guanyl-1,2,3,4-tetrahydroisoquinoline) and followed by ionizing radiation (IR) treatment. Cytotoxicity in the treated cells was analyzed to assess the radiosensitization capacity of HZ08 by flow cytometry, MTT and colony survival assays. The cellular levels of reactive oxygen species (ROS) and oxygen consumption rates (OCR) were measured using specific ROS detection probes and a Seahorse XF96 Analyzer, respectively. RelB binding to the NF-κB intronic enhancer region of the human SOD2 gene was determined using a ChIP assay. The levels of phosphorylation of PI3K, Akt and IKKα were quantified and further confirmed using a PI3K inhibitor. Finally, the synergistic effect of HZ08 on radiosensitization of PCa cells was validated using a mouse xenograft tumor model. Results HZ08 enhanced radiosensitivity of PCa cells through increasing ROS and declining mitochondrial respiration due to suppression of mitochondrial antioxidant enzyme MnSOD. Mechanistically, HZ08 appeared to inhibit PI3K/Akt/IKKα signaling axis, resulting in transcriptional repression of MnSOD expression by preventing RelB nuclear translocation. Conclusions HZ08 can serve as a useful radiosensitizing agent to improve radiotherapy for treating aggressive PCa cells with high level of constitutive RelB. The present study suggests a promising approach for enhancing radiotherapeutic efficiency to treat advanced PCa by inhibiting antioxidant defense function

Additional file 3 of HZ08 suppresses RelB-activated MnSOD expression and enhances Radiosensitivity of prostate Cancer cells

: Figure S3. The reverse effect of transfected MnSOD on cell viability of HZ08 and IR-treated cells. PC-3 (A) and DU-145 cells (B) were transfected with a MnSOD expression construct, and then treated with 5 μM HZ08 and 6 Gy IR. Cell viability was quantified by MTT. Mean ± SD was representative of three independent experiments carried out in duplication. **(P < 0.01) shows the significances between two groups as indicated. (PDF 390 kb