Gene ontology (GO) function classification of differentially expressed genes (DEGs) in <i>B</i>. <i>napu</i>s.

<p>A total of 1,763 genes were assigned to the three main GO functional categories and then divided into 57 sub-categories.</p

Statistics of the seed weight, seed size and fresh seed weight at different periods of development.

<p>Statistics of the seed weight, seed size and fresh seed weight at different periods of development.</p

Hierarchical clustering analysis of DEGs based on FPKM data between T3 and T4.

<p>The colour key represents the FPKM (Fragments Per Kilobase of exon per Million fragments mapped) normalized log₂ transformed counts. Red represents high expression, and blue represents low expression. Each row represents a gene.</p

KEGG pathway analysis identified the ubiquitin-mediated proteolysis pathway (ko04120) and proteasome pathway (ko03050) for 15 DEGs between T3 and T4.

<p>(A) ko04120, and (B) ko03050.</p

Clusters of orthologous groups (COG) function classification of differentially expressed genes (DEGs) in <i>B</i>. <i>napu</i>s.

<p>A total of 678 DEGs were distributed across 25 COG categories.</p

Summary of key enzymes and proteins related to seed weight in the Swiss-Prot and NR databases.

<p>Summary of key enzymes and proteins related to seed weight in the Swiss-Prot and NR databases.</p

Statistics of RNA-seq data aligned to the reference genome.

<p>Statistics of RNA-seq data aligned to the reference genome.</p

Functional annotation of new genes and differentially expressed genes in the COG, GO, KEGG, Swiss-Prot and NR databases.

<p>Functional annotation of new genes and differentially expressed genes in the COG, GO, KEGG, Swiss-Prot and NR databases.</p

Comparison of the raw transcriptome data for the large-seed line and small-seed line in <i>B</i>. <i>Napus</i>.

<p>Comparison of the raw transcriptome data for the large-seed line and small-seed line in <i>B</i>. <i>Napus</i>.</p

Summary of DEGs related to seed weight (ubiquitin-mediated proteolysis pathway and proteasome pathway) in the KEGG database between T3 and T4.

<p>Summary of DEGs related to seed weight (ubiquitin-mediated proteolysis pathway and proteasome pathway) in the KEGG database between T3 and T4.</p