Ercc6l identities between Sika deer and 12 other organisms.

<p>Ercc6l identities between Sika deer and 12 other organisms.</p

Principal component analysis of the composition of functional groups obtained from 1999 and 2009, separately.

<p>Vertical and horizontal bars represent the standard deviations (<i>n</i> = 3). Each point represents a specific SOC chemical structure in fertilization or CK treatments. Points that are close together are more similar to one another than points that are far apart.</p

Soil organic carbon (SOC) content as affected by fertilization treatment and time.

<p>Treatments followed by different letters are significantly different at <i>p</i> < 0.05 (Tukey test, n = 3).</p

ClustalX alignment of the Ercc6l protein sequence with other members of SNF2 family as reported in other mammals.

<p>The conserved amino acid residues in these sequences are shaded to show homology.</p

Organs distribution of fetal Sika deer (labeled FSD) and adult Sika deer Ercc6l (labeled ASD).

<p>mRNAs in the Sika deer were detected by RT-PCR. (a) After 25 cycles, FSD was detected in the heart (H), liver (L), spleen (S), lung (LU), kidney (K), stomach (ST), brain (B), intestines (I), ear (E), lymph (LY), leg (LE) and negative (N); (b) FSD and ASD were detected in organs such as heart (H), liver (L), spleen (S), lung (LU), kidney (K), stomach (ST) and negative (N).</p

A phylogenic tree showing the similarity between Sika deer Ercc6l protein amino acid sequences and those of cattle, panda, dog, horse, fowl, human, monkey, mouse, pongo, rat, xenopus and marmoset.

<p>The number at each node indicates the percentage of bootstrapping after 1000 replications. The bar at the bottom indicates 5% amino acid divergence in the sequences. These proteins are highly conserved throughout evolution; high similarity matches exist between Ercc6l sequences across species.</p

The cDNA sequences of Sika deer were obtained from heart mRNA using 5′ RACE and 3′ RACE.

<p>(A) Products obtained during the first 3′ RACE were 446 bp in length. (B) Products obtained during the second 3′ RACE were 445 bp in length. (C) Products obtained during a third 3′ RACE were 546 bp in length. (D) 5′ RACE products were 444 bp in length.</p

Organs distribution of the Sika deer Ercc6l transcript as measured by SYBR Green RT-PCR.

<p>Quantitative analysis was performed on Ercc6l gene expression relative to β-actin in different Organs and different developmental stages. Ercc6l gene expression was analyzed in the following Organs: heart, liver, spleen, lung, kidney and stomach. Expression data for each Organ were analyzed from three individual Sika deer. Vertical bars represent the mean±S.E.</p

Composition of C functional groups (%) estimated from relative integration values of ¹³C CPMAS NMR spectra of soil samples.

<p>Treatments followed by different letters in a column are significantly different at <i>p</i> < 0.05 (Tukey test, n = 3).</p><p>Composition of C functional groups (%) estimated from relative integration values of ¹³C CPMAS NMR spectra of soil samples.</p

Solid-state CPMAS ¹³C NMR spectra of the soils collected in 1989, 1999 and 2009, separately.

<p>89 = soils collected in 1989, 99 = soils collected in 1999, 09 = soils collected in 2009.</p