Ferrocenyl-Appended Aurone and Flavone: Which Possesses Higher Inhibitory Effects on DNA Oxidation and Radicals?

The aim of the present work was to compare the antioxidative effect of the ferrocenyl-appended aurone with that of ferrocenyl-appended flavone; therefore, nine aurones together with the flavone-type analogues were synthesized by using chalcone as the reactant. The radical-scavenging property was evaluated by reacting with the 2,2′-azinobis­(3-ethylbenzothiazoline-6-sulfonate) cationic radical (ABTS^<b>+·</b>), 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH), and galvinoxyl radical, respectively. The cytotoxicity was estimated by inhibiting 2,2′-azobis­(2-amidinopropane hydrochloride) (AAPH)-induced oxidation of DNA. It was found that the introduction of the ferrocenyl group remarkably increased the radical-scavenging activities of aurone and flavone. Especially, the ferrocenyl group in flavones can quench radicals even in the absence of the phenolic hydroxyl group, while ferrocenyl-appended aurones can efficiently protect DNA against AAPH-induced oxidation. Therefore, the antioxidative effect was generated by the ferrocenyl group and enhanced by the electron-donating group attaching to the <i>para</i>-position of the ferrocenyl group. Introducing the ferrocenyl group into natural compounds may be a useful strategy for increasing the antioxidative effectiveness

Stress and anxiety in couples submitted to assisted reproduction.

Este trabalhou avaliou o stress e a ansiedade em 30 casais, que se submeteram à reprodução assistida no momento anterior à coleta dos óvulos e/ou espermatozóides, tendo um tempo de infertilidade que variou de 1 a 17 anos. Para avaliação da ansiedade foi aplicado o STAI-STATE TRAIT ANXIETY INVENTORY (STAI) e o Stress foi avaliado através do SCOPE-STRESS. No STAI foi acrescentada uma escala visual analógica para medir também a intensidade da ansiedade. A Ficha de Identificação avaliou questões como: idade, sexo, nacionalidade, profissão, ocupação, religião, grau de instrução, renda mensal, estado civil, tempo de casado e questões como: tempo de infertilidade, filhos naturais ou adotivos, profissionais implicados no tratamento, a existência de tentativas anteriores e os momentos de maior tensão emocional nas tentativas anteriores e a atual. Dentre os 36 sujeitos que já haviam feito tentativas anteriores de Reprodução Assistida, um dos três momentos de maior tensão emocional foi o de aguardar a gravidez. Dentre os 60 sujeitos, ou seja, todos os que estão na tentativa atual, aguardar a gravidez foi também um dos três momentos que geraram maior tensão. O diagnóstico de infertilidade foi misto em 33,3% dos casais, apenas feminino em 20% e apenas masculino em 46,7% dos casais. As mulheres apresentaram grau de ansiedade significantemente maior que os homens quanto às escalas Stai-Trait freqüência e intensidade e semelhantes quanto às escalas Stai-State freqüência e intensidade. Não houve diferença significante entre os escores médios dos homens e mulheres quanto às medidas descritivas do Scope-Stress.This work evaluated stress and anxiety in 30 couples submitted to assisted reproduction, with an infertility period that ranged from 1 to 17 years, the moment just before the oocyte retrieval and/or semen sample. For anxiety evaluation the Stai-State Trait Anxiety Inventory (STAI) was applied, and stress was evaluated using the Scope-Stress. While applying STAI, a visual analogic scale was added to measure the intensity of anxiety. The identification form included information such as: age, gender, nationality, profession, occupation, religion, school level, monthly income, marital status, married time and issues such as: infertility period, existence of natural or adoptive children, professionals involved in infertility treatment, existence of previous attempts and the moments of major emotional stress during the previous attempts and during the present one. Among the 36 subjects submitted to previous attempts of Assisted Reproduction, one of the three moments of major emotional stress was the attendance of pregnancy confirmation. Among the all 60 subjects submitted to the present attempt, attendance of pregnancy confirmation also was one of the three moments of major emotional stress. Infertility diagnosis was mixed in 33,3% of the couples, exclusively feminine in 20% and exclusively masculine in 46,7% of the couples. Women presented a significantly higher anxiety degree than men, regarding the STAI-TRAIT scales of frequency and intensity and similar regarding the STAI-STATE scales of frequency and intensity. There was no significant difference between the mean scores of men and women regarding descriptive measures of the SCOPE-STRESS

Slowing the Starch Digestion by Structural Modification through Preparing Zein/Pectin Particle Stabilized Water-in-Water Emulsion

Slowing the digestion of starch is one of the dominant concerns in the food industry. A colloidal structural modification strategy for solving this problem was proposed in this work. Due to thermodynamic incompatibility between two biopolymers, water/water emulsion of waxy corn starch (WCS) droplets dispersed in a continuous aqueous guar gum (GG) was prepared, and zein particles (ZPs), obtained by antisolvent precipitation and pectin modification, were used as stabilizer. As the ratio of zein to pectin in the particles was 1:1, their wetting properties in the two polysaccharides were similar, which made them accumulate at the interface and cover the WCS-rich droplets. The analysis of digestibility curves indicated that a rapid (rate constant <i>k</i>₁: 0.145 min^–1) and a slow phase (<i>k</i>₂: 0.022 min^–1) existed during WCS digestion. However, only one slow phase (<i>k</i>₂: 0.019 min^–1) was found in the WCS/GG emulsion, suggesting that this structure was effective in slowing starch digestion

Development and Validation of an Osteoporosis Self-Assessment Tool for Taiwan (OSTAi) Postmenopausal Women-A Sub-Study of the Taiwan OsteoPorosis Survey (TOPS)

<div><p>Background</p><p>To develop an OSTAi tool and compare this with the National Osteoporosis Foundation recommendations in 2013 (NOF 2013) for bone mineral density (BMD) testing among Taiwan postmenopausal women.</p><p>Methods</p><p>Taiwan Osteoporosis Association (TOA) conducted a nationwide BMD survey by a bus installed with a dual energy X-ray absorptiometry (DXA) between 2008 and 2011. All of the participants completed questionnaire, which included demographics and risk factors of osteoporotic fracture in FRAX tool. We used the database to analyze potential risk factors for osteoporosis and followed the model by Koh et al. to develop a risk index via multiple variable regression analysis and item reduction. We used the index values to set up a simple algorithm (namely OSTAi) to identify those who need BMD measurement. Receiver operating characteristic (ROC) curve and the area under the curve (AUC) was used to compare the sensitivity/specificity analysis of this model with that of recommendations by NOF 2013.</p><p>Results</p><p>A total of 12,175 Taiwan postmenopausal women enrolled in this survey. The index value was derived by age and body weight of the participants according to weighted odds of each risk factor and the selected cutoff value was set at “-1”. There are 6393 (52.5%) participants whose index value is below “-1” and whose risk of osteoporosis was 57.5% (3674/6393). The AUC for OSTAi and NOF 2013 were 0.739 (95% confidence interval (CI), 0.728–0.749, P<0.001) and 0.618 (95% CI, 0.606–0.630, P<0.001), respectively. The sensitivity and specificity of OSTAi, at the selected cutoff value of -1, and NOF 2013 to identify osteoporosis were 73.1%, 62.0% and 78.3%, 45.7%, respectively.</p><p>Conclusions</p><p>As OSTA for Asian populations, OSTAi is an useful tool to identify Taiwan postmenopausal women with osteoporosis, In comparison with NOF 2013, OSTAi may be an easier and better tool for referral to BMD measurement by DXA in this area.</p></div

Number of woman with T-score at -2.5 or below according to site.

<p>Number of woman with T-score at -2.5 or below according to site.</p

Demographics of participants.

<p># Lowest T-score among lumbar spine, femoral neck and total hip</p><p>* Definition same as those of FRAX</p><p>@ n = answered yes in the questionnaire,</p><p>N = total number who answered the questionnaire</p><p>Demographics of participants.</p

Positive and negative predictive value of OSTAi index and NOF 2013.

<p>a, T-score ≤-2.5 at femoral neck, total hip, or lumbar spine;</p><p>b, Positive predictive value;</p><p>c, Sensitivity;</p><p>d, Negative predictive value;</p><p>e, Specificity</p><p>Positive and negative predictive value of OSTAi index and NOF 2013.</p

The ROC curves for comparison.

<p>(a)The ROC curves for final model variables. ROC curves of two-variable model (age and weight), three-variable model, four-variable model, and total seven variables in predicting osteoporosis (b)The ROC curves for the OSTAi index and NOF 2013 recommendations.</p

Regression coefficients for the univariate and multiple variable model.

<p>*SE: standard error</p><p>Regression coefficients for the univariate and multiple variable model.</p

Disposition of participants.

<p>Participants whose BMD was missing at any one site for any reason and participants with extreme values (deviating from the mean by more than three times the standard deviation) were excluded from data analysis.</p