17 research outputs found
Downregulation of Potential Tumor Suppressor miR-203a by Promoter Methylation Contributes to the Invasiveness of Gastric Cardia Adenocarcinoma
<p>Like many tumor suppressor genes, some miRNA genes harboring CpG islands undergo methylation-mediated silencing. In the study, we found significant downregulation and proximal promoter methylation of miR-203a and miR-203b in gastric cardia adenocarcinoma (GCA) tissues. The methylation status of miR-203a and miR-203b in tumor tissues was negatively correlated with their expression level. GCA patients in stage III and IV with reduced expression or hypermethylation of miR-203a demonstrated poor patient survival. In all, miR-203a and miR-203b may function as tumor suppressive miRNAs, and reactivation of miR-203a may have therapeutic potential and may be used as prognostic marker for GCA patients.</p
CsPb<sub>0.9</sub>Sn<sub>0.1</sub>IBr<sub>2</sub> Based All-Inorganic Perovskite Solar Cells with Exceptional Efficiency and Stability
The emergence of perovskite solar
cells (PSCs) has generated enormous
interest in the photovoltaic research community. Recently, cesium
metal halides (CsMX<sub>3</sub>, M = Pb or Sn; X = I, Br, Cl or mixed
halides) as a class of inorganic perovskites showed great promise
for PSCs and other optoelectronic devices. However, CsMX<sub>3</sub>-based PSCs usually exhibit lower power conversion efficiencies (PCEs)
than organicāinorganic hybrid PSCs, due to the unfavorable
band gaps. Herein, a novel mixed-Pb/Sn mixed-halide inorganic perovskite,
CsPb<sub>0.9</sub>Sn<sub>0.1</sub>IBr<sub>2</sub>, with a suitable
band gap of 1.79 eV and an appropriate level of valence band maximum,
was prepared in ambient atmosphere without a glovebox. After thoroughly
eliminating labile organic components and noble metals, the all-inorganic
PSCs based on CsPb<sub>0.9</sub>Sn<sub>0.1</sub>IBr<sub>2</sub> and
carbon counter electrodes exhibit a high open-circuit voltage of 1.26
V and a remarkable PCE up to 11.33%, which is record-breaking among
the existing CsMX<sub>3</sub>-based PSCs. Moreover, the all-inorganic
PSCs show good long-term stability and improved endurance against
heat and moisture. This study indicates a feasible way to design inorganic
halide perovskites through energy-band engineering for the construction
of high-performance all-inorganic PSCs
Data_Sheet_2_Analysis of shared ceRNA networks and related-hub genes in rats with primary and secondary photoreceptor degeneration.docx
IntroductionPhotoreceptor degenerative diseases are characterized by the progressive death of photoreceptor cells, resulting in irreversible visual impairment. However, the role of competing endogenous RNA (ceRNA) in photoreceptor degeneration is unclear. We aimed to explore the shared ceRNA regulation network and potential molecular mechanisms between primary and secondary photoreceptor degenerations.MethodsWe established animal models for both types of photoreceptor degenerations and conducted retina RNA sequencing to identify shared differentially expressed long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs). Using ceRNA regulatory principles, we constructed a shared ceRNA network and performed function enrichment and proteināprotein interaction (PPI) analyses to identify hub genes and key pathways. Immune cell infiltration and drugāgene interaction analyses were conducted, and hub gene expression was validated by quantitative real-time polymerase chain reaction (qRT-PCR).ResultsWe identified 37 shared differentially expressed lncRNAs, 34 miRNAs, and 247 mRNAs and constructed a ceRNA network consisting of 3 lncRNAs, 5 miRNAs, and 109 mRNAs. Furthermore, we examined 109 common differentially expressed genes (DEGs) through functional annotation, PPI analysis, and regulatory network analysis. We discovered that these diseases shared the complement and coagulation cascades pathway. Eight hub genes were identified and enriched in the immune system process. Immune infiltration analysis revealed increased T cells and decreased B cells in both photoreceptor degenerations. The expression of hub genes was closely associated with the quantities of immune cell types. Additionally, we identified 7 immune therapeutical drugs that target the hub genes.DiscussionOur findings provide new insights and directions for understanding the common mechanisms underlying the development of photoreceptor degeneration. The hub genes and related ceRNA networks we identified may offer new perspectives for elucidating the mechanisms and hold promise for the development of innovative treatment strategies.</p
Highly Efficient Retention of Polysulfides in āSea Urchinā-Like Carbon Nanotube/Nanopolyhedra Superstructures as Cathode Material for Ultralong-Life LithiumāSulfur Batteries
Despite
high theoretical energy density, the practical deployment of lithiumāsulfur
(LiāS) batteries is still not implemented because of the severe
capacity decay caused by polysulfide shuttling and the poor rate capability
induced by low electrical conductivity of sulfur. Herein, we report
a novel sulfur host material based on āsea urchinā-like
cobalt nanoparticle embedded and nitrogen-doped carbon nanotube/nanopolyhedra
(Co-NCNT/NP) superstructures for LiāS batteries. The hierarchical
micromesopores in Co-NCNT/NP can allow efficient impregnation of sulfur
and block diffusion of soluble polysulfides by physical confinement,
and the incorporation of embedded Co nanoparticles and nitrogen doping
(ā¼4.6 at. %) can synergistically improve the adsorption of
polysulfides, as evidenced by beaker cell tests. Moreover, the conductive
networks of Co-NCNT/NP interconnected by nitrogen-doped carbon nanotubes
(NCNTs) can facilitate electron transport and electrolyte infiltration.
Therefore, the specific capacity, rate capability, and cycle stability
of LiāS batteries are significantly enhanced. As a result,
the Co-NCNT/NP based cathode (loaded with 80 wt % sulfur) delivers
a high discharge capacity of 1240 mAh g<sup>ā1</sup> after
100 cycles at 0.1 C (based on the weight of sulfur), high rate capacity
(755 mAh g<sup>ā1</sup> at 2.0 C), and ultralong cycling life
(a very low capacity decay of 0.026% per cycle over 1500 cycles at
1.0 C). Remarkably, the composite cathode with high areal sulfur loading
of 3.2 mg cm<sup>ā2</sup> shows high rate capacities and stable
cycling performance over 200 cycles
High-Performance LiāSe Batteries Enabled by Selenium Storage in Bottom-Up Synthesized Nitrogen-Doped Carbon Scaffolds
Selenium
(Se) has great promise to serve as cathode material for rechargeable
batteries because of its good conductivity and high theoretical volumetric
energy density comparable to sulfur. Herein, we report the preparation
of mesoporous nitrogen-doped carbon scaffolds (NCSs) to restrain selenium
for advanced lithiumāselenium (LiāSe) batteries. The
NCSs synthesized by a bottom-up solution-phase method have graphene-like
laminar structure and well-distributed mesopores. The unique architecture
of NCSs can severe as conductive framework for encapsulating selenium
and polyselenides, and provide sufficient pathways to facilitate ion
transport. Furthermore, the laminar and porous NCSs can effectively
buffer the volume variation during charge/discharge processes. The
integrated composite of Se-NCSs has a high Se content and can ensure
the complete electrochemical reactions of Se and Li species. When
used for LiāSe batteries, the cathodes based on Se-NCSs exhibit
high capacity, remarkable cyclability, and excellent rate performance
Hierarchical Ternary Carbide Nanoparticle/Carbon Nanotube-Inserted NāDoped Carbon Concave-Polyhedrons for Efficient Lithium and Sodium Storage
Here,
we report a hierarchical Co<sub>3</sub>ZnC/carbon nanotube-inserted
nitrogen-doped carbon concave-polyhedrons synthesized by direct pyrolysis
of bimetallic zeolitic imidazolate framework precursors under a flow
of Ar/H<sub>2</sub> and subsequent calcination for both high-performance
rechargeable Li-ion and Na-ion batteries. In this structure, Co<sub>3</sub>ZnC nanoparticles were homogeneously distributed in in situ
growth carbon nanotube-inserted nitrogen-doped carbon concave-polyhedrons.
Such a hierarchical structure offers a synergistic effect to withstand
the volume variation and inhibit the aggregation of Co<sub>3</sub>ZnC nanoparticles during long-term cycles. Meanwhile, the nitrogen-doped
carbon and carbon nanotubes in the hierarchical Co<sub>3</sub>ZnC/carbon
composite offer fast electron transportation to achieve excellent
rate capability. As anode of Li-ion batteries, the electrode delivered
a high reversible capacity (ā¼800 mA h/g at 0.5 A/g), outstanding
high-rate capacity (408 mA h/g at 5.0 A/g), and long-term cycling
performance (585 mA h/g after 1500 cycles at 2.0 A/g). In Na-ion batteries,
the Co<sub>3</sub>ZnC/carbon composite maintains a stable capacity
of 386 mA h/g at 1.0 A/g without obvious decay over 750 cycles and
a superior rate capability (ā¼500, 448, and 415 mA h/g at 0.2,
0.5, and 1.0 A/g, respectively)
Correction to Highly Efficient Retention of Polysulfides in āSea-Urchinā-Like Carbon Nanotube/Nanopolyhedra Superstructures as Cathode Material for Ultralong-Life LithiumāSulfur Batteries
Correction to Highly Efficient Retention of Polysulfides
in āSea-Urchinā-Like Carbon Nanotube/Nanopolyhedra Superstructures
as Cathode Material for Ultralong-Life LithiumāSulfur Batterie
Data_Sheet_1_Analysis of shared ceRNA networks and related-hub genes in rats with primary and secondary photoreceptor degeneration.ZIP
IntroductionPhotoreceptor degenerative diseases are characterized by the progressive death of photoreceptor cells, resulting in irreversible visual impairment. However, the role of competing endogenous RNA (ceRNA) in photoreceptor degeneration is unclear. We aimed to explore the shared ceRNA regulation network and potential molecular mechanisms between primary and secondary photoreceptor degenerations.MethodsWe established animal models for both types of photoreceptor degenerations and conducted retina RNA sequencing to identify shared differentially expressed long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs). Using ceRNA regulatory principles, we constructed a shared ceRNA network and performed function enrichment and proteināprotein interaction (PPI) analyses to identify hub genes and key pathways. Immune cell infiltration and drugāgene interaction analyses were conducted, and hub gene expression was validated by quantitative real-time polymerase chain reaction (qRT-PCR).ResultsWe identified 37 shared differentially expressed lncRNAs, 34 miRNAs, and 247 mRNAs and constructed a ceRNA network consisting of 3 lncRNAs, 5 miRNAs, and 109 mRNAs. Furthermore, we examined 109 common differentially expressed genes (DEGs) through functional annotation, PPI analysis, and regulatory network analysis. We discovered that these diseases shared the complement and coagulation cascades pathway. Eight hub genes were identified and enriched in the immune system process. Immune infiltration analysis revealed increased T cells and decreased B cells in both photoreceptor degenerations. The expression of hub genes was closely associated with the quantities of immune cell types. Additionally, we identified 7 immune therapeutical drugs that target the hub genes.DiscussionOur findings provide new insights and directions for understanding the common mechanisms underlying the development of photoreceptor degeneration. The hub genes and related ceRNA networks we identified may offer new perspectives for elucidating the mechanisms and hold promise for the development of innovative treatment strategies.</p
DataSheet1_Gastrodin ameliorates the lipopolysaccharide-induced neuroinflammation in mice by downregulating miR-107-3p.xlsx
Background: Neuroinflammation plays a pivotal role in the pathogenesis of Central Nervous System (CNS) diseases. The phenolic glucoside gastrodin (GAS), has been known to treat CNS disorders by exerting anti-inflammatory activities. Our aim was to investigate the potential neuroprotective mechanisms of GAS on lipopolysaccharide (LPS)-induced mice.Methods: Male C57BL/6J mice were treated by LPS, before which GAS was adminisrated. The behavior tests such as forced swim test, tail suspension test, and elevated plus maze were performed to evaluate depressive-anxiety-like behaviors. A high-throughput sequencing (HTS) analysis was performed to screen out distinctive miRNAs which were validated using quantitative real-time PCR. Then, miRNA agomir or NC was injected stereotaxically into hippocampus of mice to explore the role of miRNA on GAS in response to LPS. Furthermore, Immunofluorescence and the hematoxylin and eosin (H&E) staining were employed to observe the cellular morphology. The protein levels of pro-inflammatory factors were evaluated by western blot. Finally, the target mRNA of miRNA was predicted using bioinformatics analysis. GO and KEGG enrichment analyses were conducted to clarify the potential function of target protein, which were visualized by bubble charts.Results: The behavioral data showed that mice in the LPS group had obvious depressive-anxiety-like behaviors, and 100Ā mg/kg GAS could improve these behavioral changes and alleviate the levels of pro-inflammatory cytokines in the hippocampus when mice were exposed to LPS for 6Ā h. Meanwhile, LPS-induced microglia and astrocyte activation in the CA1, CA2, CA3, and DG regions of the hippocampus were also reversed by GAS. Furthermore, miR-107-3p were screened out and verified for GAS in response to LPS. Importantly, miR-107-3p overexpression negatively abrogated the neuroprotective effects of GAS. Moreover, KPNA1 might be the target molecular of miR-107-3p. KPNA1 might regulate 12 neuroinflammation-related genes, which were mainly involved in cytokineāmediated signaling pathway.Conclusion: These results suggested that GAS might alleviate the LPS-induced neuroinflammation and depressive-anxiety-like behaviors in mice by downregulating miR-107-3p and upregulating the downstream target KPNA1. The indicates miR-107-3p may provide a new strategy for the treatment of CNS diseases.</p
In Situ Thermal Synthesis of Inlaid Ultrathin MoS<sub>2</sub>/Graphene Nanosheets as Electrocatalysts for the Hydrogen Evolution Reaction
Herein,
we report a unique thermal synthesis method to prepare a novel two-dimensional
(2D) hybrid nanostructure consisting of ultrathin and tiny-sized molybdenum
disulfide nanoplatelets homogeneously inlaid in graphene sheets (MoS<sub>2</sub>/G) with excellent electrocatalytic performance for HER. In
this process, molybdenum oleate served as the source of both molybdenum
and carbon, while crystalline sodium sulfate (Na<sub>2</sub>SO<sub>4</sub>) served as both reaction template and sulfur source. The
remarkable integration of MoS<sub>2</sub> and graphene in a well-assembled
2D hybrid architecture provided large electrochemically active surface
area and a huge number of active sites and also exhibited extraordinary
collective properties for electron transport and H<sup>+</sup> trapping.
The MoS<sub>2</sub>/G inlaid nanosheets deliver ultrahigh catalytic
activity toward HER among the existing electrocatalysts with similar
compositions, presenting a low onset overpotential approaching 30
mV, a current density of 10 mA/cm<sup>2</sup> at ā¼110 mV, and
a Tafel slope as small as 67.4 mV/dec. Moreover, the strong bonding
between MoS<sub>2</sub> nanoplatelets and graphene enabled outstanding
long-term electrochemical stability and structural integrity, exhibiting
almost 100% activity retention after 1000 cycles and ā¼97% after
100āÆ000 s of continuous testing (under static overpotential
of ā0.15 V). The synthetic strategy is simple, inexpensive,
and scalable for large-scale production and also can be extended to
diverse inlaid 2D nanoarchitectures with great potential for many
other applications