1 research outputs found
Identification of Antiangiogenic Potential and Cellular Mechanisms of Napyradiomycin A1 Isolated from the Marine-Derived <i>Streptomyces</i> sp. YP127
Angiogenesis is the process of new
blood vessel formation. Excessive
angiogenesis is a critical factor in the progression of cancer, macular
degeneration, and other chronic inflammatory diseases. When investigating
the effects of crude extracts of cultured marine microorganisms, an
extract of the cultured <i>Streptomyces</i> sp. YP127 strain
was found to inhibit human umbilical vein endothelial cell (HUVEC)
tube formation. Bioassay-guided fractionation and spectroscopic data
analyses led to the identification of napyradiomycin A1 (<b>1</b>) as an antiangiogenic component of the extract. Compound <b>1</b> inhibited HUVEC tube formation in a concentration-dependent manner.
It inhibited endothelial cell proliferation but did not affect human
dermal fibroblast proliferation. Compound <b>1</b> also suppressed
migration and invasion of vascular endothelial cells. In addition,
compound <b>1</b> suppressed vascular endothelial cadherin expression
and increased the permeability of the endothelial cell membrane. These
results suggested that compound <b>1</b> modulates cell permeability
and inhibits the angiogenesis of endothelial cells