Sediment rate (SR, cm yr^-1) and mass accumulation rate (MAR, g cm^-2 yr^-1) plotted against age determined from ²¹⁰Pb (calendar year AD) of two sediment cores (SAF-1 and BAF-1) in Yancheng coastal wetland, China.

<p>Sediment rate (SR, cm yr^-1) and mass accumulation rate (MAR, g cm^-2 yr^-1) plotted against age determined from ²¹⁰Pb (calendar year AD) of two sediment cores (SAF-1 and BAF-1) in Yancheng coastal wetland, China.</p

Historical trend of BC flux and its potential source.

<p><b>(A)</b> Variation of BC flux from 1860 to 2013 reconstructed from two sediment cores (SAF-1 and BAF-1) in Yancheng coastal wetland, China. An obvious peaking period of BC flux is marked by red bar (last 20 years). Error bars represent the standard deviation (SD) of the mean of three parallel samples. <b>(B)</b> Variations of social and economic development indicators in Jiangsu province including gross domestic product (GDP), gross industrial production, total energy consumption and total sown area [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0129680#pone.0129680.ref049" target="_blank">49</a>] and total carbon emission (1995–2010) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0129680#pone.0129680.ref050" target="_blank">50</a>].</p

Photos of core sampling.

<p><b>(A)</b> A photo showing the sampling site scene at Yancheng coastal wetland and core sampling in the field. <b>(B)</b> and <b>(C)</b> Photos showing the sediment cores of SAF-1 and BAF-1. PVC pipes were cut in half to show the lithologic character in the laboratory.</p

Radioisotope results for ²¹⁰Pb and ¹³⁷Cs.

<p><b>(A)</b>²¹⁰Pb activity (Bq kg^-1), <b>(B)</b>²¹⁰Pb-inferred chronologies (yr) and <b>(C)</b>¹³⁷Cs activity (Bq kg^-1) plotted against mass depth (g cm^-2) for SAF-1 core. <b>(D)</b>²¹⁰Pb activity, <b>(E)</b>²¹⁰Pb-inferred chronologies and <b>(F)</b>¹³⁷Cs activity plotted against mass depth for BAF-1 core. Error bars represent 1 standard deviation (SD) from counting uncertainty.</p

Depth variations of physicochemical parameters of two sediment cores (SAF-1 and BAF-1) in Yancheng coastal wetland, China.

<p><b>(A)</b> Water content (%). <b>(B)</b> Dry bulk density (g cm^-3). <b>(C)</b> Mass magnetic susceptibility (10⁻⁸ m³ kg^-1).</p

Location of sampling site.

<p><b>(A)</b> China map showing the location of Yancheng city in Jiangsu province. <b>(B)</b> Yancheng map showing the National Nature Reserve area and the sampling site.</p

Depth variations of black carbon (BC, mg g^-1) of two sediment cores (SAF-1 and BAF-1) in Yancheng coastal wetland, China.

<p>An obvious peaking section of BC content is marked by red bar (the surface 15 cm). Error bars represent the standard deviation (SD) of the mean of three parallel samples.</p

Interaction between PC2 and filamins by GST pull-down.

<p>(<b>A</b>) Total expression of purified GST- or His-tagged proteins. Left panel, representative data obtained with an anti-GST antibody showing purified GST fusion proteins and buffer from <i>E. coli</i>. Right panel, IB imaging obtained with an anti-His antibody showing purified His-tagged FLNAC, FLNBC and FLNCC from <i>E. coli</i>. (<b>B</b>) <i>E. coli</i> lysates from cells expressing GST-PC2N, GST-PC2C, GST alone, BSA or binding buffer alone, were incubated with purified His-tagged FLNAC, FLNBC or FLNCC. Glutathione-agarose beads were used to pull down GST epitope binding proteins. The resultant (Bound) protein samples were immunoblotted with an anti-His antibody (upper panels) or an anti-GST antibody (lower panels) to indicate the effective GST fusion proteins participated in interaction with His-tagged proteins. Data are representative of six experiments. (<b>C</b>) Data were obtained under similar conditions as in panel B, except that we utilized purified GST-PC2N, GST-PC2C, GST alone, or the binding buffer alone, incubated with purified His-tagged FLNAC, FLNBC or FLNCC. Data are representative of six experiments.</p

Cellular localization of PC2.

<p>M2 and A7 human melanoma cells over-expressing GFP-PC2 were grown on coverslips and incubated at 4°C overnight after fixation, with either anti-FLNA E-3 or anti-calnexin primary antibodies. The length of the white bar is 20 µm. (<b>A</b>) Subcellular co-localization of PC2 and FLNA. (<b>B</b>) Subcellular co-localization of PC2 and the ER marker calnexin (CNX).</p

Regulation of hST PC2 channel function by FLNA.

<p>Apical hST membranes, containing endogenous PC2 were reconstituted in a lipid bilayer electrophysiology system, as in Fig. 5. (<b>A</b>) Representative recordings of PC2 channel activity in the absence and presence of commercial FLNA (25 nM) added to the <i>cis</i> chamber. All-point histograms are shown on the right of each tracing, to indicate current amplitude. Data are representative of seven experiments. (<b>B</b>) Averaged open probability in the presence and absence of FLNA (N = 7). Bars were statistically different, with p<0.05. (<b>C</b>) Representative recording showing real-time inhibition of PC2 activity by addition of FLNA to the <i>cis</i> chamber.</p