Lodz City Tour: The Changing Nature of the Urban Restructuring of a Post-Socialist Industrial City

This paper describes the restructuring of Poland’s third-largest city, Lodz (Łódź). Once a thriving metropolitan hub of textile and clothing manufacturing, Lodz was confronted with the closure of its numerous factories in the early 1990s with Poland’s transition from a planned to a global market economy. The city suffered an abrupt and dramatic decline followed by a redefinition of its economic, spatial and social structures. The paper focuses on the major economic and spatial phenomena taking place in the city in the three decades after the economic collapse. Using an evolutionary approach, it identifies and discusses the gradual shift of Lodz’s restructuring. We claim that the restructuring initially involved mostly organic and spontaneous initiatives of dynamic local and non-local actors, with minor and circumstantial involvement from the local authorities. However, over time, the restructuring of Lodz has become influenced and driven by nationally and EU-set objectives and targeted urban policies, with urban and infrastructural projects as driving vehicles.Jakub Zasina’s portion of this work was supported by the National Science Centre in Poland (grant agreement no. 2016/23/N/HS4/03390)

Comparación de métodos de extracción de ADN de sangre para detectar ADN fúngico por PCR Comparison of different methods of DNA extraction from blood to detect fungal DNA by PCR

La infección fúngica invasora (IFI) está asociada a un alto índice de mortalidad, que alcanza el 50% debido a la frecuente falla en el tratamiento antifúngico. Existen dificultades para realizar un diagnóstico micológico rápido y certero dada la baja sensibilidad de los métodos convencionales, especialmente en pacientes neutropénicos y con SIDA. Numerosos métodos para diagnosticar infecciones micóticas basados en el estudio del ADN fúngico están actualmente en desarrollo. Nosotros evaluamos la utilidad de dos procedimientos de extracción y purificación del ADN fúngico presente en sangre para su posterior detección por PCR. Ambos métodos resultaron igualmente eficientes para obtener ADNs de óptima calidad y para realizar la técnica de PCR con los iniciadores universales para hongos ITS 1 e ITS 4.Invasive fungal infections (IFI) are associated with high mortality by reaching levels of 50%, and also with a significant failure in antifungical treatments. This fact mostly obeys to difficulties in obtaining a fast and accurate mycologic diagnosis due to the low sensitivity of conventional methods, mainly in neutropenic and AIDS patients. Various methods based on fungal DNA study are currently being used for the diagnosis of mycotic infections. We herein evaluated two procedures of extraction and purification of fungal DNA in blood for their use in PCR detection. Both of them showed equal efficiency in obtaining high performance DNA with universal primers ITS 1and ITS 4 as target