2 research outputs found
Site-Selective RNA Splicing Nanozyme: Dnazyme and RTCB Conjugates on a Gold Nanoparticle
Site-Selective RNA Splicing Nanozyme: DNAzyme and RtcB Conjugates on a Gold Nanoparticle
Modifying RNA through
either splicing or editing is a fundamental
biological process for creating protein diversity from the same genetic
code. Developing novel chemical biology tools for RNA editing has
potential to transiently edit genes and to provide a better understanding
of RNA biochemistry. Current techniques used to modify RNA include
the use of ribozymes, adenosine deaminase, and tRNA endonucleases.
Herein, we report a nanozyme that is capable of splicing virtually
any RNA stem–loop. This nanozyme is comprised of a gold nanoparticle
functionalized with three enzymes: two catalytic DNA strands with
ribonuclease function and an RNA ligase. The nanozyme cleaves and
then ligates RNA targets, performing a splicing reaction that is akin
to the function of the spliceosome. Our results show that the three-enzyme
reaction can remove a 19 nt segment from a 67 nt RNA loop with up
to 66% efficiency. The complete nanozyme can perform the same splice
reaction at 10% efficiency. These splicing nanozymes represent a new
promising approach for gene manipulation that has potential for applications
in living cells