Synthetic template scaffold build-up compared to native template selected by the algorithm.

<p>This is the comparison of the components of the synthetic template buildup for a chimeric FMDV, compared to the selected template compared to an individual scaffold template selection.</p

Consensus sequence buildup.

<p>Resolved sequence is selected by alignment of the candidate sequences for each of the thresholds. Sequence assembly is performed through selection of the individual resolved (non-ambiguous) nucleotides at the greatest MFI threshold.</p

Effect of variable Mean Fluorescent Intensity (MFI) on the identification of nucleotides in the consensus output.

<p>(A) △ 85% Power (Correct); ▲ 85% Power (Unidentified); ◇ 70% Power (Correct); ◆ 70% Power (Unidentified); □ 35% Power (Correct); ■ 35% Power (Unidentified); ○ 20% Power (Correct); ● 20% Power (Unidentified). Decreasing average MFI results in an overall reduction of correct nucleotides with an inverse increase in ambiguous nucleotides; seen as a shift in the 50% correct:unidentified ratio to a lower threshold. (B) ▲ 85% Power (Incorrect); ◆ 70% Power (Incorrect); ■ 35% Power (Incorrect); ● 20% Power (Incorrect) Effect of variable MFI on the percent of incorrectly identified nucleotides at different threshold cutoffs.</p

Comparison of specific misidentified nucleotides when comparing a chimeric virus to a “native template” (upper alignment) assembled sequence versus a “synthetic template” (lower alignment) assembled sequence to illustrate predicted indels and nucleotide error call improvement using the synthetic scaffold template.

<p>Comparison of specific misidentified nucleotides when comparing a chimeric virus to a “native template” (upper alignment) assembled sequence versus a “synthetic template” (lower alignment) assembled sequence to illustrate predicted indels and nucleotide error call improvement using the synthetic scaffold template.</p

Comparison of percent coverage and identity of microarray inferred interpretive sequences as compared to the predicted viral sequence record in Genbank.

<p>Sequence output from microarray was analyzed using the megaBLAST algorithm against Genbank. The identity of the highest scoring result was compared to the identity of the known viral FMD agents as confirmation of correct identification. Genbank accession numbers of the BLAST results (BLAST ID) are included for the expected nucleotide match, and the accession which identifies the sample sequence within Genbank (Sample ID) by the algorithm. All alignments were found to have significant E-scores of 0.0 by BLAST analysis.</p