2 research outputs found
Rapid Determination of Total Conjugated Linoleic Acid Content in Select Canadian Cheeses by <sup>1</sup>H NMR Spectroscopy
The
application of <sup>1</sup>H nuclear magnetic resonance (NMR)
spectroscopy to the measurement of conjugated linoleic acid (CLA)
content in the lipid fraction of dairy products is both a novel and
inviting alternative to traditional methods such as gas chromatography
(GC), which can require time-consuming sample derivatization. In this
work, a newly developed, rapid, and reliable lipid extraction protocol
was combined with simple, nondestructive <sup>1</sup>H NMR spectroscopic
analysis to measure the total CLA content in CLA standards and in
various Canadian cheeses from conventional, organic, and grass-fed
dairy sources. The total CLA concentrations (mg/g cheese) obtained
using these new extraction and analysis methods were consistent with
amounts found using the modified Folch extraction and GC analysis
(correlation coefficient of 0.948). Results showed that cheeses from
exclusively grass-fed dairy cows were significantly higher in total
CLA content than either conventional or organic cheese