EVI1 as a Prognostic and Predictive Biomarker of Clear Cell Renal Cell Carcinoma

The transcription factor EVI1 plays an oncogenic role in several types of neoplasms by promoting aggressive cancer features. EVI1 contributes to epigenetic regulation and transcriptional control, and its overexpression has been associated with enhanced PI3K-AKT-mTOR signaling in some settings. These observations raise the possibility that EVI1 influences the prognosis and everolimus-based therapy outcome of clear cell renal cell carcinoma (ccRCC). Here, gene expression and protein immunohistochemical studies of ccRCC show that EVI1 overexpression is associated with advanced disease features and with poorer outcome-particularly in the CC-e.3 subtype defined by The Cancer Genome Atlas. Overexpression of an oncogenic EVI1 isoform in RCC cell lines confers substantial resistance to everolimus. The EVI1 rs1344555 genetic variant is associated with poorer survival and greater progression of metastatic ccRCC patients treated with everolimus. This study leads us to propose that evaluation of EVI1 protein or gene expression, and of EVI1 genetic variants may help improve estimates of prognosis and the benefit of everolimus-based therapy in ccRCC

Thermal Analysis of Selected Polymer Materials for Precision Casting Models

This paper shows preliminary thermal analysis of 6 polymer materials which are commonly used or can be used as models for precision casting. Thermal analysis was carried out with use of derivatograph and allowed to determine two fundamental properties of examined materials - their phase transition during heating to the temperature of 800⁰C and mass of dry residue after burning which can be used to evaluate polymer residues remaining in the casting mould cavity. The subject of this paper is related to increasing participation of rapid prototyping methods in casting with use of printed models. Determination of changes in materials, in particular their linear and cubical expansion and analysis of dry residue after burning in casting mould cavity is extremely important for the construction of form crust and surface quality of casting. A problem is to design a polymer-based casting model which allows non-residue burning and simultaneously causes no cracking of ceramic form during heating

Analysis of the Manufacturing Process of Metal Layers in Ceramic Molds of the Burnt-out Models

This paper presents an innovative casting method that allows manufacturing the layers on the cast by applying the metallic coating inside the mold. In order to define the possibilities of formation of the metal layers on the castings attempts were made to modify the mold and the cast for models created of two selected plastic materials. The main issue was the choice of a suitable coating material and a casting alloy. The procedure was projected in a manner that would enable the model polymer coating to get into reaction with the cast liquid metal after the burn away. The adjustment of the casting cavity was obtained by the nickel coating. The pit was then filled with the aluminum alloy, when studying the aspects of diffusion and adhesion between the two metals (Ni and Al). On the assumption that nickel coating will generate a diffusion connection with the cast material, it is potential to create intermetallic phases of the system of Ni- Al that will modify the surface attributes of the molding surface. Such designed is to obtain, in a single operation, a ready casting part with a diffusion layer surface

The Analysis of Molding and Modification of the Ceramic Surfaces and Its Impact on the Roughness of Castings

The article presents an analysis of changes in the outer surface of the casting made in a modified ceramic form. The introduction of the modification in the inner surface of the form, as a metallic coating was intended to produce a high quality of the casting surface. To describe the results, the three parameters have been chosen to characterize the surface roughness, i.e. Ra, Rz and Rq. In implementing the popular method of casting was used, so – called the lost polymer models. The models made from different plastic materials are chemically and electrochemically coated with a layer of nickel. The next step was to remove the model from the form and pouring the liquid metal over it. The observation of the casting surface was performed by using the scanning electron microscope and a profilometer. As a result of these attempts a number of castings have been obtained with an outside layer generated of lower roughness than the casting made in the normal gypsum form. The proposed method can be called an innovative one because it reduces the technological cycle by one processing operation of investment casting. During the process of casting both the cast and the outer layer improving the properties of the manufactured product is being produced

Nickel layer deposition on investment casting polymer patterns

W artykule przedstawiono sposób modyfikacji powierzchni form ceramicznych na granicy rozdziału model wytapiany - ceramika poprzez zastosowanie warstwy niklowej na jednorazowym modelu wykonanym z określonego rodzaju polimeru, np. polimetakrylanu metylu (PMMA), a następnie nałożenie na tak przygotowany model warstw formy ceramicznej. Kolejne fazy zakładają usunięcie modelu ze skorupy ceramicznej, tak aby metaliczna warstwa pozostała wewnątrz formy. Do przygotowanej w ten sposób formy, po procesie wyżarzania, możliwe będzie wlanie ciekłego metalu o określonej temperaturze topnienia, np. stopu aluminium. W ten sposób można będzie uzyskać wysokiej jakości powierzchnię odlewu, o określonej chropowatości i braku wad powierzchniowych. Hipotezą badawczą postawioną w artykule jest takie zaprojektowanie metalicznej powłoki w formie ceramicznej, aby uzyskać jej lepszą zwilżalność ciekłym metalem niż przy zastosowaniu konwencjonalnych technik odlewania precyzyjnego.The article presents a method of modification of ceramic mould surface on the boundary between lost-wax model - ceramics by using a layer of nickel on a disposable model made of a specified type of polymer e.g. polymethyl methacrylate (PMMA) and then applying the layers of a ceramic mould to such a model. Successive phases consist in removing the model from the ceramic shell so that the metallic layer remains in the mould. After annealing it will be possible to pour liquid metal of a specific melting point e.g. aluminium alloy, to a mould prepared in this way. This way it is possible to obtain a high quality casting surface, of certain roughness and lack of surface defects. The research hypothesis stated in the article is to design a metallic coating in a ceramic form to get better wettability with molten metal than when using conventional techniques of precision casting

The Effect of Addition of Germanium to the Surface Phenomena in Silver Alloys

The article describes the impact of germanium on the course of surface phenomena in casting alloys of silver used in gold smithing. The aim of this works is to describe the assessment of resulting alloys, comparing the area of raw castings and the impact of the addition content of the alloy on the hardness of the samples. The evaluation also was subject to corrosion resistance of giving a comparison of their use in relations to traditional silver alloys

Determination of coordinates of control points in the Wieruszów District area

W pracy przedstawiono metodykę pomiaru i opracowania osnowy szczegółowej GPS III na obszarze powiatu wieruszowskiego. Pomiary 988 punktów wykonano metodą statyczną w ciągu dziesięciu dni z wykorzystaniem 12 odbiorników GPS Ashtech Z-XII, Z-Surveyor, Z-Xtreme. W pracy przedstawiono także analizy wyrównania i transformacji z układu ETRF-89 do państwowych układów współrzędnych: "1965" i "2000". Z uwagi na liczne zasłony drzew nad wyznaczanymi punktami, obserwacje GPS powtórzono na około trzydziestu punktach, aby uzyskać wymaganą dokładność i niezależną kontrolę pomiarów. Dodatkowo przy takiej liczbie odbiorników bardzo skuteczne okazały się analizy zamknięć przyrostów w trójkątach jak również warunki geometryczne nieoznaczoności pomiarów fazowych stosowane przy rozwiązaniach multistacyjnych.The paper presents the methodology of GPS measurements and data elaboration for control points in the Wieruszów District area. GPS measurements of 988 points were executed with the use of static method during ten days of measurements and twelve GPS receivers of Ashtech company i.e. Z-XII, Z-Surveyor, Z-Xtreme. The results and analyses of adjustment and transformation from ETRF-89 to the national coordinate systems: ,,1965" and ,,2000" were also presented. Due to bad observational conditions of GPS measurements there were about thirty points on which GPS measurements were repeated in order to achieve reliable and accurate results. Additionally, loop closures of baselines and geometric conditions of ambiguity network solutions were successfully useful before final adjustment