Paddles placement.

<p>Placement of paddles prior to IRE procedure.</p

Ablative characteristics.

<p>Ablative characteristics.</p

Density curve.

<p>Lower panel: A cross-section of the 2 circular paddles with a diameter of 25 mm and the plane in the middle between them (dashed line).Upper section: Current density curve in the middle plane between the 2 paddles for a tissue thickness of 7 millimeters and a nominal total ablation current of 1 Ampere.</p

Macroscopic changes.

<p>Macroscopic image of a cross-section of the ablated area after formalin fixation: the red dotted line indicates the border of the macroscopic damaged area in the central part of this section.</p

Microscopic image.

<p>Microscopic histology of porcine pancreatic tissue treated with a single application of 50 J; The location of both paddles is shown as two black bars with a length of 25 mm on both sides of the tissue. The parenchyma is marked as necrotic (red), transition area (orange); vital (green). Magnifications (H&E stain; scale bar 600 mircon.): vital pancreatic cells (left), vital cells in the upper right amid a completely necrotic area (middle) and a completely necrotic area characterized by nuclear dust.</p

Additional file 1: of Minimally invasive versus open distal pancreatectomy (LEOPARD): study protocol for a randomized controlled trial

SPIRIT 2013 checklist: recommended items to address in a clinical trial protocol and related documents. (DOC 112 kb