Schematic overview of fibrosis and adipose tissue in heart slices of control hearts.

<p>The results of the digital quantification in percentage of fibrosis or adipose tissue are shown using a color scale. The epicardial region has been excluded from the analysis. LV, left ventricle; RV, right ventricle; Ant., anterior; Post., posterior</p

Schematic heart slice overview of patients with a clinical phenotype of dilated cardiomyopathy.

<p>The results of the digital quantification in heart slices in percentage of fibrosis or adipose tissue are shown using a color scale. The epicardial region has been excluded from this overview. LV, left ventricle; RV, right ventricle; Ant., anterior; Post., posterior</p

Patient characteristics of the <i>PLN</i> p.Arg14del mutation carriers.

<p>SD, standard deviation; ICD, implantable cardioverter-defibrillator; CRT-D, cardiac resynchronization therapy defibrillator.</p

Overview of methodology.

<p>LV, left ventricle; RV, right ventricle; Ant., anterior; Post., posterior. <b>A,</b> gross showing a transverse heart slice of arrhythmogenic cardiomyopathy. <b>B,</b> transverse slice dissection scheme. <b>C</b>–<b>F,</b> examples of digital slide processing. Regions of interest are shown in orange lines (defining the epicardium, compact myocardium divided by an equidistant midline and trabeculated part). <b>C,</b> slide from the left ventricle posterior wall. <b>D,</b> slide C after digital processing. Red: cardiomyocytes. Blue: connective tissue. Pseudo green: adipose tissue. <b>E,</b> slide from the right ventricle lateral wall. <b>F,</b> slide E after digital processing. Red: cardiomyocytes. Blue: connective tissue. Pseudo green: adipose tissue.</p

Boxplots of mean fibrosis and mean adipose tissue in the different regions.

<p>Boxplots of mean percentage of fibrosis (A) and adipose tissue (B) per condition in 8 regions (C) corrected for surface area. Outliers are represented by small circles or stars. AC, arrhythmogenic cardiomyopathy; DCM, dilated cardiomyopathy; LV, left ventricle; RV, right ventricle; Ant., anterior; Post., posterior; 1 =  LV posterolateral wall; 2  =  LV posterior wall; 3  =  interventricular septum; 4  =  LV anterior wall; 5  =  LV anterolateral wall; 6  =  LV lateral wall; 7  =  RV posterior wall; 8  =  RV anterior wall.</p

Schematic heart slice overview of patients with a clinical phenotype of arrhythmogenic cardiomyopathy.

<p>The results of the digital quantification in heart slices in percentage of fibrosis or adipose tissue are shown using a color scale. The epicardial region has been excluded from this overview. LV, left ventricle; RV, right ventricle; Ant., anterior; Post., posterior</p