An Empirical study on the dynamic capabilities of public innovation cluster

Thesis(Master) -- KDI School: Master of Public Policy, 2021This study applies the dynamic capabilities concept introduced by Teece (2007) to examine the sustainable growth capability of the Daedeok Research Complex (DRC), a public innovation cluster in Korea. The thesis measures the three main components of dynamic capabilities - sensing, seizing, and transforming -as well as organizational cultural characteristics. This study conducted questionnaire surveys and semi-structured interviews with employees of public research institutions in DRC. The survey results were analyzed using a structural equation model (SEM), and the author conducted further interview about rejected hypothesis with three principal researchers and two directors who belong to the public IT institute. The findings indicated that the information searching and benchmarking capabilities of sensing had a significant effect on the internalization of knowledge of seizing, while networking relationships did not it. In turn, the organization’s internalization of knowledge had a significant effect on the resource adjustment or integration capability, but did not have a significant effect on the resource relocation or reconfiguration capability. In addition, organizational cultural characteristics had a positive mediating effect on the resource transformation capability. Through interviews with employees, the reason why networking relationship did not have significant effect on the organization’s internalization of knowledge was the culture of competition among members due to PBS. The reason why the internalization of knowledge did not have a significant effect on the resource relocation or reconfiguration was the complicated research project reporting structure and weak authority of the head of the institution. Overall, these findings suggest that the Korean government should focus on improving methods of evaluating public research and development (R&D) organizations, giving more authority to the heads of these institutions, and fostering an open organizational culture.1. Introduction 2. Literature Review 3. Research method and hypothesis 4. Data Analysis 5. ConclusionmasterpublishedJeongyun LE

Genetic and Molecular Characterization of a New EMS-Induced Mutant without the Third Glucose Moiety at the C-3 Sugar Chain of Saponin in Glycine max (L.) Merr.

Saponin, a secondary metabolite, is produced by various plant species, including soybean (Glycine max (L.) Merr.). Soybeans synthesize triterpenoid saponins, which are classified by their aglycone structure and sugar chain composition. Here, we characterized an ethyl methanesulfonate-induced mutant, PE1539, without saponin and with a glucose moiety at the third position of the C-3 sugar chain. The saponin phenotype of PE1539 is described by the accumulation of Ab-gamma g saponin and deficiency of Ab-alpha g saponin and DDMP-alpha g saponin, similar to a previously reported sg-3 mutant in soybean. Genetic analysis showed that the saponin phenotype of PE1539 is controlled by a recessive mutation. We mapped the gene responsible for the phenotype of PE1539 and the mapped region included Sg-3 (Glyma.10G104700). Further analysis of Sg-3 in PE1539 using DNA sequencing revealed a single-nucleotide substitution in the exon (G804A), resulting in a premature stop codon; thus, PE1539 produced a PSPG box-truncated protein. Saponin phenotype analysis of the F-2 population-from a cross between wild-type Uram and PE1539-showed that the phenotype of saponin was cosegregated with the genotype of Sg 3. Quantitative real-time PCR showed reduced expression of Sg-3 in PE1539 cells. Together, our data indicate that the saponin phenotype of PE1539 results from a mutation in Sg-3

The dispensability of VH-VL pairing and the indispensability of VL domain integrity in the IgG1 secretion process

Introduction: The CH1 domain of IgG antibodies controls assembly and secretion, mediated by the molecular chaperone BiP via the endoplasmic reticulum protein quality control (ERQC) mechanism. However, it is not clear whether the variable domains are necessary for this process.Methods: Here, we generated IgG1 antibodies in which the V domain (VH and/or VL) was either removed or replaced, and then assessed expression, assembly, and secretion in HEK293 cells.Results: All Ig variants formed a covalent linkage between the Cγ1 and Cκ, were successfully secreted in an assembled form. Replacement of the cognate Vκ with a non-secretory pseudo Vκ (ψVκ) hindered secretion of individual or assembled secretion of neither heavy chains (HCs) nor light chains (LCs). The ψLC (ψVκ-Cκ) exhibited a less folded structure compared to the wild type (wt) LC, as evidenced by enhanced stable binding to the molecular chaperone BiP and susceptibility to proteolytic degradation. Molecular dynamics simulation demonstrated dramatic alterations in overall structure of ψFab (Fd-ψLC) from wt Fab.Discussion: These findings suggest that V domains do not initiate HC:LC assembly and secretion; instead, the critical factor governing IgG assembly and secretion is the CH-CL pairing. Additionally, the structural integrity of the VL domain is crucial for IgG secretion. These data offer valuable insight into the design of bioactive molecules based on an IgG backbone

Bone Morphogenetic Protein Signaling: Implications in Urology

The bone morphogenetic proteins (BMPs), as members of the transforming growth factor-β (TGF-β) superfamily, not only control bone formation, but also regulate multiple key steps during embryonic development and differentiation. Furthermore, BMPs play critical roles in maintaining the homeostasis of the cardiovascular, pulmonary, reproductive, urogenital, and nervous systems in adult life. Like all members of the TGF-β superfamily, BMP signaling is mediated through a heteromeric complex of type I and type II transmembrane serine/threonine kinase receptors. The subsequent signal transduction cascade includes either the canonical Smad-dependent or non-canonical Smad-independent pathways. Reflecting the critical function of BMPs, BMP signaling is tightly regulated at multiple steps by various mechanisms including extracellular endogenous antagonists, neutralizing antibodies/extracellular soluble receptor domains, small molecule inhibitors, cytoplasmic inhibitory Smads, and transcriptional co-repressors. Recently, dorsomorphin, the first small molecule inhibitor of BMP signaling, was identified and suggested as a useful tool for dissecting the mechanisms of signaling pathways and for developing novel therapeutics for diverse human diseases that are related to the BMP signaling pathways. In this article, we discuss various mechanisms involved in regulating BMP signaling pathways and their implications for urology

Private Literacy Schools and Learning of Non-Enrollments in Colonial Korea : Based on the Oral History by Graduates of ‘Yangjeongwon’ and ‘Myoengwolsuk’-

The aim of this paper is to clarify the actual situation of learning of non-enrollments, and the features and circumstances of the educational activities of the Korean peoples, through the oral histories of private literacy school graduates in colonial Korea. Private literacy schools have been considered as a “ place of ethnic education ” of the Korean peoples mainly. In this paper, through be considered based on the position of the students of the literacy schools, to re-analyze the educational activities of the Korean peoples in the colonial period

Resting Metabolic Rate for Diagnosing Tae-Eum Sasang Type and Unraveling the Mechanism of Type-Specific Obesity

Increased resting metabolic rate (RMR), representing augmented energy expenditure, is a preferred physical characteristic; however, the Tae-Eum Sasang type, with a high incidence of obesity and metabolic diseases, has a higher RMR. This study scrutinized the physical characteristics of Sasang typology, a traditional Korean personalized medicine, to resolve this discrepancy, which can unravel the mechanism of Tae-Eum-type-specific obesity and improve the Tae-Eum Sasang-type diagnosis. A total of 395 healthy participants provided Sasang-type diagnosis using Sasang Constitutional Analysis Tool and physical features, including skeletal muscle mass, body fat mass, and RMR, along with those standardized using body weight. The Tae-Eum-type group showed significantly higher body weight, body mass index, body fat mass, and unstandardized RMR (kcal/day) than others, while their standardized measures of RMR per weigh (RMRw, kcal/day/kg) and percent skeletal muscle (PSM, %) were significantly lower. The logistic regression model revealed that the RMRw is pivotal for discriminating Tae-Eum type from others and explaining the developmental mechanism of Tae-Eum-type obesity. The aforementioned might provide a theoretical framework for Sasang-type diagnosis and Sasang-type-specific health promotion using bodily exercise and medical herbs

Study on the Extraction Method of Sub-Network for Optimal Operation of Connected and Automated Vehicle-Based Mobility Service and Its Implication

There have been enormous efforts to implement automated vehicle-based mobility (AVM) by considering smart infrastructure such as cooperative intelligent transportation system. However, there is lack of consideration on economical approach for an optimal deployment strategy of the AVM service and smart infrastructure. Furthermore, the influence of travel demand in service area has been ignored. We develop a new framework for maximizing the profit of connected and automated vehicle-based mobility (CAV-M) service using cost modeling and metaheuristic optimization algorithm. The proposed framework extracts an optimal sub-network, which is selected by a set of optimal links in the service area, and identifies an optimal construction strategy for the smart infrastructure depending on given operational design domain and travel demand. Based on service network analyses with varying demand patterns and volumes, we observe that the optimal sub-network varies with the combination of trip demand patterns and volumes. It is also found that the benefit of deploying the smart infrastructure is obtainable only when there are sufficient travel demands. Furthermore, the optimal sub-network is always superior to raw network in terms of economical profit, which suggests the proposed framework has great potential to prioritize road links in the target area for the CAV-M service

Breakage Characteristics of Heat-Treated Limestone Determined via Kinetic Modeling

In recent years, heat treatment has attracted attention as a means to improve the color sorting technology with the aim of improving the quality of low-grade limestone. The crucial stage in this technique is to evaluate the breakage characteristics of the sample materials after color sorting. In this study, the breakage characteristics of samples showing color differences after heat treatment and of the original raw material were investigated using a laboratory ball mill. The grinding was characterized using the population balance model. Specific rates of breakage and the primary breakage distribution were experimentally determined by first-order kinetic plots and the BII method. The breakage parameters were also back-calculated by employing a simplex method. Grinding of the three materials indicated first-order kinetics, and the experimental results were well described by the model with parameters obtained by back-calculating