Advanced Strategies for Net-Zero Energy Building: Focused on the Early Phase and Usage Phase of a Building’s Life Cycle

To cope with ‘Post-2020’, each country set its national greenhouse gas (GHG) emissions reduction target (e.g., South Korea: 37%) below its business-as-usual level by 2030. Toward this end, it is necessary to implement the net-zero energy building (nZEB) in the building sector, which accounts for more than 25% of the national GHG emissions and has a great potential to reduce GHG emissions. In this context, this study conducted a state-of-the-art review of nZEB implementation strategies in terms of passive strategies (i.e., passive sustainable design and energy-saving technique) and active strategies (i.e., renewable energy (RE) and back-up system for RE). Additionally, this study proposed the following advanced strategies for nZEB implementation according to a building’s life cycle: (i) integration and optimization of the passive and active strategies in the early phase of a building’s life cycle; (ii) real-time monitoring of the energy performance during the usage phase of a building’s life cycle. It is expected that this study can help researchers, practitioners, and policymakers understand the overall implementation strategies for realizing nZEB

A Prototype Design and Development of the Smart Photovoltaic System Blind Considering the Photovoltaic Panel, Tracking System, and Monitoring System

This study aims to design and develop the prototype models of the smart photovoltaic system blind (SPSB). To achieve this objective, the study defined the properties in three ways: (i) the photovoltaic (PV) panel; (ii) the tracking system; and (iii) the monitoring system. First, the amorphous silicon PV panel was determined as a PV panel, and the width and length of the PV panel were determined to be 50 mm and 250 mm, respectively. Second, the four tracker types (i.e., fixed type, vertical single-axis tracker, horizontal single-axis tracker, and azimuth-altitude dual-axis tracker) was applied, as well as the direct tracking method based on the amount of electricity generated as a tracking system. Third, the electricity generation and environmental conditions were chosen as factors to be monitored in order to evaluate and manage the technical performance of SPSB as a monitoring system. The prototype model of the SPSB is designed and developed for providing the electricity generated from its PV panel, as well as for reducing the indoor cooling demands through the blind’s function, itself (i.e., blocking out sunlight)

Antibacterial Activity of Chromomycins from a Marine-Derived Streptomyces microflavus

A marine-derived actinomycete (Streptomyces sp. MBTI36) exhibiting antibacterial activities was investigated in the present study. The strain was identified using genetic techniques. The 16S rDNA sequence of the isolate indicated that it was most closely related to Streptomyces microflavus. Furthermore, a new chromomycin A9 (1), along with chromomycin Ap (2), chromomycin A2 (3), and chromomycin A3 (4), were isolated from the ethyl acetate extract. Their structures were determined using extensive spectroscopic methods including 1D and 2D NMR, and HRMS, as well as comparisons with previously reported data. Compounds 1–4 showed potent antibacterial activities against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA). During a passage experiment, minimum inhibitory concentration (MIC) values for compounds 1–4 showed no more than a 4-fold increase from the starting MIC value, indicating that no resistance was detected over the 21 passages

Cytotoxic Scalarane Sesterterpenes from the Sponge Hyrtios erectus

Twelve new sesterterpenes along with eight known sesterterpenes were isolated from the marine sponge Hyrtios erectus collected off the coast of Chuuk Island, the Federated State of Micronesia. Based upon a combination of spectroscopic and computational analyses, these compounds were determined to be eight glycine-bearing scalaranes (1–8), a 3-keto scalarane (9), two oxidized-furan-bearing scalaranes (10 and 11), and a salmahyrtisane (12). Several of these compounds exhibited weak antiproliferation against diverse cancer cell lines as well as moderate anti-angiogenesis activities. The antiproliferative activity of new compound 4 was found to be associated with G0/G1 arrest in the cell cycle

PHF20 is crucial for epigenetic control of starvation-induced autophagy through enhancer activation

Autophagy is a catabolic pathway that maintains cellular homeostasis under various stress conditions, including conditions of nutrient deprivation. To elevate autophagic flux to a sufficient level under stress conditions, transcriptional activation of autophagy genes occurs to replenish autophagy components. Thus, the transcriptional and epigenetic control of the genes regulating autophagy is essential for cellular homeostasis. Here, we applied integrated transcriptomic and epigenomic profiling to reveal the roles of plant homeodomain finger protein 20 (PHF20), which is an epigenetic reader possessing methyl binding activity, in controlling the expression of autophagy genes. Phf20 deficiency led to impaired autophagic flux and autophagy gene expression under glucose starvation. Interestingly, the genome-wide characterization of chromatin states by Assay for Transposase-Accessible Chromatin (ATAC)-sequencing revealed that the PHF20-dependent chromatin remodelling occurs in enhancers that are co-occupied by dimethylated lysine 36 on histone H3 (H3K36me2). Importantly, the recognition of H3K36me2 by PHF20 was found to be highly correlated with increased levels of H3K4me1/2 at the enhancer regions. Collectively, these results indicate that PHF20 regulates autophagy genes through enhancer activation via H3K36me2 recognition as an epigenetic reader. Our findings emphasize the importance of nuclear events in the regulation of autophagy.N