Management Strategy for Open Innovation in Korean Biochip Industry

Due to the need to access external knowledge for new product development (NPD), open innovation has been widely used in the biochip industry. Since current resources owned by single firm do not have all the capabilities, NPD in biochip requires strong interdisciplinarity, wide diversity of technological knowledge, and integration capabilities. In the present study, management strategy for open innovation is investigated for NPD in the Korean biochip industry. Open innovation is classified in three steps: (1) switching phase about starting open innovation in the NPD initial stage; (2) implementation phase about open innovation management in the NPD middle stage; and (3) transition phase about change to close innovation in the NPD final stage. Three models for three phases are developed and then tested by carrying out surveys in the Korean biochip firms. In addition, the transition phase model is evaluated in the Korean bio-pharmaceutical firms. The switching phase model suggests that research capability and external trust are the main variables that affect switching cost, which relates to the perception of advantage of open innovation. The implementation phase model suggests that technological novelty affects degree of openness, which, in turn, relates to technological capability and firm performance. Furthermore, institutional-, environmental-, and firm characteristics affect the depth- and breadth of open innovation activity. The transition phase model suggests that knowledge connected with product innovation has an impact on the open-close transition tendency, which relates to perception of advantage of close innovation. Based on the results for three phases of open innovation, we propose the management strategy for open innovation during NPD. Therefore, based on the results of analyses of the proposed model, we can evaluate the factors that affect open innovation activity and develop an appropriate management strategy of open innovation for NPD of biochip

Assessment of Size-Dependent Antimicrobial and Cytotoxic Properties of Silver Nanoparticles

Nanoscale silver has been increasingly applied to commercial products for their antimicrobial function as antibiotics and disinfectants. In this work, the different sizes of silver nanoparticles (AgNPs) were studied not only in Methylobacterium spp. for their antimicrobial potential but also in human peripheral blood mononuclear cells (PBMCs) for their cytotoxicity in order to determine responses dependent on their particle size. Size controlled silver particles were prepared by chemical reduction of silver cations (Ag+) and then dispersed in water for their physicochemical characterization using transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potential measurements. To ascertain antimicrobial response, water-soluble silver nanoparticles were mixed into Methylobacterium spp. cultured for two days and the sample from the broth was spread on the agar plate for colony counting. 10 nm nanoparticles showed more antimicrobial activity than 100 nm particles at which concentrations were equivalently controlled. Increased cytotoxic effect of smaller silver nanoparticles was also observed in PBMCs cocultured with particles. Silver ions released from 10 nm particles might be correlated with upregulated antimicrobial and cytotoxic properties of AgNPs

Atomistic Engineering of Phonons in Functional Oxide Heterostructures

Engineering of phonons, that is, collective lattice vibrations in crystals, is essential for manipulating physical properties of materials such as thermal transport, electron-phonon interaction, confinement of lattice vibration, and optical polarization. Most approaches to phonon-engineering have been largely limited to the high-quality heterostructures of III–V compound semiconductors. Yet, artificial engineering of phonons in a variety of materials with functional properties, such as complex oxides, will yield unprecedented applications of coherent tunable phonons in future quantum acoustic devices. In this study, artificial engineering of phonons in the atomic-scale SrRuO3/SrTiO3 superlattices is demonstrated, wherein tunable phonon modes are observed via confocal Raman spectroscopy. In particular, the coherent superlattices led to the backfolding of acoustic phonon dispersion, resulting in zone-folded acoustic phonons in the THz frequency domain. The frequencies can be largely tuned from 1 to 2 THz via atomic-scale precision thickness control. In addition, a polar optical phonon originating from the local inversion symmetry breaking in the artificial oxide superlattices is observed, exhibiting emergent functionality. The approach of atomic-scale heterostructuring of complex oxides will vastly expand material systems for quantum acoustic devices, especially with the viability of functionality integration

SERS Application for Analysis of Live Single Cell

Monitoring changes of the protein contents and other macromolecules inside a living single cell during the key cellular processes such as cell differentiation, division, and apoptosis is a challenge for researchers. Raman spectroscopy is a powerful analytical technique for several biomedical applications that is rapid, reagent-free, and non-destructive while limited application with its weak signal. Surface-enhanced Raman scattering (SERS) technique is widely used to enhance the Raman signal (109-15 fold) by using surface Plasmon resonance of noble metal nanostructures (e.g. silver, gold, copper). SERS is a non-destructive spectroscopic method applied for biomedical samples. In this chapter, we will discuss the principles and fundamentals of SERS technique, theories and different strategies to obtain SERS signals such as immobilization of metal colloids on a substrate. Also, we show the SERS applications including the identification and discrimination of different types of cells (healthy and nonhealthy cells, e.g., cancer cells), and the interaction of cells with different drugs will also be discussed on monolayer bulk cells as well as on single-cell basis and for stem cell differentiation. In addition, we show the coupling of SERS with electrochemical techniques (EC-SERS) as spectroelectrochemical technique and its applications in biology, bioanalytical, and life science

PPM1A Controls Diabetic Gene Programming through Directly Dephosphorylating PPAR?? at Ser273

Peroxisome proliferator-activated receptor gamma (PPAR gamma) is a master regulator of adipose tissue biology. In obesity, phosphorylation of PPAR gamma at Ser273 (pSer273) by cyclin-dependent kinase 5 (CDK5)/extracellular signal-regulated kinase (ERK) orchestrates diabetic gene reprogramming via dysregulation of specific gene expression. Although many recent studies have focused on the development of non-classical agonist drugs that inhibit the phosphorylation of PPAR gamma at Ser273, the molecular mechanism of PPAR gamma dephosphorylation at Ser273 is not well characterized. Here, we report that protein phosphatase Mg2+/Mn2+-dependent 1A (PPM1A) is a novel PPAR gamma phosphatase that directly dephosphorylates Ser273 and restores diabetic gene expression which is dysregulated by pSer273. The expression of PPM1A significantly decreases in two models of insulin resistance: diet-induced obese (DIO) mice and db/db mice, in which it negatively correlates with pSer273. Transcriptomic analysis using microarray and genotype-tissue expression (GTEx) data in humans shows positive correlations between PPM1A and most of the genes that are dysregulated by pSer273. These findings suggest that PPM1A dephosphorylates PPAR gamma at Ser273 and represents a potential target for the treatment of obesity-linked metabolic disorders