Supernova Ejecta with Crystalline Silicate Dust in the Supernova Remnant MSH 15-52

IRAS 15099-5856 in the young supernova remnant (SNR) MSH 15-52 is the first and only SNR-associated object with crystalline silicate dust detected so far, although its nature and the origin of the crystalline silicate are still unclear. In this paper, we present high-resolution mid-infrared (MIR) imaging observations of the bright central compact source IRS1 of IRAS 15099-5856 to study the spatial distributions of gas and dust and the analysis of its Spitzer MIR spectrum to explore the origin of IRS1. The MIR images obtained with the T-ReCS attached on the Gemini South telescope show a complicated, inhomogeneous morphology of IRS1 with bright clumps and diffuse emission in [Ne II] 12.81 $\mu$m and Qa 18.30 $\mu$m, which confirms that IRS1 is an extended source externally heated by the nearby O star Muzzio 10, a candidate for the binary companion of the progenitor star. The Spitzer MIR spectrum reveals several ionic emission lines including a strong [Ne II] 12.81 $\mu$m line, but no hydrogen line is detected. We model the spectrum using the photoionization code CLOUDY with varying elemental composition. The elemental abundance of IRS1 derived from the model is close to that of SN ejecta with depleted hydrogen and enhanced metals, particularly neon, argon, and iron. Our results imply that IRS1 originates from the SN ejecta and suggest the possibility of the formation of crystalline silicate in newly-formed SN dust.Comment: 17 pages, 10 figures, submitted to Ap

Near-Infrared Spectroscopy of Infrared-Excess Stellar Objects in the Young Supernova Remnant G54.1+0.3

We present the results of broadband near-infrared spectroscopic observations of the recently discovered mysterious stellar objects in the young supernova remnant G54.1+0.3. These objects, which show significant mid-infrared-excess emission, are embedded in a diffuse loop structure of ~1' in radius. Their near-infrared spectra reveal characteristics of late O- or early B-type stars with numerous H and He I absorption lines, and we classify their spectral types to be between O9 and B2 based on an empirical relation derived here between the equivalent widths of the H lines and stellar photospheric temperatures. The spectral types, combined with the results of spectral energy distribution fits, constrain the distance to the objects to be 6.0 ± 0.4 kpc. The photometric spectral types of the objects are consistent with those from the spectroscopic analyses, and the extinction distributions indicate a local enhancement of matter in the western part of the loop. If these objects originate via triggered formation by the progenitor star of G54.1+0.3, then their formations likely began during the later evolutionary stages of the progenitor, although a rather earlier formation may still be possible. If the objects and the progenitor belong to the same cluster of stars, then our results constrain the progenitor mass of G54.1+0.3 to be between 18 and ~35 M_☉ and suggest that G54.1+0.3 was either a Type IIP supernova or, with a relatively lower possibility, Type Ib/c from a binary system

Sp1 up-regulates cAMP-response-element-binding protein expression during retinoic acid-induced mucous differentiation of normal human bronchial epithelial cells.

CREB [CRE (cAMP-response element)-binding protein] is an important transcription factor that is differentially regulated in cells of various types. We recently reported that RA (retinoic acid) rapidly activates CREB without using RARs (RA receptors) or RXRs (retinoid X receptors) in NHTBE cells (normal human tracheobronchial epithelial cells). However, little is known about the role of RA in the physiological regulation of CREB expression in the early mucous differentiation of NHTBE cells. In the present study, we report that RA up-regulates CREB gene expression and that, using 5\u27-serial deletion promoter analysis and mutagenesis analyses, two Sp1 (specificity protein 1)-binding sites located at nt -217 and -150, which flank the transcription initiation site, are essential for RA induction of CREB gene transcription. Furthermore, we found that CREs located at nt -119 and -98 contributed to basal promoter activity. Interestingly, RA also up-regulated Sp1 in a time- and dose-dependent manner. Knockdown of endogenous Sp1 using siRNA (small interfering RNA) decreased RA-induced CREB gene expression. However, the converse was not true: knockdown of CREB using CREB siRNA did not affect RA-induced Sp1 gene expression. We conclude that RA up-regulates CREB gene expression during the early stage of NHTBE cell differentiation and that RA-inducible Sp1 plays a major role in up-regulating human CREB gene expression. This result implies that co-operation of these two transcription factors plays a crucial role in mediating early events of normal mucous cell differentiation of bronchial epithelial cells

Microspinning: Local Surface Mixing via Rotation of Magnetic Microparticles for Efficient Small-Volume Bioassays

The need for high-throughput screening has led to the miniaturization of the reaction volume of the chamber in bioassays. As the reactor gets smaller, surface tension dominates the gravitational or inertial force, and mixing efficiency decreases in small-scale reactions. Because passive mixing by simple diffusion in tens of microliter-scale volumes takes a long time, active mixing is needed. Here, we report an efficient micromixing method using magnetically rotating microparticles with patterned magnetization induced by magnetic nanoparticle chains. Because the microparticles have magnetization patterning due to fabrication with magnetic nanoparticle chains, the microparticles can rotate along the external rotating magnetic field, causing micromixing. We validated the reaction efficiency by comparing this micromixing method with other mixing methods such as simple diffusion and the use of a rocking shaker at various working volumes. This method has the potential to be widely utilized in suspension assay technology as an efficient mixing strategy

Estimation of the Available Rooftop Area for Installing the Rooftop Solar Photovoltaic (PV) System by Analyzing the Building Shadow Using Hillshade Analysis

AbstractFor continuous promotion of the solar PV system in buildings, it is crucial to analyze the rooftop solar PV potential. However, the rooftop solar PV potential in urban areas highly varies depending on the available rooftop area due to the building shadow. In order to estimate the available rooftop area accurately by considering the building shadow, this study proposed an estimation method of the available rooftop area for installing the rooftop solar PV system by analyzing the building shadow using Hillshade Analysis. A case study of Gangnam district in Seoul, South Korea was shown by applying the proposed estimation method

Activation of spleen tyrosine kinase is required for TNF-α-induced endothelin-1 upregulation in human aortic endothelial cells

AbstractEndothelin-1 (ET-1) promotes atherosclerosis. We tested whether spleen tyrosine kinase (Syk) mediates tumor necrosis factor-α (TNF-α)-induced ET-1 upregulation in human aortic endothelial cells (HAECs) and sought to identify the signal pathways involved. TNF-α-induced reactive oxygen species (ROS) activated Syk and phosphatidylinositol 3-kinase (PI3K), which was required for the activation of AP-1 and subsequent ET-1 gene transcription. ROS mediated c-Jun NH2-terminal kinase (JNK) is also required for AP-1 activation, but Syk and PI3K regulated AP-1 activation independently of JNK. Through regulation of ET-1 production, Syk could be implicated in atherosclerosis