Robust Digital Predistortion in Saturation Region of Power Amplifiers

This paper proposes a digital predistortion (DPD) technique to improve linearization performance when the power amplifier (PA) is driven near the saturation region. The PA is a non-linear device in general, and the nonlinear distortion becomes severer as the output power increases. However, the PA’s power efficiency increases as the PA output power increases. The nonlinearity results in spectral regrowth, which leads to adjacent channel interference, and degrades the transmit signal quality. According to our simulation, the linearization performance of DPD is degraded abruptly when the PA operates in its saturation region. To relieve this problem, we propose an improved DPD technique. The proposed technique performs on/off control of the adaptive algorithm based on the magnitude of the transmitted signal. Specifically, the adaptation normally works for small and medium signals while it stops for large signals. Therefore, harmful coefficient updates by saturated signals can be avoided. A computer simulation shows that the proposed method can improve the linearization performance compared with the conventional DPD method in highly driven PAs

A Polynomial Digital Pre-Distortion Technique Based on Iterative Architecture

A digital predistortion (DPD) technique based on an iterative adaptation structure is proposed for linearizing power amplifiers (PAs). To obtain proper DPD parameters, a feedback path that converts the PA’s output to a baseband signal is required, and memory is also needed to store the baseband feedback signals. DPD parameters are usually found by an adaptive algorithm by using the transmitted signals and the corresponding feedback signals. However, for the adaptive algorithm to converge to a reliable solution, long feedback samples are required, which increases hardware complexity and cost. Considering that the convergence time of the adaptive algorithm highly depends on the initial condition, we propose a DPD technique that requires relatively shorter feedback samples. Specifically, the proposed DPD iteratively utilizes the short feedback samples in memory while keeping and using the DPD parameters found at the former iteration as the initial condition at the next iteration. Computer simulation shows that the proposed technique performs better than the conventional technique, as the former requires much shorter feedback memory than the latter

Cytokine responses of bovine macrophages to diverse clinical Mycobacterium avium subspecies paratuberculosis strains

BACKGROUND: Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of Johne's disease (JD) persistently infects and survives within the host macrophages. While it is established that substantial genotypic variation exists among MAP, evidence for the correlates that associate specific MAP genotypes with clinical or sub-clinical disease phenotypes is presently unknown. Thus we studied strain differences in intracellular MAP survival and host responses in a bovine monocyte derived macrophage (MDM) system. RESULTS: Intracellular survival studies showed that a bovine MAP isolate (B1018) and a human MAP isolate (Hu6) persisted in relatively higher numbers when compared with a sheep MAP isolate (S7565) at 24-hr, 48-hr and 96-hr post infection (PI). MDMs stimulated with B1018 up-regulated IL-10 at the transcript level and down-regulated TNFα at the protein and transcript levels compared with stimulations by the S7565 and Hu6. MDMs infected with Hu6 showed a down regulatory pattern of IL-10 and TNFα compared to stimulations by S7565. Cells stimulated with B1018 and Hu6 had low levels of matrix metalloprotease-3 (MMP3) and high levels of tissue inhibitor of metalloprotease-1 (TIMP1) at 96-hr PI relative to MDMs stimulated by S7565. CONCLUSION: Taken together, results suggest that the bovine (B1018) and the human (Hu6) MAP isolates lead to anti-inflammatory and anti-invasive pathways in the macrophage environment whereas the sheep (S7565) MAP isolate induces a pro-inflammatory pathway. Thus the infecting strain genotype may play a role in polarizing the host immune responses and dictate the clinicopathological outcomes in this economically important disease

Structure and evolution of online social relationships: heterogeneity in unrestricted discussions

With the advancement in the information age, people are using electronic media more frequently for communications, and social relationships are also increasingly resorting to online channels. While extensive studies on traditional social networks have been carried out, little has been done on online social networks. Here we analyze the structure and evolution of online social relationships by examining the temporal records of a bulletin board system (BBS) in a university. The BBS dataset comprises of 1908 boards, in which a total of 7446 students participate. An edge is assigned to each dialogue between two students, and it is defined as the appearance of the name of a student in the from- and to-field in each message. This yields a weighted network between the communicating students with an unambiguous group association of individuals. In contrast to a typical community network, where intracommunities (intercommunities) are strongly (weakly) tied, the BBS network contains hub members who participate in many boards simultaneously but are strongly tied, that is, they have a large degree and betweenness centrality and provide communication channels between communities. On the other hand, intracommunities are rather homogeneously and weakly connected. Such a structure, which has never been empirically characterized in the past, might provide a new perspective on the social opinion formation in this digital era

Improvements of motion vector in variational echo tracking technique by correction of initial guess

Póster presentado en: 3rd European Nowcasting Conference, celebrada en la sede central de AEMET en Madrid del 24 al 26 de abril de 2019

In vitro and in vivo protective efficacies of antibodies that neutralize the RNA N-glycosidase activity of Shiga toxin 2

<p>Abstract</p> <p>Backgound</p> <p>Shiga toxin 2 (Stx2), one of two Stx liberated by Stx-producing <it>Escherichia coli</it>, is composed of an A subunit monomer and a B subunit pentamer, and is directly linked with hemolytic uremic syndrome in children. The pentameric B subunit binds to its cell surface receptor Gb₃for toxin internalization, and the A subunit follows intracellular retrograde transport to the cytosol where its RNA <it>N</it>-glycosidase activity (RNA-NGA) shuts down the protein synthesis, and leads to cell death. The present study investigated the ability of 19 Stx2 A subunit-specific human monoclonal antibodies (HuMAbs) to neutralize the RNA-NGA, and the association this neutralizing activity with protection of HeLa cells and mice against Stx2-induced death.</p> <p>Results</p> <p>The HuMAbs that were stronger inhibitors of RNA-NGA were also better at neutralizing Stx2 mediated HeLa cell death, and those that were weaker inhibitors of RNA-NGA activity were also weaker in protecting HeLa cells. These results suggest that the ability of an A subunit-specific antibody to block the RNA-NGA of the toxin is directly related to its ability to neutralize Stx2-mediated HeLa cell death. However, with the exception of the best RNA-NGA blocking antibodies 5C12 and 2F10, the efficacies of antibody neutralization of RNA-NGA of Stx2 did not correlate with their <it>in vivo </it>protective efficacies. The HuMAb 6C3, which neutralized RNA N-glycosidase activity of Stx2 less effectively than the HuMAbs 6D8 and 6B7, protected 100% of the mice against Stx2 challenge at 50 μg/mouse dose. In contrast, the HuMAbs 6D8 and 6B7, which neutralized RNA N-glycosidase activity of Stx2 more effectively than 6C3, protected 20% and 0% mice at that dose, respectively.</p> <p>Conclusions</p> <p>The neutralization efficiency of the RNA-NGA of Stx2 by A subunit-specific antibodies correlate strongly with their abilities to protect HeLa cells against Stx2-mediated toxicity but only the strongest RNA-NGA-neutralizing antibodies correlate very well with both protecting HeLa cells and mice against Stx2 challenge.</p