Ethnic Minorities in Chinas Western Development Plan

Ethnic unrest within Chinas borders is commonly linked to problems of regional underdevelopment, yet ethnic minority issues have rarely been examined in the context of the PRCs regional economic development plans. What is the political logic of Chinas development in terms of minority policy? What resources are distributed, and where are they allocated? Here, I attempt to answer these questions in light of the Western Development Plan (WDP, xibudakaifa), Chinas largest regional development plan to date. I argue the WDP functions as a control mechanism of ethnic unrest by distributing resources selectively to key minority areas

Direct observation of DNA target searching and cleavage by CRISPR-Cas12a

Cas12a (also called Cpf1) is a representative type V-A CRISPR effector RNA-guided DNA endonuclease, which provides an alternative to type II CRISPR-Cas9 for genome editing. Previous studies have revealed that Cas12a has unique features distinct from Cas9, but the detailed mechanisms of target searching and DNA cleavage by Cas12a are still unclear. Here, we directly observe this entire process by using single-molecule fluorescence assays to study Cas12a from Acidaminococcus sp. (AsCas12a). We determine that AsCas12a ribonucleoproteins search for their on-target site by a one-dimensional diffusion along elongated DNA molecules and induce cleavage in the two DNA strands in a well-defined order, beginning with the non-target strand. Furthermore, the protospacer-adjacent motif (PAM) for AsCas12a makes only a limited contribution of DNA unwinding during R-loop formation and shows a negligible role in the process of DNA cleavage, in contrast to the Cas9 PAM.

Reconsidering repurposing: long-term metformin treatment impairs cognition in Alzheimer’s model mice

Metformin, a primary anti-diabetic medication, has been anticipated to provide benefits for Alzheimer’s disease (AD), also known as “type 3 diabetes”. Nevertheless, some studies have demonstrated that metformin may trigger AD pathology and even elevate AD risk in humans. Despite this, limited research has elucidated the behavioral outcomes of metformin treatment, which would hold significant translational value. Thus, we aimed to perform thorough behavioral research on the prolonged administration of metformin to mice: We administered metformin (300 mg/kg/day) to transgenic 3xTg-AD and non-transgenic (NT) C57BL/6 mice over 1 and 2 years, respectively, and evaluated their behaviors across multiple domains via touchscreen operant chambers, including motivation, attention, memory, visual discrimination, and cognitive flexibility. We found metformin enhanced attention, inhibitory control, and associative learning in younger NT mice (≤16 months). However, chronic treatment led to impairments in memory retention and discrimination learning at older age. Furthermore, metformin caused learning and memory impairment and increased levels of AMPKα1-subunit, β-amyloid oligomers, plaques, phosphorylated tau, and GSK3β expression in AD mice. No changes in potential confounding factors on cognition, including levels of motivation, locomotion, appetite, body weight, blood glucose, and serum vitamin B12, were observed in metformin-treated AD mice. We also identified an enhanced amyloidogenic pathway in db/db mice, as well as in Neuro2a-APP695 cells and a decrease in synaptic markers, such as PSD-95 and synaptophysin in primary neurons, upon metformin treatment. Our findings collectively suggest that the repurposing of metformin should be carefully reconsidered when this drug is used for individuals with AD

Risky neighbors and co-ethnic groups : the political logic of fiscal grants in China

How do China's ethnic politics and border concerns drive distributional outcomes? While many of China's 14 neighbors are widely considered unstable regimes, systematic studies of political risk diffusion have been surprisingly sparse. China's ethnic minorities, many of whom straddle borders with neighboring countries, have also been largely ignored in studies of fiscal distribution. I bridge this gap by focusing on China's transborder ethnic minorities (TEMs) - co-ethnic groups with external kin across the border. Using rare comprehensive center-to-local budget data from 1995 to 2003, I argue TEMs can easily create "porous borders" when neighbors experience political risk, spurring the Chinese leadership to give grants to prevent ethnic unrest. I also draw on 14 months of fieldwork to present in-depth case studies of the North Korea-China border are

Risky neighbors and co-ethnic groups : the political logic of fiscal grants in China

How do China's ethnic politics and border concerns drive distributional outcomes? While many of China's 14 neighbors are widely considered unstable regimes, systematic studies of political risk diffusion have been surprisingly sparse. China's ethnic minorities, many of whom straddle borders with neighboring countries, have also been largely ignored in studies of fiscal distribution. I bridge this gap by focusing on China's transborder ethnic minorities (TEMs) - co-ethnic groups with external kin across the border. Using rare comprehensive center-to-local budget data from 1995 to 2003, I argue TEMs can easily create "porous borders" when neighbors experience political risk, spurring the Chinese leadership to give grants to prevent ethnic unrest. I also draw on 14 months of fieldwork to present in-depth case studies of the North Korea-China border are

A Mask-Shaped Respiration Sensor Using Triboelectricity and a Machine Learning Approach toward Smart Sleep Monitoring Systems

Daily sleep monitoring is limited by the needs for specialized equipment and experts. This study combines a mask-shaped triboelectric nanogenerator (M-TENG) and machine learning for facile daily sleep monitoring without the specialized equipment or experts. The fabricated M-TENG demonstrates its excellent ability to detect respiration, even distinguishing oral and nasal breath. To increase the pressure sensitivity of the M-TENG, the reactive ion etching is conducted with different tilted angles. By investigating each surface morphology of the polytetrafluoroethylene films according to the reactive ion etching with different tilted angles, the tilted angle is optimized with the angle of 60° and the pressure sensitivity is increased by 5.8 times. The M-TENG can also detect changes in the angle of head and snoring. Various sleep stages can be classified by their distinctive electrical outputs, with the aid of a machine learning approach. As a result, a high averaged-classification accuracy of 87.17% is achieved for each sleep stage. Experimental results demonstrate that the proposed combination can be utilized to monitor the sleep stage in order to provide an aid for self-awareness of sleep disorders. Considering these results, the M-TENG and machine learning approach is expected to be utilized as a smart sleep monitoring system in near future

Establishment of a chemical tongue injury-recovery mouse model

Tongue epithelium is one of the most proliferative and regenerative epithelia in our body. However, tongue stem cell research is hampered partly by the lack of optimal animal models to study tongue injury, repair, and regeneration. Here, we establish a novel chemically induced tongue injury-recovery mouse model. Focal application of sodium hydroxide for a limited time led to the denudation of suprabasal layers, leaving the basal layer. Time course study revealed that tongue epithelial cells robustly proliferate over one week after the tongue injury. Importantly, we demonstrated that our novel mouse model could be employed in the lineage tracing of the tongue stem cells under the injury and repair process and further showed that tongue stem cells proliferate faster and generate larger clones in the injury condition than in the steady state condition. Our data indicate the development of a novel chemically induced tongue injury-recovery mouse model for tongue stem cell research, which will significantly facilitate the preclinical study for the pathogenesis and treatment of caustic ingestion. © 2022 Elsevier Inc.FALS

Medial and Lateral Canthal Reconstruction with an Orbicularis Oculi Myocutaneous Island Flap

Background The eyelid and canthal areas are common locations for cutaneous tumors. The medial canthus includes, among many other apparatuses, the canthal tendon and lacrimal canaliculi, and its characteristic thin and supple skin is hard to mimic and restore using tissue from other regions. Accordingly, reconstruction of the canthal area can prove challenging for surgeons. Although various methods, such as skin grafts and local flaps from adjacent regions, have been utilized for reconstructive purposes, they present known disadvantages. However, we were able to successfully reconstruct both lateral and medial canthal area defects by using orbicularis oculi myocutaneous island flaps. Methods Our study included seven patients who underwent medial or lateral canthal region reconstruction, using orbicularis oculi myocutaneous island flaps, between 2011 and 2014, following either cutaneous tumor excision or traumatic avulsion injury. Results Five patients had basal cell carcinoma, one had squamous cell carcinoma of the eyelid, and one had sustained a traumatic avulsion injury of the eyelid and canthal area. Entire flap loss was not observed in any patient, but one-a heavy smoker-showed partial flap loss, which healed with secondary intention and yielded acceptable results. Donor site morbidity was not observed, and all patients were satisfied with their surgical outcomes. Conclusions The canthal regions can be successfully reconstructed with orbicularis oculi myocutaneous island flaps. These flaps offer several key advantages, including similarity in texture, color, and thickness to the recipient site and a negligible incidence of donor site morbidity