AFM imaging of chromatin arrays under different salt concentrations.

<p>Data showing arrays in A: 0 mM NaCl, B: 30 mM NaCl, C: 75 mM NaCl, D: 250 mM NaCl. Each panel displays a single array together with a height profile of a cross-section as indicated in dark blue. Pictures were chosen to best represent the average compaction level of the corresponding sodium chloride concentration.</p

Salt-dependent chromatin array compaction.

<p>Shown are histograms (green) of chromatin array compaction, i.e. volume/area. The arrays were imaged by AFM and analysed using a graph cut algorithm to extract the volume and area occupied by each array. Plotted is the volume over area ratio. In black the Gaussian fits for each histogram are presented. A: 0 mM NaCl, B: 20 mM NaCl, C: 30 mM NaCl, D: 50 mM NaCl, E: 75 mM NaCl, F: 110 mM NaCl, G: 250 mM NaCl, H: 500 mM NaCl.</p

Summary of the analysed AFM data.

<p>Summary of the analysed AFM data.</p

Synchronous Emission from Nanometric Silver Particles through Plasmonic Coupling on Silver Nanowires

We investigated silver nanowires using correlative wide-field fluorescence and transmission electron microscopy. In the wide-field fluorescence images, synchronous emission from different distinct positions along the silver nanowires was observed. The sites of emission were separated spatially by up to several micrometers. Nanowires emitting in such cooperative manner were then also investigated with a combination of transmission electron microscopy based techniques, such as high-resolution, bright-field imaging, electron diffraction, high-angle annular dark-field imaging, and energy-dispersive X-ray spectroscopy. In particular, analyzing the chemical composition of the emissive areas using energy-dispersive X-ray spectroscopy led to the model that the active emissive centers are small silver clusters generated photochemically and that individual clusters are coupled <i>via</i> surface plasmons of the nanowire

Synchronous Emission from Nanometric Silver Particles through Plasmonic Coupling on Silver Nanowires

We investigated silver nanowires using correlative wide-field fluorescence and transmission electron microscopy. In the wide-field fluorescence images, synchronous emission from different distinct positions along the silver nanowires was observed. The sites of emission were separated spatially by up to several micrometers. Nanowires emitting in such cooperative manner were then also investigated with a combination of transmission electron microscopy based techniques, such as high-resolution, bright-field imaging, electron diffraction, high-angle annular dark-field imaging, and energy-dispersive X-ray spectroscopy. In particular, analyzing the chemical composition of the emissive areas using energy-dispersive X-ray spectroscopy led to the model that the active emissive centers are small silver clusters generated photochemically and that individual clusters are coupled <i>via</i> surface plasmons of the nanowire

Synchronous Emission from Nanometric Silver Particles through Plasmonic Coupling on Silver Nanowires

We investigated silver nanowires using correlative wide-field fluorescence and transmission electron microscopy. In the wide-field fluorescence images, synchronous emission from different distinct positions along the silver nanowires was observed. The sites of emission were separated spatially by up to several micrometers. Nanowires emitting in such cooperative manner were then also investigated with a combination of transmission electron microscopy based techniques, such as high-resolution, bright-field imaging, electron diffraction, high-angle annular dark-field imaging, and energy-dispersive X-ray spectroscopy. In particular, analyzing the chemical composition of the emissive areas using energy-dispersive X-ray spectroscopy led to the model that the active emissive centers are small silver clusters generated photochemically and that individual clusters are coupled <i>via</i> surface plasmons of the nanowire

Dorothea Lutherinna Helena Stränigstie to Carl Linnaeus

Dorothea Lutherinna Helena Stränigstie to Carl Linnaeu

Synchronous Emission from Nanometric Silver Particles through Plasmonic Coupling on Silver Nanowires

We investigated silver nanowires using correlative wide-field fluorescence and transmission electron microscopy. In the wide-field fluorescence images, synchronous emission from different distinct positions along the silver nanowires was observed. The sites of emission were separated spatially by up to several micrometers. Nanowires emitting in such cooperative manner were then also investigated with a combination of transmission electron microscopy based techniques, such as high-resolution, bright-field imaging, electron diffraction, high-angle annular dark-field imaging, and energy-dispersive X-ray spectroscopy. In particular, analyzing the chemical composition of the emissive areas using energy-dispersive X-ray spectroscopy led to the model that the active emissive centers are small silver clusters generated photochemically and that individual clusters are coupled <i>via</i> surface plasmons of the nanowire

Synchronous Emission from Nanometric Silver Particles through Plasmonic Coupling on Silver Nanowires

We investigated silver nanowires using correlative wide-field fluorescence and transmission electron microscopy. In the wide-field fluorescence images, synchronous emission from different distinct positions along the silver nanowires was observed. The sites of emission were separated spatially by up to several micrometers. Nanowires emitting in such cooperative manner were then also investigated with a combination of transmission electron microscopy based techniques, such as high-resolution, bright-field imaging, electron diffraction, high-angle annular dark-field imaging, and energy-dispersive X-ray spectroscopy. In particular, analyzing the chemical composition of the emissive areas using energy-dispersive X-ray spectroscopy led to the model that the active emissive centers are small silver clusters generated photochemically and that individual clusters are coupled <i>via</i> surface plasmons of the nanowire

Fluorescence Polarization Studies of Dye-Labeled, Stalled-Packaging Complexes

<p>Dye-labeled, stalled-packaging complexes were attached to the surface of a flow chamber and excited using the total internal reflection microscope. The excitation polarization was rotated between s- and p-polarization with a frequency of 0.7 Hz. The emitted fluorescence was separated into s- and p-polarization, respectively, and simultaneously detected (black and red). The dye bleached after 22 s. The integration time per data point was 75 ms. a.u., arbitrary units.</p