4 research outputs found
Blocking IL-10R signalling increases cellular influx into the lungs and delays recovery during RSV infection.
<p>BALB/c mice were infected with 10<sup>6</sup> PFU RSV i.n. (day 0). Where indicated, mice were injected with anti-IL-10R antibody on day -1 (i.p.), 3 (i.p. and i.n) and 6 (i.p.) post RSV infection. Control groups were injected with rat IgG. (A) Illness was monitored daily by changes in weight for 8 days after RSV infection; the percentage of original weight is shown. (B) Viral titer was measured in the lung on day 4 post infection by quantifying RSV L gene copies by qPCR. (C) Total numbers of cells in the lung and BAL were enumerated on day 4 and 8 from naïve or RSV infected mice. (D) Total numbers of neutrophils in the BAL were quantified using differential cell counting of H&E stained cytospins slides on day 4 and 8 post infection. (E) Total numbers of NK cells (CD3<sup>−</sup> NKp46<sup>+</sup>) and CD3-gated, CD4<sup>+</sup>Foxp3<sup>−</sup> and CD8<sup>+</sup> T cells in the lung were quantified using flow cytometry on day 4 and 8 post RSV infection. Error bars indicate the SEM. The data are representative of two independent experiments with n = 4–5 mice per group.</p
Lack of IL-10 increases cellular influx into the lungs and delays recovery during RSV infection.
<p>BALB/c mice and IL-10<sup>−/−</sup> mice were infected with 10<sup>6</sup> PFU RSV i.n. (day 0). (A) Illness was monitored daily by changes in weight for 14 days after RSV infection; percentage of original weight (day 0) is shown. (B) Copies of the RSV L gene were quantified in the lung on day 4 post infection using qPCR. (C) Total numbers of cells in the lung and BAL were enumerated on day 4 and 8 from naïve or RSV infected mice. (D) Total numbers of neutrophils in the BAL were quantified using differential cell counting of H&E stained cytospins slides on day 4 and 8 post infection. (E) Total numbers of NK cells (CD3<sup>−</sup> NKp46<sup>+</sup>) and CD3 gated CD4<sup>+</sup>Foxp3<sup>−</sup> and CD8<sup>+</sup> T cells in the lung were quantified using flow cytometry on day 4 and 8 post RSV infection. Error bars indicate the SEM. The data is representative of three independent experiments with n = 4–5 mice per group.</p
Increased frequency of IFN-γ-expressing T cells in IL-10<sup>−/−</sup> mice after RSV infection.
<p>BALB/c mice were infected with 10<sup>6</sup> PFU RSV i.n. (day 0). CD3<sup>+</sup> gated, CD4<sup>+</sup> and CD8<sup>+</sup> T cells from lungs on day 8 post RSV infection were analyzed by flow cytometry. Representative plots of intracellular IFN-γ expression after a 3 hr PMA/ionomycin restimulation are shown. In addition, quantifications of the frequencies of IFN-γ expression in CD4<sup>+</sup> T cells and CD8<sup>+</sup> T cells are shown. The data are representative of three independent experiments with n = 4–5 mice per group.</p
Lack of IL-10 increases chemokine and cytokine production in the airways during RSV infection.
<p>BAL samples from BALB/c and IL-10<sup>−/−</sup> mice were analyzed for indicated cytokines and chemokines on day 4 and 8 after RSV infection using Luminex. A group of naive BALB/c controls are included in the day 4 data. Error bars indicate the SEM. The data are pooled from two independent experiments with n = 4–5 mice per group.</p