MOESM1 of Occurrence of cardiorespiratory diseases and impact on lifespan in Swedish Irish Wolfhounds: a retrospective questionnaire-based study

Additional file 1: Questionnaire. A translated English version of the questionnaire distributed to owners of purebred Irish Wolfhounds born during 2006–2008

Manhattan plot of the genome-wide association study for dilated cardiomyopathy in Irish wolfhounds from Europe using a general linear model analysis.

<p>The genome-wide p-values (–log10 p-values) for the SNP effect are plotted against marker position on each chromosome. X-axis indicates marker number. Chromosomes are differentiated by colours. Colours are given below the plot. Red line indicates threshold value of probabilit<i>y</i> for significant association with DCM. Blue line indicates threshold value of probability for suggestive association with DCM.</p

Summary of results for the genome-wide association study using a mixed model analysis for dilated cardiomyopathy in European Irish wolfhounds.

<p>Identified loci on dog chromosome (CFA), position in base pair (bp), SNP identification and localization, gene name (Gene) for intragenic SNPs, major and minor allele, the minor allele frequency (MAF) in all dogs, affected and unaffected dogs (MAF all, aff, unaff) odds ratios (OR) with lower and upper 95% confidence limits (-L, -U), −log10 p-value of a general linear model (GLM) and mixed model analysis (MMA) is given.</p

Supplement Figure 1

The stability of cardiac troponin I (cTnI) concentration in serum samples from three cats with hypertrophic cardiomyopathy (HCM) after storage in the dark at 20°C</p

Supplement Table 2

Auscultation, basic echocardiographic and laboratory variables in 96 healthy cats and 39 cats with hypertrophic cardiomyopathy (HCM)</p

Supplement Table 1

Auscultation, basic echocardiographic and laboratory variables in 96 healthy Birman, Domestic Shorthair (DSH), and Norwegian Forest (NF) cats</p

Supplement 1 Stability study

The concentration of cTnI decreased in serum samples stored at 20°C. After three days, the mean decrease was 4% in comparison with the initial value. Mean decrease was 14% after 5 days and 20% after 7 days (Supplement Figure 1). The cTnI concentration changed from -1 to 5% after three freeze-thaw cycles.</p

Population based SNP analysis of the domestic cat.

<p>Number of monomorphic SNPs (left) and SNPs with a minor allele frequency 0< MAF <0.05 (right). Legend corresponds to the ten chromosomal regions.</p

Fraction of GWAS-informative SNP pairs at different distance classes in domestic cat breeds.

<p>Colored lines represent selected populations that depict upper, middle, and lower examples whereas the gray lines represent the remaining populations (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053537#pone.0053537.s012" target="_blank">Table S6</a>).</p

Extent of LD in domestic cats.

<p>a) Decay of <i>r²</i> value of autosomal chromosomes combined as a function of distance (Kb). Colored decay lines represent selected populations that depict upper, middle, and lower estimates of the LD measures. Gray decay lines represent the remaining populations. Filled circles indicate the point of 50% decay of <i>r²</i> maximum value. b) Distance in kilobases at which <i>r²</i> decays to 50% of the maximum value, which is the distance at <i>r²</i> ≈ 0.25. The distances correspond to the filled circles in (a).</p