Effect of PKA and PKC stimulation on Na,K-ATPase activity in membranes from rat and human skeletal muscle. a.

<p>Effect of the PKC activator PMA (phorbol 12-myristate 13-acetate) in membranes from rat and human skeletal muscle tested at 5 mM Na⁺, n = 6, SEM shown. <b>b.</b> Effect of the PKA activator cAMP (1 mM) in membranes from rat and human skeletal muscle tested at 5 mM Na⁺, n = 6 *: significantly different from control.</p

Comparison of the effects of purines on Na⁺ dependent Na,K-ATPase activity in membranes from rat and human skeletal muscle.

<p>The assay was performed at 0.5(control, dark column). <b>a.</b> Rat muscle. The bars represent the Na⁺ stimulated Na,K-ATPase activity (+SEM) at 40 mM Na⁺ (calculated from the difference between the activity at 0 and 40 mM Na⁺) after pre-incubation with purines (ADP, MeS-ADP, UTP, and UDP) in the presence of 0.5 mM ATP. Mean+SE shown. *indicates different from the activity with 0.5 mM ATP. #indicates different from UTP, n = 8. <b>b.</b> Human muscle. The effect of ADP, MeS-ATP, UTP, ATPγS, and UDP) in the presence of 0.5 mM ATP and 40 mM Na⁺ (control). In one series of experiments the P2Y antagonist suramin (100 µM) was added together with UTP, n = 6.</p

Fig 1 shows a schematic illustration of the positions of the primers and TaqMan probe sequences used for detection of the mRNA encoding the PGC-1α isoforms.

<p>FL primers and TaqMan probe detect the previously described PGC-1-α1/-a, PGC-1-α-b and PGC-1-α-c. The NT primers and Taqman probe detect the previously described NT-PGC-1-α-a, NT-PGC-1-α-b and PGC-1-α-c. The PGC-1α-A primers detect the previously described PGC-1-α1/-a and NT-PGC-1-α-a. The PGC-1α-B primers detect the previously described PGC-1-α-b, NT-PGC-1-α4/-b and PGC-1-α2. The PGC-1α-C primers detect the previously described PGC-1-α-c, NT-PGC-1-α-c and PGC-1-α3.</p

Primer and probe sequences used for real-time PCR.

<p>Primer and probe sequences used for real-time PCR.</p

The Effects of a Single Versus Three Consecutive Sessions of Football Training on Postprandial Lipemia: a Randomized, Controlled Trial in Healthy, Recreationally Active Males

Exercise frequency is important for maintaining health; however, its effects on postprandial responses remain largely unknown. Better understanding this during popular sports activities such as football may influence exercise habits. Therefore, the aim of the present study was to examine the effects of playing one single versus three consecutive days of 60-min small-sided football matches on postprandial lipemia. Fifteen males performed either one (1FOOT; n = 7) or three 60-min football (3FOOT; n = 8) sessions across an 8-day trial period. On day 1, a blood sample was collected at fasted (0 min) and 0.75, 2, 4, 6 h after a high-fat meal. Participants were then randomly allocated to the 1FOOT (day 7) or 3FOOT (days 5, 6, 7) condition. On day 8, they repeated the high-fat meal and blood sampling for 6 h following the meal. Postprandial total and incremental area under the curve (AUC, iAUC, respectively) were calculated. The postprandial triglyceride iAUC was 41% lower from pre- to post-measures for the 1FOOT (p Other Information Published in: Sports Medicine - Open License: https://creativecommons.org/licenses/by/4.0See article on publisher's website: http://dx.doi.org/10.1186/s40798-019-0212-1</p

Resting blood variables during 52 wks of soccer or resistance training in elderly men.

<p>Resting blood variables during 52 wks of soccer or resistance training in elderly men.</p

Anthropometric characteristics during 52 wks of soccer or resistance training in elderly men.

<p>Anthropometric characteristics during 52 wks of soccer or resistance training in elderly men.</p

List of anti-bodies.

<p>List of anti-bodies.</p

Representative immunofluorescent histochemical staining.

<p>Targets shown are; myofibre borders (top left/blue), capillaries (top right/light green) and myofibre type I/IIA (top right/dark green), myofibre type slow/type I (bottom left/red) and a merged over-lay af the above (bottom right). Scale bar = 100 μm.</p

Recruitment flow chart.

<p>Flow chart displaying the recruitment process of untrained healthy 65- to 75-year-old male participants. Number of participants after advertisement in local newspapers in the Copenhagen area of Denmark (n = 32), after medical examination (n = 28), randomization (n = 27), group allocation to soccer training (SG; n = 9), resistance training (RG; n = 9), or a control group (CG; n = 8).</p