10 research outputs found
Molecular environment and reactivity in gels and colloidal solutions under identical conditions
A PEG-Tyr block copolymer forms a kinetically stable colloidal solution in water at room temperature which undergoes an irreversible conversion to a gel phase upon heating. A micellar solution and a gel can therefore be studied under identical experimental conditions. This made it possible to compare physical properties and chemical reactivity of micelles and gels in identical chemical environments and under identical conditions. EPR spectra of the spin-labelled copolymer showed that tyrosine mobility in gels was slightly reduced compared to micelles. Chemical reactivity was studied using photochemical degradation of tyrosine and tyrosine dimerization, in the absence and in the presence of an Fe(iii) salt. The reactivity trends were explained by reduced tyrosine mobility in the gel environment. The largest reactivity difference in gels and micelles was observed for bimolecular dityrosine formation which was also attributed to the reduction in molecular mobility
Source-Sink Estimates of Genetic Introgression Show Influence of Hatchery Strays on Wild Chum Salmon Populations in Prince William Sound, Alaska
<div><p>The extent to which stray, hatchery-reared salmon affect wild populations is much debated. Although experiments show that artificial breeding and culture influence the genetics of hatchery salmon, little is known about the interaction between hatchery and wild salmon in a natural setting. Here, we estimated historical and contemporary genetic population structures of chum salmon (<i>Oncorhynchus keta</i>) in Prince William Sound (PWS), Alaska, with 135 single nucleotide polymorphism (SNP) markers. Historical population structure was inferred from the analysis of DNA from fish scales, which had been archived since the late 1960’s for several populations in PWS. Parallel analyses with microsatellites and a test based on Hardy-Weinberg proportions showed that about 50% of the fish-scale DNA was cross-contaminated with DNA from other fish. These samples were removed from the analysis. We used a novel application of the classical source-sink model to compare SNP allele frequencies in these archived fish-scales (1964–1982) with frequencies in contemporary samples (2008–2010) and found a temporal shift toward hatchery allele frequencies in some wild populations. Other populations showed markedly less introgression, despite moderate amounts of hatchery straying. The extent of introgression may reflect similarities in spawning time and life-history traits between hatchery and wild fish, or the degree that hybrids return to a natal spawning area. The source-sink model is a powerful means of detecting low levels of introgression over several generations.</p></div
Diagram of a model of genetic introgression based on the classic source-sink model of migration.
<p>Explanation of variables: <i>q<sub>l</sub></i> is the allele frequency at a locus in a source population and is assumed to be unchanging over <i>n</i> generations of introgression. <i>q<sub>n,i,l</sub></i> is the allele frequency at locus, <i>l,</i> in a wild sink population, <i>i</i> after <i>n</i> generations.</p
Competing models to detect DNA contamination among individuals (<i>k)</i> within a collection (<i>i</i>) across loci (<i>l</i>).
<p><sup></sup> Genotype index is a value assigned the apparent genotype observed during allele scoring. In uncontaminated and contaminated individuals, the probability of observing these apparent genotypes is estimated by Hardy-Weinberg expectations based on a single individual and on two individuals, respectively. See text for description.</p
Chum salmon production in Prince William Sound, Alaska hatcheries.
<p><sup>a</sup> Last used as brood stock in 1986.</p><p><sup>b</sup> Includes releases at Armin F. Koernig Hatchery and Port Chalmers remote release site.</p
Neighbor-joining tree of <i>F</i><sub>ST</sub> between chum salmon samples from Prince William Sound, Alaska.
<p>Numbers in the tree represent bootstrap support for a node.</p
STRUCTURE analysis of genotypes at 135 nuclear SNPs in chum salmon from Prince William Sound, Alaska.
<p>Individual assignments for contemporary (C) and historical (H) collections with <i>K</i> = 4.</p
Plots of versus for 135 SNP loci in chum salmon in Prince William Sound, Alaska.
<p>Dashed line represents observed curve and solid line represents expected curve without introgression. (a) Siwash Creek, (b) Wells River (c) Beartrap Creek, (d) Constantine Creek.</p
Estimates of genetic diversity and divergence (<i>F</i><sub>ST</sub>) between historical (H) and contemporary (C) samples of chum salmon from Prince William Sound, Alaska.
<p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081916#pone-0081916-t002" target="_blank">Table 2</a> for sample abbreviation. <i>F</i><sub>ST</sub> (below diagonal), expected heterozygosity <i>H</i><sub>e</sub> (diagonal in <b>bold</b>), and Probability of Fisher’s exact test over loci for selected comparisons (above diagonal) between historical (H) and contemporary (C) collections.<sup></sup> See </p><p><i>P</i><0.001.<sup></sup></p
Locations of chum salmon hatcheries and release sites and sampled natural-spawning sites in Prince William Sound, Alaska.
<p>Locations of chum salmon hatcheries and release sites and sampled natural-spawning sites in Prince William Sound, Alaska.</p