ADCC activity in BMS and plasma.

<p>Percent ADCC activity in BMS(A) and plasma (B). BMS was tested at a 1∶100 dilution and plasma at a 1∶1000 dilution. The subject ID for the corresponding ADCC measure is shown below each bar. The results are from duplicate testing and are an average of at least two independent experiments each done using effector cells from a single donor. nd indicates not done.</p

ADCC activity in relation to HIV-1 Env specific IgG titers in BMS.

<p>The Y-axis shows % ADCC activity in BMS (1∶100) and the X-axis shows the log₁₀ BM HIV-1 env specific IgG titers. Filled and open symbols represent transmitting and non-transmitting women, respectively. The trend line ‘Linear (ALL)’ is the regression line including both transmitting and non-transmitting women.</p

Neutralization potency of purified IgG and IgA from four mothers against heterologous virus.

<p>The graphs show percent neutralization versus BMS purified IgG (A) or IgA (B) dilution. The corresponding symbol for the data from each of the four mothers is shown in the upper right corner. Neutralization by IgG and IgA is represented by filled and open symbols, respectively. Results using pseudovirus generated with Q461.d1 env (HIV-1 in black lines) are shown in the left graph and with SIVMneCl8 (SIV in grey lines) are shown in the right graph. The 50% neutralization level is shown with a dotted line. The results are from duplicate testing and are representative of at least two independent experiments. The average IC50s for the two experiments for all 19 women is reported in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002739#ppat.1002739.s002" target="_blank">Table S1</a>.</p

Neutralization potency of plasma and BMS from four mothers against heterologous virus.

<p>The graphs show percent neutralization versus plasma (A) or BMS (B) dilution. Results using pseudovirus generated with heterologous Q461.d1 env (HIV-1 in black lines) are shown in the left graph and with SIVMneCl8 (SIV in grey lines) are shown in the right graph. The corresponding symbol for the data from each of the four mothers is shown in the upper right corner. The 50% neutralization level is shown with a dotted line. The results are from triplicate testing and are representative of at least two independent experiments. The average IC50s for the two experiments for all 19 women is reported in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002739#ppat-1002739-t001" target="_blank">Table 1</a>.</p

Neutralization potency of IgG and IgA from two mothers against autologous virus.

<p>Representative graphs showing percent neutralization versus BMS purified IgG or IgA dilution. (a) Neutralization by IgG and IgA fractions from subject MM471 and (b) neutralization by IgG and IgA fractions from subject MA411. IgG (filled square) and IgA (open square) responses against pseudovirus generated with autologous HIV-1 env are shown in black lines and against SIVMneCl8 (SIV) are shown in grey lines. The 50% neutralization level is shown with a dotted line. The results are from duplicate testing and are representative of at least two independent experiments.</p

The characteristics of transmitting and non-transmitting women in the study and the neutralization IC50s of their plasma and BMS.

a<p>Plasma neutralization assays were performed at a starting dilution of 1∶100; an IC50 of 50 was assigned in cases where 50% neutralization was not achieved.</p>b<p>BMS neutralization assays were performed at a starting dilution of 1∶20; an IC50 of 10 was assigned in cases where 50% neutralization was not achieved.</p>c<p>Viral Load.</p>d<p>Indicates week since delivery when infant was first HIV-1 DNA positive.</p>e<p>Indicates time-point after delivery at which BM sample was obtained.</p>f<p>Q461.d1.</p>g<p>Not done.</p>h<p>Not applicable.</p>i<p>Below detection.</p