Signal transduction pathway genes are required for female development.

<p>PP – <u>P</u>reviously<u> p</u>ublished data demonstrated that the gene was needed for female development.</p><p>NA – a deletion strain is <u>n</u>ot <u>a</u>vailable in the single gene deletion library.</p><p>NA(het) – the deletion strain in the single gene deletion library is a <u>het</u>erokaryon and a homokaryon isolate was <u>n</u>ot <u>a</u>vailable during the screening experiments.</p><p>RIP – a RIP experiment was used to verify that the gene is required for female development.</p><p>An * by the NCU number indicates that the gene is needed for CAT (conidia anastomosis tube) formation (a cell fusion phenotype) and is likely to be a component of either the MAK-1 or MAK-2 signal pathway.</p><p>A notation of “This report” in the reference information column indicates that the gene was either newly identified or verified by co-segregation and complementation analysis as being needed for <i>N. crassa</i> development by our experiments.</p><p>Signal transduction pathway genes are required for female development.</p

<i>Neurospora crassa</i> Female Development Requires the PACC and Other Signal Transduction Pathways, Transcription Factors, Chromatin Remodeling, Cell-To-Cell Fusion, and Autophagy

<div><p>Using a screening protocol we have identified 68 genes that are required for female development in the filamentous fungus <i>Neurospora crassa</i>. We find that we can divide these genes into five general groups: 1) Genes encoding components of the PACC signal transduction pathway, 2) Other signal transduction pathway genes, including genes from the three <i>N. crassa</i> MAP kinase pathways, 3) Transcriptional factor genes, 4) Autophagy genes, and 5) Other miscellaneous genes. Complementation and RIP studies verified that these genes are needed for the formation of the female mating structure, the protoperithecium, and for the maturation of a fertilized protoperithecium into a perithecium. Perithecia grafting experiments demonstrate that the autophagy genes and the cell-to-cell fusion genes (the MAK-1 and MAK-2 pathway genes) are needed for the mobilization and movement of nutrients from an established vegetative hyphal network into the developing protoperithecium. Deletion mutants for the PACC pathway genes <i>palA</i>, <i>palB</i>, <i>palC</i>, <i>palF</i>, <i>palH</i>, and <i>pacC</i> were found to be defective in two aspects of female development. First, they were unable to initiate female development on synthetic crossing medium. However, they could form protoperithecia when grown on cellophane, on corn meal agar, or in response to the presence of nearby perithecia. Second, fertilized perithecia from PACC pathway mutants were unable to produce asci and complete female development. Protein localization experiments with a GFP-tagged PALA construct showed that PALA was localized in a peripheral punctate pattern, consistent with a signaling center associated with the ESCRT complex. The <i>N. crassa</i> PACC signal transduction pathway appears to be similar to the PacC/Rim101 pathway previously characterized in <i>Aspergillus nidulans</i> and <i>Saccharomyces cerevisiae</i>. In <i>N. crassa</i> the pathway plays a key role in regulating female development.</p></div

Miscellaneous genes needed for female development.

<p>PP – <u>P</u>reviously<u> p</u>ublished data demonstrated that the gene was needed for female development.</p><p>NA – a deletion strain is <u>n</u>ot <u>a</u>vailable in the single gene deletion library.</p><p>NA(het) – the deletion strain in the single gene deletion library is a <u>het</u>erokaryon and a homokaryon isolate was <u>n</u>ot <u>a</u>vailable during the screening experiments.</p><p>A notation of “This report” in the reference information column indicates that the gene was identified as being needed for <i>N. crassa</i> development by our experiments.</p><p>Miscellaneous genes needed for female development.</p

PALA is localized to the small intracellular vesicles.

<p>GFP-tagged PALA was expressed under the regulation of the <i>ccg-1</i> promoter in a Δ<i>palA</i> isolate. The GFP fluorescent image of a germling is shown in the panel on the left (A). The DIC image of the same germling is shown in the right panel (B). The bar in the DIC image is 10 µm in length.</p

Genes from the PacC pathway are required for female development.

<p>A notation of “This report” in the reference information column indicates that the gene was identified as being needed for <i>N. crassa</i> development by our experiments.</p><p>Genes from the PacC pathway are required for female development.</p

Constitutively active PACC activates female development.

<p>Cells were inoculated onto agar slants and allowed to grow for 10 days at room temperature. The left panel (A) shows a Δ<i>palC</i> isolate on Vogel’s sucrose medium. The middle panel (B) shows a Δ<i>palC</i> isolate that has been transformed with the constitutively activated PACC construct growing on synthetic crossing medium. The right panel (C) shows a Δ<i>palC</i> isolate that has been transformed with the constitutively activated PACC construct growing on Vogel’s sucrose medium, a medium that represses female development. Note that the constitutively activated PACC caused protoperithecia production in the absence of PALC on both media. The arrows in the middle and right panels point to protoperithecia.</p

Autophagy genes required for female development.

<p>RIP – a RIP experiment was used to verify that the gene is required for female development.</p><p>A notation of “This report” in the reference information column indicates that the gene was identified as being needed for <i>N. crassa</i> development by our experiments.</p><p>Autophagy genes required for female development.</p

Perithecia grafting experiments.

<p>Small pieces of cellophane containing “graft” fertilized wild type protoperithecia were placed on mutant “host” vegetative hyphal networks grown on synthetic crossing medium. The “host” shown are: 1) Δ<i>fmf-1</i> (left panel/A) which shows a host supporting the development of the graft perithecia. 2) Δ<i>ada-1</i> (middle panel/B) which shows a host not supporting the development of the graft perithecia. 3) Δ<i>palA</i> (right panel/C) which shows the graft inducing protoperithecia in the Δ<i>palA</i> host. Arrows point to examples of perithecia on the cellophane (left panel/A) and protoperithecia induced in the host vegetative hyphal network (right panel/C).</p

Schematic representation of the <i>N. crassa</i> PACC pathway.

<p>The PACC signal transduction pathway elements found in <i>N. crassa</i>, and the model for how the pathway might function are depicted. The PALH and PALF proteins are thought to be found at the plasma membrane. PALH is a seven transmembrane receptor which is sensitive to environmental cues. PALF is an arrestin type protein that associates with PALH. PALF is phosphorylated and ubiquitinated in response to the environmental cues. These events lead to the endocytosis of the PALH/PALF complex. Following endocytosis, the PALH is directed into an ESCRT compartment, where it enters into a signaling complex containing PALA, PALB, PALC, and PACC. Within the signaling complex, PALB functions as a protease which cleaves PACC. This cleavage event removes a C-terminal inhibitory domain from the PACC transcription factor, and the processed PACC is released from the signaling complex. The activated PACC then enters the nucleus and directs transcriptional activity leading to the formation of the protoperithecium.</p

Transcription factors needed for female development.

<p>PP – <u>P</u>reviously<u> p</u>ublished data demonstrated that the gene was needed for female development.</p><p>NA – a deletion strain is <u>n</u>ot <u>a</u>vailable in the single gene deletion library.</p><p>NA (het) – the deletion strain in the single gene deletion library is a <u>het</u>erokaryon and a homokaryon isolate was <u>n</u>ot <u>a</u>vailable during the screening experiments.</p><p>Transcription factors needed for female development.</p