3D imaging of cells in scaffolds: direct labelling for micro CT.

The development of in-vitro techniques to characterise the behaviour of cells in biomedical scaffolds is a rapidly developing field. However, until now it has not been possible to visualise, directly in 3D, the extent of cell migration using a desktop X-ray microCT. This paper describes a new technique based on cell labelling with a radio opacifier (barium sulphate), which permits cell tracking without the need for destructive sample preparation. The ability to track cells is highlighted via a comparison of cell migration through demonstrator lyophilised collagen scaffolds with contrasting pore size and interconnectivity. The results demonstrate the ease with which the technique can be used to characterise the effects of scaffold architecture on cell infiltration

GAKTpore: Stereological Characterisation Methods for Porous Metal Foams in Biomedical Applications- Data

In this work, a rapid, efficient, and accurate methodology for 2D bulk porous structure analysis and pore morphology classification is proposed. The algorithm, “GAKTpore”, creates a morphology map allowing visualisation of spatial feature variation, which enables optimisation of pore sizes/shapes/ranges/dispersion within any porous structure. The micrographs used in this study with their data outputted from the GAKTpore algorithm and below. Micrographs are stored as .tiff files and data is outputted and saved as a .CSV file