Study participant treatments.

<p>¹Frequency (percentage of study participants for the category)</p><p>Study participant treatments.</p

Perceived Stress Levels Reported by Women Treated for Breast Cancer.

<p>¹The DASS21 descriptors/PSS-10 correlate values are based on Andreou, et al., 2011.</p><p>²N = Number of women providing values for this PSS-10 score category. PSS-10 values were not available for one women at visit 2 (case 2076) and one woman at visit 4 (case 2005).</p><p>Perceived Stress Levels Reported by Women Treated for Breast Cancer.</p

Summary of previous findings related to cancer/cancer treatment in which MN frequencies were evaluated in peripheral blood.

<p>Summary of previous findings related to cancer/cancer treatment in which MN frequencies were evaluated in peripheral blood.</p

Changes in MN/cytome abnormality frequencies associated with radiotherapy.

<p>At the baseline and 4 weeks (mid-chemo) time points, no significant differences in the chromosomal instability frequencies were observed between the women who did (white circles) or did not (black circles) receive radiation therapy. However, a statistically significantly increase in MN/cytome abnormality frequencies was observed at six months (compared to baseline) for the group of women receiving radiotherapy versus the group not receiving radiation (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133380#pone.0133380.t006" target="_blank">Table 6</a>). Also, for the subset of women who received radiotherapy, this increase in chromosomal instability values persisted at the 1 year time point (p<0.0001).</p

Changes in MN/cytome abnormality frequencies over the 1 year follow-up period based on the women’s chemotherapy regimen.

<p>When compared to baseline values, the frequencies of acquired chromosomal instability values were significantly higher for at least 1-year following the initiation of treatment (visit 4) for the women who received the TAC (<i>p</i><0.0001) or TC (<i>p</i> = 0.014) regimens, but not the TCH treatment (<i>p</i> = 0.0884). TAC = black circles; TC = white circles; TCH = black triangles.</p

Study participants’ tumor characteristics.

<p>¹Frequency (percentage of study participants for the category)</p><p>Study participants’ tumor characteristics.</p

Increased MN/cytome abnormality frequencies were significantly associated with higher levels of perceived stress.

<p>The results of the linear mixed effects model are illustrated in this figure, which shows the increasing trend line of the MN/cytome frequency with increasing PSS (p = 0.0129). The PSS categories proposed by Andreou, et al., 2011 are shown on the X axis (a subset of PSS range); the MN/cytome frequencies (predicted values from linear mixed effects model) are shown on the Y axis.</p

Examples of giemsa stained binucleates with MN.

<p>Mitotic cells were blocked at cytokinesis (but did complete karyokinesis) to result in binucleates. In the figure to the left (A) a single micronucleus is present. In the right figure (B) 2 micronuclei have been excluded from the parental cell.</p

Mixed effects linear model fitting assessment of predictive associations of variables with peripheral blood micronuclei/cytome abnormality frequencies¹^,².

<p>¹The base model, which was determined by the study design, was: MN frequency = Visit + Chemotherapy (3 types) + Radiation therapy + Visit by Chemotherapy* + Visit by Radiation therapy* with the study subject being a random effect</p><p>²Variables having predictive <i>p</i> values greater than 0.25 in the initial stepwise model included: Age (p = 0.9599); BMI (<i>p</i> = 0.2719); Income (<i>p</i> = 0.3728); Menopausal status (<i>p</i> = 0.4964); Alcohol consumption (<i>p</i> = 0.0816); Tumor grade (<i>p</i> = 0.7563); Tumor stage (<i>p</i> = 0.2981); Luminal A (<i>p</i> = 0.9807); HER2 positive (<i>p</i> = 0.6360); and Surgery (<i>p</i> = 0.3092)</p><p>*The visit by chemo and visit by radiation interaction variables allowed these values to differ across time points.</p><p>Mixed effects linear model fitting assessment of predictive associations of variables with peripheral blood micronuclei/cytome abnormality frequencies<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133380#t006fn001" target="_blank">¹</a>^,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133380#t006fn002" target="_blank">²</a>.</p

MN frequencies for discordant MZ twin pairs and age-matched controls.

<p>The mean level is indicated by a horizontal bar for controls (mean [SD] 14.2 [9.4]), CSA nonexposed (14.9 [5.6]) and CSA exposed (22.0 [11.3]) twins. CSA exposure status was significant in this combined sample (<i>P</i><0.001), while there was no indication of an additional effect attributable to the familial environment (<i>P = </i>0.406) based on results from generalized mixed-effect models.</p