CONCENTRATION OF GLUCOSE IN SERUM OF SIMMENTAL COWS FROM DIFFERENT GEOGRAPHIC AREAS

The aim of this paper is to examine the concentration of glucose in serum of Simmental cows from different geographic areas (lowland and mountain). The total number of tested animals was 42 healthy cows, by 21 at each farm, aged 3-5 years. The studies were conducted in two periods: autumn-winter (November-December), and repeated in spring (April-May) on the same cows. The concentration of glucose in the blood (serum) of tested animals does not exceed the physiological value

INSEMINATION INDEX AND FERTILIZATION SUCCESS IN HOLSTEIN`S CATTLE RACE AFTER RETENTION OF THE PLACENTA

Reproductive capabilities are significant economic characteristics and they are given special attention and strives to maintain at a satisfactory level or even improve by different zootechnical measures. Reproduction in Holstein cows is an introduction to production and are indirectly linked, and better reproductive characteristics in cows also give positive economic effects. Significant reproductive characteristics are the index of insemination and the success of insemination after calving. The research work was based on proving the effect of residual placenta and its retention on the mentioned reproductive properties. The first group of cows X1 consisted of cows with normal calves and numbered 34 cows, and were characterized by on time plunged placenta, with an average index of insemination of 1.2. The insemination index in the group of cows X2 containing 20 cows with residual placenta, and where reteniting of placenta was up to 30 hours after calving, was 2.1. The largest oscillation index had a group of cows X3 that counted 14 heads, where the placenta retention was made after 30 hours from the calving moment with a 4.86 index. Detailed analysis of the insemination index showed the data for the success rate of insemination, for the group X1 from the first attempt was 79.41% and from the second was 20.59%. Group X2 has an insemination performance for the first attempt of 45.0%, the second attempt was 30.0%, the third 10.0% and the fourth was 15.0%. Cows that have placental retention after 30 hours of calving, X3, the success rate of insemination is very low, and for the first attempt it was 14.3%, the second and the third to 7.15% while for the fourth and subsequent attempts was 50.0%. The best reproductive indicators had a group of cows with normal calves and calves removed on time

BASIC PHENOTYPIC MEASURES OF SERBIAN HOUND AND SERBIAN TRICOLOUR HOUND POPULATION IN BOSNIA AND HERZEGOVINA

Along with indigenous hound breed Barak, a large number of hounds used in hunting in Bosnia and Herzegovina belongs to breeds Serbian Hound, Serbian Tricolour Hound and Posavatz Hound. So far, the population of these breeds in Bosnia and Herzegovina has not been the subject of research. The aim of this paper is to describe basic phenotypic parameters of Serbian Hound and Serbian Tricolour Hound from Bosnia and Herzegovina, to determine whether the obtained values meets the values defined by standards for these breeds, and is there any significant difference between the populations of dogs in Serbia and Bosnia and Herzegovina. The study was conducted on 38 dogs, including 20 Serbian Hounds (11 males and 9 females), and 18 Serbian Tricolour Hounds (10 males and 8 females). The following parameters were measured on each dog: the height at withers, the body length the length of head, the length of scull, the length of muzzle, the girth of chest. The measurements were performed using Litin rod, moving scale with nonius and the tape. Based on the results obtained from this research, it can be concluded that the Serbian Hound does not have large deviations from the values defined by standard, nor the differences between the populations of dogs in home country and Bosnia and Herzegovina. In the Serbian Hound, a significant statistical difference is observed between the genders in following parameters: the height at withers, the length of head and the length of scull. In the Serbian Tricolour Hound, the males observed to be higher than height interval from standard for this breed, while the females remained in the values defined by standard. A very significant statistical differences between the genders were determined in all measured parameters for Serbian Tricolour Hound

Uticaj đubriva na morfološke karakteristike i prinos odabranog hibrida kukuruza

Basic significance of corn comes from plant characterstics, diversitiy of use and volume of production. Even though it is high on volume of production top list in the world, this production has low yield and insufficient profitability in B&H. The main task of annual research is to establish impact of fertilization on yield of selected hybrid corn from FAO 400 group. Research has established impact of various fertilization alternatives on chemical structure of kernel and yield of selected hybrid corn NS 444 with these fertilization variants: control, NPK (mineral fertilizer) ang organic fertilizer. When talking about quality parameters, for every way of fertilization content of raw proteins, fats, cellulose and ashes is defined. Acquired results were statistically processed by t-test analysis which showed that there are statistically significant differencies on 0,05 % level (p<0.005) between all average researched parameters.Osnovni značaj kukuruza proizilazi iz osobine biljke, raznovrsnosti upotrebe i obima proizvodnje. Iako po obimu proizvodnje u svijetu zauzima visoko mjesto, na području BiH ovu proizvodnju karakteriše izuzetno nizak prinos i nedovoljna profitabilnost. Glavni zadatak eksperimentalnog istraživanja jeste utvrditi uticaj đubriva na morfološke i hemijske osobine, kao i prinos odabranog hibrida kukuruza iz FAO skupine 400. U istraživanju je utvrđen uticaj različitih varijanti đubriva na morfološke osobine, hemijski sastav zrna i prinos odabranog hibrida kukuruza NS 444 sa slijedećim varijantama đubrenja: kontrola, NPK (mineralno đubrivo) i stajnjak (organsko đubrivo). Kada su u pitanju kvalitativni pokazatelji, za svaki način đubrenja, utvđen je sadržaj sirovih proteina, masti, celuloze i pepela. Dobijeni rezultati statistički su obrađeni analizom t-testa i utvrđeno je da između svih prosječnih istraživanih parametara postoje statistički značajne razlike na 0,05 % nivou (p<0,005)

Koncentracija Ca, P I Mg u serumu kod krava simentalske rase sa različitih geografskih područja

The aim of this paper is to examine the concentration Ca, P and Mg in serum of Simmental cows from different geographic areas (lowland and mountain). Total number of animals tested was 42 healthy cows, by 21 at each farm, aged 3-5 years. The researches were carried out in two periods: autumn-winter (November-December 2008), and repeated in spring (April-May 2009) on the same cows. The concentration of Ca, P and Mg in the blood (serum) from both locations was significantly higher in spring than in winter, but do not exceed the physiological value.Cilj ovog rada je da se ispita koncetracija Ca, P i Mg u serumu kod krava simentalske rase sa različitih geografskih područja (ravničarsko i planinsko). Ukupna broj ispitivanih životinja je 42 zdrave krave, po 21 na svakoj farmi, starosti 3-5 godina. Istraživanja su provedena u dva perioda: jesen-zima (novembar-decembar 2008), te ponovljena na istim kravam u proljeće (april-maj 2009). Koncentracija Ca, P i Mg u krvi (serumu) sa oba lokaliteta je značajno viša u proljeće nego u zimu, ali ne prelazi fiziološke vrijednosti

Evaluation of In Vitro Cytotoxic Potential of Avarol towards Human Cancer Cell Lines and In Vivo Antitumor Activity in Solid Tumor Models

The goal of this study was to determine the activity in vitro and in vivo of avarol, a sesquiterpene hydroquinone originating from the Dysidea avara sponge from the south Adriatic Sea, against different cancer cell lines and two types of mouse carcinoma. To investigate the in vitro cytotoxicity, a human cervix adenocarcinoma cell line (HeLa), human colon adenocarcinoma (LS174), human non-small-cell lung carcinoma (A549), and a normal human fetal lung fibroblast cell line (MRC-5) were used. The in vivo antitumor activity was investigated against two transplantable mouse tumors, the Ehrlich carcinoma (EC) and cervical cancer (CC-5). The effect of avarol on cancer cell survival, which was determined by the microculture tetrazolium test, confirmed a significant in vitro potency of avarol against the investigated cell lines, without selectivity towards MRC-5. The highest cytotoxicity was exhibited against HeLa cancer cells (10.22 &plusmn; 0.28 &mu;g/mL). Moreover, potent antitumor activity against two tumor models was determined, as the intraperitoneal administration of avarol at a dose of 50 mg/kg resulted in a significant inhibition of tumor growth in mice. After three administrations of avarol, a 29% inhibition of the EC growth was achieved, while in the case of CC-5, a 36% inhibition of the tumor growth was achieved after the second administration of avarol. Therefore, the results indicate that this marine sesquiterpenoid hydroquinone could be a promising bioactive compound in the development of new anticancer medicine

Evaluation of In Vitro Cytotoxic Potential of Avarol towards Human Cancer Cell Lines and In Vivo Antitumor Activity in Solid Tumor Models

The goal of this study was to determine the activity in vitro and in vivo of avarol, a sesquiterpene hydroquinone originating from the Dysidea avara sponge from the south Adriatic Sea, against different cancer cell lines and two types of mouse carcinoma. To investigate the in vitro cytotoxicity, a human cervix adenocarcinoma cell line (HeLa), human colon adenocarcinoma (LS174), human non-small-cell lung carcinoma (A549), and a normal human fetal lung fibroblast cell line (MRC-5) were used. The in vivo antitumor activity was investigated against two transplantable mouse tumors, the Ehrlich carcinoma (EC) and cervical cancer (CC-5). The effect of avarol on cancer cell survival, which was determined by the microculture tetrazolium test, confirmed a significant in vitro potency of avarol against the investigated cell lines, without selectivity towards MRC-5. The highest cytotoxicity was exhibited against HeLa cancer cells (10.22 ± 0.28 μg/mL). Moreover, potent antitumor activity against two tumor models was determined, as the intraperitoneal administration of avarol at a dose of 50 mg/kg resulted in a significant inhibition of tumor growth in mice. After three administrations of avarol, a 29% inhibition of the EC growth was achieved, while in the case of CC-5, a 36% inhibition of the tumor growth was achieved after the second administration of avarol. Therefore, the results indicate that this marine sesquiterpenoid hydroquinone could be a promising bioactive compound in the development of new anticancer medicine